جستجو و شناسایی هلیکوباکتر پلوروم در کبد و محتویات سکوم تعدادی از طیورگوشتی ارجاعی به درمانگاه‌های شهر تبریز، با استفاده از روش‌های کشت و PCR
محورهای موضوعی :
آسیب شناسی درمانگاهی دامپزشکی
سید محمد علوی شوشتری
1
,
افشین جوادی
2
,
غلامرضا زرینی
3
,
حمید میرزایی
4
,
شهرام حنیفیان
5
1 - دانشجوی دکترای تخصصی، گروه بهداشت مواد غذایی، دانشکده دامپزشکی، علوم پزشکی تبریز، دانشگاه آزاد اسلامی، تبریز، ایران.
2 - استاد گروه بهداشت مواد غذایی، دانشکده دامپزشکی، علوم پزشکی تبریز، دانشگاه آزاد اسلامی، تبریز، ایران.
3 - دانشیار گروه زیست‌شناسی جانوری، دانشکده علوم طبیعی، دانشگاه تبریز، تبریز، ایران.
4 - دانشیار گروه بهداشت مواد غذایی، دانشکده دامپزشکی، علوم پزشکی تبریز، دانشگاه آزاد اسلامی، تبریز، ایران.
5 - دانشیار گروه علوم و صنایع غذایی، دانشکده کشاورزی، واحد تبریز، دانشگاه آزاد اسلامی، تبریز، ایران.
تاریخ دریافت : 1401/05/05
تاریخ پذیرش : 1401/09/09
تاریخ انتشار : 1401/09/01
کلید واژه:
PCR,
کبد,
سکوم,
جوجه‌گوشتی,
هلیکوباکتر پلوروم,
چکیده مقاله :
نقش بالقوه هلیکوباکتر پلوروم به عنوان عامل نوظهور ایجاد عفونت غذایی و یک بیماری مشترک بین انسان و دام ثابت شده است. این باکتری از محتویات سکوم، کبد و دوازدهء طیور تخم‌گذار و گوشتی و همچنین از محتویات دستگاه گوارش و مجاری صفراوی در موارد بیماری‌های التهاب معده ای – روده‌ای انسان، جدا شده است. هدف از انجام مطالعه حاضر، بررسی آلودگی به هلیکوباکتر پلوروم در طیور گوشتی دارای علائم آنتریت ارجاعی به یکی از کلینیک‌های طیور سطح شهر تبریز بود. بدین منظور، تعداد 70 نمونه محتویات سکوم و 70 نمونه کبد مربوط به 14 گله جوجه گوشتی با استفاده از روش‌های کشت باکتریایی و PCR (Polymerase Chain Reaction) مورد ارزیابی قرارگرفت. بر اساس یافته ها، تعداد10 نمونه از محتویات سکوم مربوط به 2 گله متفاوت، در آزمایشات مربوط به کشت و نیز آزمون مولکولی PCR، از نظر وجود باکتری هلیکوباکتر پلوروم مثبت بودند، در حالی که هیچ یک از نمونه‌های کبد از این نظر مثبت نبود. با توجه به نقش هلیکوباکتر پلوروم در ایجاد تلفات وخسارت در طیور پرورشی و اهمیت اقتصادی مربوطه و جایگزینی آن در دستگاه گوارش طیور و امکان ایجاد آلودگی ثانویهء لاشه‌ها در حین عملیات کشتار و نیز زئونوز بودن بیماری مذکور ، لذا باکتری فوق به‌عنوان یک عامل خطر بهداشتی تهدیدکننده سلامت مصرف‌کنندگان، همواره مطرح می‌باشد.
چکیده انگلیسی:
The potential role of Helicobacter pullorum as an emerging cause of foodborne infection and a common disease between humans and livestock has been proven. This bacterium has been isolated from the contents of the cecum, liver and duodenum of laying and broiler poultry, as well as from the contents of the digestive system and bile ducts in human cases of gastro-intestinal inflammatory diseases. The aim of this study was to investigate Helicobacter pullorum infection in broiler poultry with symptoms of enteritis referred to one of the poultry clinics in Tabriz city. For this purpose, 70 samples of cecal contents and 70 liver samples from 14 broiler flocks were evaluated using bacterial culture and PCR (Polymerase Chain Reaction) methods. Based on the findings, 10 cecal content samples from 2 different herds were positive for the presence of Helicobacter pullorum bacteria in culture and molecular PCR tests, while none of the liver samples were positive in this respect. Considering the role of Helicobacter pullorum in causing mortality and damages in poultry farms and its related economic importance and the possibility of causing secondary carcass contamination during the slaughter due to presence of the bacterium in the digestive system of poultry as well as the zoonotic nature of the disease, therefore the above bacterium should always be considered as a health risk factor threatening the health of consumers.
منابع و مأخذ:
Akhlaghi, H., Emadi chashmi, S.H. and Jebeli javan, A. (2021). Development of a noval and specialized cultivation method for isolating Helicobacter pullorum from chichken meat. Iranian journal of veterinary research, Shiraz University, 74(1): 76-80.
Atabay, H.I., Corry, J.E.L. and On, S.L.W. (1998). Identification of unusual Campylobacter- Like isolates from poultry products as Helicobacter pullorum. Journal of Applied Microbiology, 84(6): 1017-1024.
Basharan Kahraman, B., Metiner, K., DirenSigirci, B., Chelik, B., Adiguzel, M.C., Bagcigil, A.F., et al. (2016). The effect of different storage temperatures and times on the viability of Helicobacter pullorum. Kafkas University Veterinary Journal, 22(3): 457-459.
Behroo, sh., Javadi, A. and Rad, M.G. (2015). Helicobacter pullorum prevalence in patients with gastroenteritis in humans and chicken in the provience of Ardabil in 2014. Indian Journal of Fundamental Applied Life Science, 5(2): 87-94.
Borges, V., Santos, A., Correia, C.B., Saraiva, Menard, A., Vierira, L., Sampaio, D.A., et al. (2015). Helicobacter pullorum isolated from fresh chicken meat. Antibiotic resistance and genomic traits of an emerging foodborne pathogene. Applied and Envronmental Microbiology, 81(23): 8155-8163.
Burnens, A.P., Stanly, J. and Nicolet, J. (1996) Campylobacters, Helicobacters and Related Organisms. New York: Springers Sciences and Businness Media, pp: 291-293.
Ceelen, L.M., Decostere, A., Verschaegen, G., Ducatelle, R. and Haesebrouc, K.F. (2005). Prevalance of Helicobacter pullorum among Patients with Gastrointestinal diseases and clinically healthy persons. Journal of Clinical Microbiology, 43(6): 2984-2986.
Ceelen, L.M., Decostere, A., Van Den Bulck, K., On, S.L.W., Baele, M., Ducatelle, R., et al. (2006). Helicobacter pullorum in chickens, Belgium. Emerging Infectious Diseases, 12(2): 263-267.
Ceelen, L.M., Decostere, A., Chiers, K., Ducatelle, R., Maes, D. and Haesebrouck, F. (2007). Pathogenesis of Helicobacter pullorum infection in broilers. International Journal of Food Microbiology, 116(2): 207-213.
Fox, J.G., Dewhirst, F.E., Shen, Z., Feng, Y., Taylor, N.S., Paster, B.J., et al. (1998). Hepatic Helicobacter species identified in bile and gallbladder tissue from Chileans with chronic cholecystitis. Gastroenterology, 114(4): 775-763.
Galal Abdel Hameed, K. and Sender, G. (2011). Prevalence of Helicobacter pullorum in Egyptian hens eggs and in vitro susceptibility to different antimicrobial agents.Animal Science Papers and Reports, 29(3): 257-264.
Hamada, M., Elbehiry, A., Marzouk, E., Moussa, I.M., Hessain, A.M., Alhaji, J.H., et al. (2018). Helicobacter pylori in a poultry slaughterhouse: prevalence, genotyping and antibiotic resistance pattern. Saudi journal of biological science, 25(6): 1072-1078.
Hassan, A.K., Shahata, M.A., Refaie, E.M. and Ibrahim, R.S. (2014). Pathogenicity testing and antimicrobial susceptibility of Helicobacter pullorum isolates from chicken origin. International Journal of Veterinary Science and Medicine, 2(1): 72-77.
Jamshidi, A., Bassami, M.R., Salamati, H. and Mohammadi, S. (2014). Isolation and identification of Helicobacter pullorum from cecal content of broiler chickens in Mashhad, Iran. Iranian Journal of Veterinary Research, Shiraz University, 15(2): 179-182.
Javed, K., Gul, F., Abbasi, R., Zaidi, R.A., Noreen, Z., Bokhari, H., et al. (2019). Prevalence and role of type six secretion system in pathogenesis of emerging zoonotic pathogen Helicobacter pullorum from retail poultry. Avian Pathology, 48(6): 557-563.
Jebeli javan, A., Emadi chashmi, S.H., Staji, H. and Akhlaghi, H. (2020). Comparison of culture and PCR method in prevalence of Helicobacter pullorum isolated from chicken liver samples and determination of antibiotic resistance profile of the isolates. Journal of Veterinary Microbiology, 16(2): 73-83.
Mohamed, M., Ragab, I., Shahata, M.and EI-Refaie, M. (2010). Helicobacter pullorum among poultry in ASSIUT Egypt: Genetic Characterization,Virulence and MIC. International Journal of Poultry Science, 9(6): 521-526.
Parente, M.R., Monteiro, J.T., Martins, G.G. and Saraiva, L.M. (2016). Helicobacter pullorum induces nitric oxide release in murine macrophages that promotes phagocytosis and killing. Microbiology, 162(3): 503-512.
Pilon, C., Prouzel-Mauleon, V., Menard, A. and Megraud, F. (2005). Development of a real-time quantitative PCR specific to Helicobacter pullorum. Thirteenth international workshop on Campylobacter, Helicobacter and related organisms (CHRO). Abstracts of scientific Presentations, Sep 4-8th, Gold coast, Queensland, Australia, pp: 62.
Stanly, J., Linton, D., Burnens, A.P., Dewhirst, F.E., On, S.L.W., Porter, A., et al. (1994). Helicobacter pullorum sp.nov.-genotype and phenotype of a new species isolated from poultry and from human patients with gastroenteritis. Microbiology, 140(12): 3441-3449.
Steele, W.T. and McDermott, S.N. (1984). The use of membrane filters applied directly to the surface of agar plates for the isolation of campylobacter jejuni from feces. Pathology, 16(3): 263-265.
Steinbruckner, B., Haerter, G., Pelz, K., Weiner, S., Rump, G.A., Deissler, W., et al. (1997). Isolation of Helicobacter pullorum from patients with enteritis. Scandinavian Journal of Infectious Diseases, 29(3): 315-318.
Takano, M., Yasin, M. and Shibasaki, I. (1979). Bacteriological effects of freezing with chemical agents. Journal of Food Scince, 44(1): 112-115.
Zanoni, R.G., Rossi, M., Giacomucci, D., Sanguinetti, V. and Manfreda, G. (2007). Ocurrence and antibiotic susceptibility of Helicobacter pullorum from broiler chickens and commercial laying hens in Italy. International Journal of Food Microbiology, 116(1): 168-173.
Zanoni, R.G., Piva,S., Rossi, M., Pasquali, F., Lucchi, A., De Cesare, A., et al. (2011). Occurrence of Helicobacter pullorum in Turkeys. Veterinary Microbiology, 149(3-4): 492-496.
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Akhlaghi, H., Emadi chashmi, S.H. and Jebeli javan, A. (2021). Development of a noval and specialized cultivation method for isolating Helicobacter pullorum from chichken meat. Iranian journal of veterinary research, Shiraz University, 74(1): 76-80.
Atabay, H.I., Corry, J.E.L. and On, S.L.W. (1998). Identification of unusual Campylobacter- Like isolates from poultry products as Helicobacter pullorum. Journal of Applied Microbiology, 84(6): 1017-1024.
Basharan Kahraman, B., Metiner, K., DirenSigirci, B., Chelik, B., Adiguzel, M.C., Bagcigil, A.F., et al. (2016). The effect of different storage temperatures and times on the viability of Helicobacter pullorum. Kafkas University Veterinary Journal, 22(3): 457-459.
Behroo, sh., Javadi, A. and Rad, M.G. (2015). Helicobacter pullorum prevalence in patients with gastroenteritis in humans and chicken in the provience of Ardabil in 2014. Indian Journal of Fundamental Applied Life Science, 5(2): 87-94.
Borges, V., Santos, A., Correia, C.B., Saraiva, Menard, A., Vierira, L., Sampaio, D.A., et al. (2015). Helicobacter pullorum isolated from fresh chicken meat. Antibiotic resistance and genomic traits of an emerging foodborne pathogene. Applied and Envronmental Microbiology, 81(23): 8155-8163.
Burnens, A.P., Stanly, J. and Nicolet, J. (1996) Campylobacters, Helicobacters and Related Organisms. New York: Springers Sciences and Businness Media, pp: 291-293.
Ceelen, L.M., Decostere, A., Verschaegen, G., Ducatelle, R. and Haesebrouc, K.F. (2005). Prevalance of Helicobacter pullorum among Patients with Gastrointestinal diseases and clinically healthy persons. Journal of Clinical Microbiology, 43(6): 2984-2986.
Ceelen, L.M., Decostere, A., Van Den Bulck, K., On, S.L.W., Baele, M., Ducatelle, R., et al. (2006). Helicobacter pullorum in chickens, Belgium. Emerging Infectious Diseases, 12(2): 263-267.
Ceelen, L.M., Decostere, A., Chiers, K., Ducatelle, R., Maes, D. and Haesebrouck, F. (2007). Pathogenesis of Helicobacter pullorum infection in broilers. International Journal of Food Microbiology, 116(2): 207-213.
Fox, J.G., Dewhirst, F.E., Shen, Z., Feng, Y., Taylor, N.S., Paster, B.J., et al. (1998). Hepatic Helicobacter species identified in bile and gallbladder tissue from Chileans with chronic cholecystitis. Gastroenterology, 114(4): 775-763.
Galal Abdel Hameed, K. and Sender, G. (2011). Prevalence of Helicobacter pullorum in Egyptian hens eggs and in vitro susceptibility to different antimicrobial agents.Animal Science Papers and Reports, 29(3): 257-264.
Hamada, M., Elbehiry, A., Marzouk, E., Moussa, I.M., Hessain, A.M., Alhaji, J.H., et al. (2018). Helicobacter pylori in a poultry slaughterhouse: prevalence, genotyping and antibiotic resistance pattern. Saudi journal of biological science, 25(6): 1072-1078.
Hassan, A.K., Shahata, M.A., Refaie, E.M. and Ibrahim, R.S. (2014). Pathogenicity testing and antimicrobial susceptibility of Helicobacter pullorum isolates from chicken origin. International Journal of Veterinary Science and Medicine, 2(1): 72-77.
Jamshidi, A., Bassami, M.R., Salamati, H. and Mohammadi, S. (2014). Isolation and identification of Helicobacter pullorum from cecal content of broiler chickens in Mashhad, Iran. Iranian Journal of Veterinary Research, Shiraz University, 15(2): 179-182.
Javed, K., Gul, F., Abbasi, R., Zaidi, R.A., Noreen, Z., Bokhari, H., et al. (2019). Prevalence and role of type six secretion system in pathogenesis of emerging zoonotic pathogen Helicobacter pullorum from retail poultry. Avian Pathology, 48(6): 557-563.
Jebeli javan, A., Emadi chashmi, S.H., Staji, H. and Akhlaghi, H. (2020). Comparison of culture and PCR method in prevalence of Helicobacter pullorum isolated from chicken liver samples and determination of antibiotic resistance profile of the isolates. Journal of Veterinary Microbiology, 16(2): 73-83.
Mohamed, M., Ragab, I., Shahata, M.and EI-Refaie, M. (2010). Helicobacter pullorum among poultry in ASSIUT Egypt: Genetic Characterization,Virulence and MIC. International Journal of Poultry Science, 9(6): 521-526.
Parente, M.R., Monteiro, J.T., Martins, G.G. and Saraiva, L.M. (2016). Helicobacter pullorum induces nitric oxide release in murine macrophages that promotes phagocytosis and killing. Microbiology, 162(3): 503-512.
Pilon, C., Prouzel-Mauleon, V., Menard, A. and Megraud, F. (2005). Development of a real-time quantitative PCR specific to Helicobacter pullorum. Thirteenth international workshop on Campylobacter, Helicobacter and related organisms (CHRO). Abstracts of scientific Presentations, Sep 4-8th, Gold coast, Queensland, Australia, pp: 62.
Stanly, J., Linton, D., Burnens, A.P., Dewhirst, F.E., On, S.L.W., Porter, A., et al. (1994). Helicobacter pullorum sp.nov.-genotype and phenotype of a new species isolated from poultry and from human patients with gastroenteritis. Microbiology, 140(12): 3441-3449.
Steele, W.T. and McDermott, S.N. (1984). The use of membrane filters applied directly to the surface of agar plates for the isolation of campylobacter jejuni from feces. Pathology, 16(3): 263-265.
Steinbruckner, B., Haerter, G., Pelz, K., Weiner, S., Rump, G.A., Deissler, W., et al. (1997). Isolation of Helicobacter pullorum from patients with enteritis. Scandinavian Journal of Infectious Diseases, 29(3): 315-318.
Takano, M., Yasin, M. and Shibasaki, I. (1979). Bacteriological effects of freezing with chemical agents. Journal of Food Scince, 44(1): 112-115.
Zanoni, R.G., Rossi, M., Giacomucci, D., Sanguinetti, V. and Manfreda, G. (2007). Ocurrence and antibiotic susceptibility of Helicobacter pullorum from broiler chickens and commercial laying hens in Italy. International Journal of Food Microbiology, 116(1): 168-173.
Zanoni, R.G., Piva,S., Rossi, M., Pasquali, F., Lucchi, A., De Cesare, A., et al. (2011). Occurrence of Helicobacter pullorum in Turkeys. Veterinary Microbiology, 149(3-4): 492-496.
