مطالعه مقایسه¬ای خصوصیات کمّی و کیفی اسپرم و ویژگی‌های هیستومورفومتری بافت بیضه در خروسهای مولد سویه آرین با راس 308
محورهای موضوعی : آسیب شناسی درمانگاهی دامپزشکیمنوچهر عالی مهر عالی مهر 1 * , لقمان احمد زاده 2 , محمد حسین علوی 3 , علیرضا طالبی 4 , مزدک رازی 5
1 - دانشگاه ارومیه ، دانشکده دامپزشکی
2 - دانشگاه ارومیه ، دانشکده دامپزشکی
3 - دانشگاه ارومیه ، دانشکده دامپزشکی
4 - استاد بیماری های طیور دانشگاه ارومیه
5 - گروه بافت شناسی مقایسه ای، دانشکده دامپزشکی، دانشگاه ارومیه
کلید واژه: آرین, راس 308, خروس, اسپرم, بیضه ,
چکیده مقاله :
زمینه و هدف: باروری در پرورش مرغ مادر نقش برجسته ای را دارد و کاهش نطفه داری خروس های مولّد یکی از چالشهای مهّم پرورش مرغ مادر گوشتی میباشد. با عنایت به اینکه پرورش سویه آرین برای پرورش مرغ مادر در کشور ایران رایجتر شده است. لذا این پژوهش به بررسی مقایسهای شاخصهای اسپرمی (تعداد، زنده مانی و تحرک) و هیستومورفومتری بافت بیضه در سویه آرین با سویه راس 308 (رایجترینسویه جهان) صورت گرفت.
مواد و روش کار: در این مطالعه نمونه‌های اسپرم و بافت بیضه شصت خروس (۳۰ خروس آرین، ۳۰ خروس راس ۳۰۸) در سه گروه سنی (35، ۴۵ و ۵۵ هفتگی) مورد ارزیابی قرار گرفتند.
نتایج: نتایج این مطالعه بیانگر ان است که در شاخصهای اسپرمی، تعداد اسپرم سویه آرین در سنین35، 45 و 55 هفتگی بطور غیر معنی دار از تعداد اسپرم در سویه راس بیشتر بود. زنده مانی اسپرم در 35 هفتگی در راس و در سن 55 هفتگی در آرین بالاتر بود ولی در سن 45 هفتگی تفاوت جندانی مشاهده نگردید. تحرک اسپرم در سن 35 هفتگی در راس و در سنین 45 و 55 هفتگی در آرین بالاتر بود. مطالعه هیستومورفومتری بافت بیضه نشان داد که شاخصهای اسپرمیوژنز، تمایز لوله‌ای، سلول‌های سرتولی، میوزی و قطر لوله‌های منی‌ساز در بین این سویهها تفاوت معنی‌داری ندارند.
نتیجه گیری: پارامترهای ارزیابی اسپرم در خروسهای هر دو سویه با افزایش سن روند کاهشی را داشته و به طور کلی، باروری خروسهای سویه آرین در مقایسه با سویه راس 308، عملکرد برتر را در سنین بالاتر نشان داد.
Reduced fertility with advancing age is one of the main challenges in broiler breeder production. Since no comparative study has evaluated the fertility characteristics of the Arian strain alongside Ross 308, This study investigated sperm indices (motility, viability, and count) and testicular histomorphometry at 35, 45, and 55 weeks of age in both strains. Sperm and testicular samples were collected from 60 roosters (30 Arian, 30 Ross 308). The results of this study indicated that at 35 weeks, Arian had a higher (not significant) sperm count than Ross 308; at 45 and 55 weeks, no significant difference was observed, and at 55 weeks, Arian again exhibited a non-significant increase. In both strains, sperm count declined with age. At 35 weeks, sperm viability was higher in Ross 308, but at 45 weeks there was no significant difference; whereas at 55 weeks, Arian showed relatively higher viability. Sperm motility was higher in Ross 308 at 35 weeks but was greater in Arian at 45 and 55 weeks. Histological assessments revealed no significant differences between the strains regarding spermiogenesis, tubular differentiation, Sertoli cell counts, meiotic cells, or seminiferous tubule diameter. Sperm parameters in both strains showed a declining trend with age. Overall, Arian’s fertility was comparable to that of Ross 308 and demonstrated superior performance at older ages.
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