مطالعه اثرات محافظتی آستاگزانتین بر آسیب و اختلال عملکرد بیضه ناشی از استرس گرما در موش¬های صحرایی نژاد ویستار
محورهای موضوعی : آسیب شناسی درمانگاهی دامپزشکی
طاها باقری
1
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علی اصغر طهرانی
2
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یوسف دوستار
3
1 - دستیار پاتوبیولوژی، گروه پاتوبیولوژی، دانشکده دامپزشکی، دانشگاه ارومیه، ارومیه، ایران
2 - گروه پاتوبیولوژی، دانشکده دامپزشکی، دانشگاه ارومیه، ارومیه، ایران
3 - گروه پاتوبیولوژی، دانشکده دامپزشکی، علوم پزشکی تبریز، دانشگاه آزاد اسلامی، تبریز، ایران
کلید واژه: استرس گرما, بیضه, آستاگزانتین, موش صحرایی,
چکیده مقاله :
ناباروری یک اختلال پیچیده با جنبه‌های پزشکی و اقتصادی است. آستاگزانتین به عنوان یک کاروتنوئید طبیعی دارای خواص آنتیاکسیدانی محافظتی و ضدسرطانی است. هدف از اين مطالعه ارزيابي تاثير آستاگزانتین بر آسيب ناشي از استرس حرارتي در بيضه موش صحرایی بود. برای این منظور 40 سر موش نر نژاد ویستار بهطور تصادفي در 4 گروه 10 تایی شامل: شاهد، استرس گرما، استرس گرما بهعلاوه تیمار با دز پایین آستاگزانتین و استرس گرما بهعلاوه تیمار با دز بالای آستاگزانتین (0/5 و mg/kg/day 1) تقسیم شدند. بیضه موشها به مدت 20 دقیقه داخل حمام آب 42 درجه سلسیوس غوطهور گرديد. در گروه شاهد غوطهورسازی بیضهها در دمای 23 درجه سلسیوس انجام شد. نمونه خون براي سنجش میزان تستوسترون اخذ شد و موشها آسانکشی شدند. از بيضه موشها برای ارزیابی هیستوپاتولوژی، ارزیابی وضعیت اسپرماتوژنز و تعیین وضعيت اكسيداني-آنتياكسيداني نمونه برداری گردید. آستاگزانتین سطح تستوسترون خون را در موشهای تحت استرس گرما بهطور افزایش و میزان شاخص درجه استرس اکسیداتیو را بهبود بخشيد (01/0>p). مشاهدات هيستوپاتولوژيك تغییرات دژنراسيون پيشروندهاي را پس از استرس حرارتي در بافت بيضه نشان داد. در موشهاي تيمارشده با آستاگزانتین آسیب بيضهها کاهش یافت و تغییرات مورفولوژیک و روند اسپرماتوژنز در آن بهطور معنیداری بهبود پیدا کرد. نتايج نشان داد كه آستاگزانتین ميتواند روند اسپرماتوژنز و آسیب اکسیداتیو بیضه ناشی از استرس حرارتی را بهبود بخشد.
Infertility is a complex problem with medical and economic aspects. Astaxanthin, as a natural carotenoid, has protective antioxidant and anticancer properties. The aim of this study was to evaluate the effect of astaxanthin on the damage induced by heat stress in rat testis. For this purpose, 40 male Wistar rats were randomly divided into 4 equal groups including: control, heat stress, heat stress plus treatment with a low dose of astaxanthin, and heat stress plus treatment with high dose of astaxanthin (0/5 and 1 mg/kg/day). The scrotum of heat stress rats was immersed in a water bath at 42 Celsius for 20 minutes. In the control group, immerssion of the testicles were carried out in a temperature of 23 Celsius. Blood samples were taken to measure testosterone levels, then all rats were euthanized. The testicles of the rats were also removed for histopathology, evaluation of the state of spermatogenesis and determination of the oxidative-antioxidant status. Astaxanthin increased level of blood testosterone in the rats which underwent heat stress and improved the activity of oxidative stress index (p<0/01). Histopathological observations demonstrated progressive degenerative changes in the testicular tissue after thermal stress, while in the rats treated with astaxanthin, testicular damage was reduced and the morphologic changes as well as spermatogenesis improved significantly. The results of this study showed that the astaxanthin can protect the testicular tissue against damage induced by heat stress and improve the alterations in its spermatogenesis and oxidative-antioxidant status.
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