Coating of Iron Oxide Nanoparticles with Human and Bovine Serum Albumins: A Thermodynamic Approach

Subject Areas : Journal of Physical & Theoretical Chemistry

Received: 2015-05-31 Accepted : 2015-05-31 Published : 2011-08-01

Keywords: Iron oxide nanoparticles, Fe304, human serum albumin (ESA), bovine serum albumin&#x0D, &#x0D, (BSA),

Abstract :

In this research, the Magnetite nanoparticles (Fe304) were prepared by coprecipitation of Fe3+ andFE solution in alkaline medium. Two kinds of surfactants, cetyl tri methyl ammonium bromide(CTAB) and cetyl pyridinum chloride (CPC) were used in the synthesis. Fe304 Nanoparticles werecoated with human serum albumin (HSA) and bovine serum albumin (BSA). Characteristics ofcoated magnetic nanoparticles and no coated were carried out using scanning electron microscopy,X-ray diffraction and FT-IR spectroscopy. The interactions of colloidal iron oxide nanoparticles withserum albumins, including BSA and EISA, were investigated by UV-Vis spectroscopy at processdifferent pH and temperatures. The thermodynamic parameters of coating were obtained from Van'tHoof equation. The results revealed that both AM: and ASo of reactants positive values. These resultsalso demonstrate that p1-I can play an important role on adsorption of proteins on iron oxidenanoparticles.

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Coating of Iron Oxide Nanoparticles with Human and Bovine Serum Albumins: A Thermodynamic Approach