Synthesis of PbS Functionalized with gelatin as a Fluorescence-Enhanced Sensor for determination of Phenylpropanolamine (PPA) drug in blood and urine samples

Subject Areas : Journal of Physical & Theoretical Chemistry

Shirin Bouroumand ¹ , Farzaneh Marahel ² , Fereydoon Khazali ³

1 - Department of Chemistry, Omidiyeh Branch, Islamic Azad University, Omidiyeh, Iran
2 - Department of Chemistry, Omidiyeh Branch, Islamic Azad University, Omidiyeh, Iran.
3 - Department of Chemistry, Omidiyeh Branch, Islamic Azad University, Omidiyeh, Iran

Received: 2020-12-30 Accepted : 2021-02-10 Published : 2021-11-01

Keywords: Fluorescence, Sensor PbS with Gelatin Synthesis, Quantum Dots, Determination, Phenylpropanolamine (PPA) Drug,

Abstract :

Sensor synthesis synthesized PbS functionalized with gelatin quantum dots have been considered as novel drugs sensors due to their ultrafine size, photo-stability and excellent fluorescent properties. In this study, a facile method was developed for the preparation of fluorescent water-soluble PbS quantum dots with gelatin sensor. The fluorescence of the PbS quantum dots sensor functionalized with gelatin synthesis is selectively and sensitively enhanced by addition of the Phenylpropanolamine (PPA) drug-induced aggregation of sensor PbS with gelatin. This finding was further used to design a fluorometric method for the determination of (PPA) drug, The reaction is followed fluoremetrically by measuring the absorbance at 335 nm. 2.5×10-2 molL-1 PbS with gelatin sensor, calibration graph in the rang of 0.05 - 10.0 µg L-1 (PPA) drug. The absorbance is linear from 0.05 up to 100.0 µg L−1 in aqueous solution with repeatability (RSD) of 3.5% at a concentration of 10.0 µg L−1 and a detection limit of 2.2 µg L−1 by the fixed-time method of 60 sec. The relative standard deviation for 10.0 µg L-1 (PPA) drug is %95. The applicability of the method was demonstrated by the determination of the (PPA) drug in urine and blood samples

References:

• Brückner, I. Hackbarth, T. Meinertz, B. Schmelzle, H. Scholz, The positive inotropic effect of phenylephrine in the presence of propranolol. Increase in time to peak force and in relaxation time without increase in c-AMP, Naunyn-Schmiedeberg's archives of pharmacology. 303 (1978) 205-211.


• A. Flavahan, Phenylpropanolamine constricts mouse and human blood vessels by preferentially activating α2-adrenoceptors, J. Pharmacol. Exp. Ther. 313 (2005) 432-439.


• British Pharmacopoeia. The stationary office, electronic version, London. 305 (2013) 1227, 1099.


• L. Suryan, V. K. Bhusari, K. S. Rasal, S.R. Dhaneshwar, Simultaneous quantitation and validation of paracetamol, phenylpropanolamine hydrochloride and cetirizine hydrochloride by RP‑HPLC in bulk drug and formulation, Int. J. Pharm. Sci .Drug Res. 3 (2011) 303–308.


• E. Balint, G. Falkay, G.A. Balint, Khat-a controversial plant, Wien, Klin. Wochenschr. 121 (2009) 604-614.


• Azhagvuel, R. Sekar, Method development and validation for the simultaneous determination of cetirizine dihydrochloride, paracetamol, and phenylpropanolamine hydrochloride in tablets by capillary zone electrophoresis, J. Pharm. Biomed. Anal. 43 (2007) 873–878


• B. Zhang, Z. G. Shen, J. X. Wang, H. Zhao, J. F. Chen, J. Yun, Nanonization of megestrol acetate by liquid precipitation, Ind. Eng. Chem. Res. 48 (2009) 8493–8499.


• E. Shal, A. K. Attia, Adsorptive stripping voltammetric behavior and determination of zolmitriptan using differential pulse and square wave voltammetry, Anal. Bioanal. Electrochem. 5 (2013) 32–45.


• Ghalkhani, S. Shahrokhian, F. G. Bidkorbeh, Voltammetric studies of sumatriptan on the surface of pyrolytic graphite electrode modified with multi-walled carbon nanotubes decorated with silver nanoparticles, Talanta. 80 (2009) 31-38.


• Karakuş, I. Küçükgüzel, S. G. Küçükgüzel, Development and validation of a rapid RP‑HPLC method for the determination of cetirizine or fexofenadine with pseudoephedrine in binary pharmaceutical dosage forms, J. Pharm. Biomed. Anal. 46 (2008) 295–302.


• Sunil, K. Yogesh, K. Abhijeet, Simultaneous estimation of cetirizine hydrochloride, phenylpropanolamine hydrochloride and paracetamol by RP‑HPLC method, Int. J. Pharm. Life. Sci. 4 (2013) 3122–3132.


• Abbasia, M. I. Bhangera, M. Y. Khuhawar, Capillary gas chromatographic determination of phenylpropanolamine in pharmaceutical preparation, J. Pharm. Biomed. Anal. 41 (2006) 998–1001.


• N. Makhija, P. R. Vavia, Stability indicating HPTLC method for the simultaneous determination of pseudoephedrine and cetirizine in pharmaceutical formulations, J. Pharm. Biomed. Anal. 25 (2001) 663–667.


• Dalpiaz, N. Marchetti, A. Cavazzini, L. Pasti, S. Velaga, E. Gavini, S. Beggiato, L. Ferraro, Quantitative determination of zolmitriptan in rat blood and cerebrospinal fluid by reversed phase HPLC–ESI-MS/MS analysis: application to in vivo preclinical pharmacokinetic study, J. Chromatogr. B 901 (2012) 72–78.


• B. Wankhede, K. A. Lad, S. S. Chitlange, Development and validation of UV‑spectrophotometric methods for simultaneous estimation of cetirizine
  
  

hydrochloride and phenylephrine hydrochloride in tablets, Int. J. Pharm. Sci. Drug. Res. 4 (2012) 222–226.

• I. Walash, N. El‑Enany, S. Saad, A new spectrophotometric method for determination of phenylpropanolamine HCl in its pharmaceutical formulations via reaction with 2,3,5,6-tetrachloro-1,4-benzoquinone, Int. J. Biomed. Sci. 6 (2010) 150–157.


• Vinas, C. Lopez‑Erroz, F. J. Cerdan, Hernandez‑Cordoba, Determination of phenylpropanolamine and methoxamine using fow‑injection with fluorimetric detection, Talanta. 47 (1998) 455–462.


• Shahrouei, S. Elhami, E. Tahanpesar, highly Sensitive detection of Cefriaxone in water, Food, Pharmaceutical and biological samles based on gold nanoparticles in aqueous and micellar media. J. Spectrochim Acta A Mol Biomols Spectrosc. 203 (2018) 287.


• D. Tan, J. H. Yin, G. Pu, Y. Yuan, L. Meng, N. Xu, A simple polyethylenimine-salicylaldehyde fluorescence probe: Sensitive and selective detection of Zn²⁺ and Cd²⁺ in aqueous solution by adding S^2- ion, J. Chem. Phys. Lett. 666 (2016) 68–72.


• Dai, K. Yao, J. Fu, K. Xue, L. Yang, K. Xu, A novel 2-(hydroxymethyl)quinolin-8-ol-based selective and sensitive fluorescence probe for Cd²⁺ ion in water and living cells, Sens. Actuators. B. 251 (2017) 877–884.


• B. Huang, W. Gu, H. X. Huang, J.B. Wang, W.X. Shen, Y.Y. Lv, J. Shen, A porphyrin-based fluorescent probe for optical detection of toxic Cd²⁺ ion in aqueous solution and living cells, Dyes. Pigments. 143 (2017) 427 – 435.


• B. Brahim, N. B. H. Mohamed, M. Echabaane, M. Haouari, R.B. Chaabane, M. Negrerie, H.B. Ouada, Thioglycerol-functionalized CdSe quantum dots detecting cadmium ions, Sens. Actuators. B. 220 (2015) 1346 – 1353.


• Shang, S. Dong, G.N. Nienhaus, Ultra-small fluorescent metal nanoclusters: Synthesis and biological applications. Nano Today. 6 (2011) 401–418.


• Xu, K.S. Suslick, Water-soluble fluorescent silver nanoclusters, Adv. Mater. 22 (2010) 1078–1082.


• Lu, W. Chen, Sub-nanometre sized metal clusters: From synthetic challenges to the unique property discoveries, J. Chem. Soc. Rev. 41 (2012) 3594–3623.


• Venkatesh, A. Shukla, S. Sivakumar, S. Verma, Purine-stabilized green fluorescent gold nanoclusters for cell nuclei imaging applications, ACS. Appl. Mater. Int. 6 (2014) 2185–2191.


• Knoblauch, M. Griep, C. Friedrich, Recent advances in the field of bionanotechnology: An insight into optoelectric bacteriorhodopsin, quantum dots, and noble metal nanoclusters, Sensors. 14 (2014) 19731–19766.


• Yang, H. Wang, K. Su, Y. Long, Z. Peng, N. Li, F. Liu, A facile and sensitive fluorescent sensor using electrospun nanofibrous film for nitroaromatic explosive detection, J. Mater. Chem. 21 (2011) 11895–11900.


• C. Chan, S. Nie, Quantum dot bioconjugates for ultrasensitive nonisotopic detection, Science. 281 (1998) 2016 – 2018.


• Shokrollahi, H.E. Haghighi, E. Niknam, and K. Niknam, Application of cloud point preconcentration and flame atomic absorption spectrometry for the determination of cadmium and zinc ions in urine, blood serum and water samples, Quim. Nova. Sao. Paul. 36 (2013) 273-282.


• Zeighami, S. Bagheri, N. Shadmani, Application of Flotation and Spectrophotometric Detection for Preconcentration and Separation of Trace Amounts of Cadmium Ion Using a New Ligand 3-((1H indole-3-yle) (4-Cyano Phenyl) Methyl) 1H Indole (ICPMI) in Real Samples. Hacettepe. J. Biol. Chem. 45 (2017) 277.


• Nadji, L. Nouri, A. Boudjemaa, D. Messadi, Predicting retention indices of PAHs in reversed-phase liquid
  
  

chromatography: A quantitative structure retention relationship approach. J. Serbian Chem. Soc. 85 (2020) 1-8.  

• S. Justin Packia Jacob, F. A. Narayanan, Synthesis of silver nanoparticles using Piper longum leaf extracts and its cytotoxic activity against Hep-2 cell line Colloids and Surfaces B: Biointerfaces. 91 (2012) 212–214.


• Koneswaran, R. Narayanaswamy, CdS/ZnS core-shell quantum dots capped with mercaptoacetic acid as fluorescent probes for Hg(II) ions, Microchimica. Acta. 178 (2012) 171-178.


• Zhao, J. Zou, W. Shi, In situ synthesis and characterization of lead sulfide nanocrystallites in the modified hyperbranched polyester by gamma-ray irradiation, Mater. Sci. Eng.121 (2005) 20-24.


• Baluta, A. Swist, J. Cabaj, K. Malecha, Point-of-Care Testing – Biosensor for Norepinephrine Determination. Int. J. Electronics. Telecom. 66 (2020) 369-372.


• S. Shyju, S. Anandhi, R. Sivakumar, R. Gopalakrishnan, Studies on Lead Sulfide (PbS) Semiconducting Thin Films Deposited from Nanoparticles and Its NLO Application, Int. J. Nanoscience. 13 (2014) 1450001-1450012.


• Krishnaraj, E. G. Jagan, S. Jagan, P. Rajasekar, P. T. Selvakumar, N. Kalaichelvan, Synthesis of silver nanoparticles using Acalypha indica leaf extracts and its antibacterial activity against water borne pathogens, Colloids and Surfaces. B: Biointerfaces. 76 (2010) 50–56.


• Pargari, F. Marahel, B. Mombini Godajdar. Design and Evaluation and Synthesis a Starch-Capped Silver NanoParticles Sensor and Determination trace Sulfacetamide Drug in the Presence Sodium borohydride in Blood and Urine Samples with Kinetic Spectrophotometric Method. J. Phys. Theor. Chem. 17 (2020) 1-14.


• Keskin, S. Allahverdiyeva, E. Şeyho, Y. Yardim, Determination of tramadol in pharmaceutical forms and urine samples using a boron-doped diamond electrode. J. Serb. Chem. Soc. 84


• S. Liang, L. Qi, R.L. Zhang, M. Jin, Z.Q. Zhang, Ratiometric fluorescence biosensor based on CdTe quantum and carbon dots for double strand DNA detection, Sens. Actuators. B. 244 (2017) 585–590.


• Dutta, D. Saikia, N. C. Adhikary, N.S. Sarma, Macromolecular Systems with MSA-Capped CdTe and CdTe/ZnS Core/Shell Quantum Dots as Superselective and Ultrasensitive Optical Sensors for Picric Acid Explosive, ACS. Appl. Mater. Interfaces. 7 (2015) 24778 – 24789.


• N. Prashanth, K. Basavaiah, M. S. Raghu, Spectrophotometric determination of zolmitriptan in bulk drug and pharmaceuticals using vanillin as a reagent, ISRN. Anal. Chem. (2013) 1–7.


• Xie, F. Zeng, S. Wu, Ratiometric fluorescent biosensor for hyaluronidase with hyaluronan as both nanoparticle scaffold and substrate for enzymatic reaction, Biomacromolecules. 15 (2014) 3383–3389.

S. Baluta, K. Malecha, A. Swist, J. Cabaj, Fluorescence Sensing Platforms for Epinephrine Detection Based on Low Temperature Cofired Ceramics. Sensors. 20 (2020) 1429.