اثر هشت هفته تمرین تناوبی شدید و تداومی با شدت متوسط همراه با عصاره بهار نارنج بر آلکالین فسفاتاز استخوانی و استئوکلسین در بافت فمور موش های صحرایی ماده سالمند
محورهای موضوعی : تاثیر حرکت و تمرین در سلامتی
فاطمه فرخایی
1
,
سعید کشاورز
2
,
الهام افتخاری
3
,
سید علی حسینی
4
1 - گروه فیزیولوژی ورزشی، واحد نجف آباد، دانشگاه آزاد اسلامی واحد نجف آباد، اصفهان، ایران
2 - دانشگاه آزاد نجف آباد
3 - مرکز تحقیقات پزشکی ورزشی، دانشگاه آزاد اسلامی، اصفهان، ایران
4 - گروه مدیریت ورزش، واحد مرودشت، دانشگاه آزاد اسلامی، مرودشت، ایران.
کلید واژه: تمرین, بهار نارنج, آلکالین فسفاتاز استخوانی, استئوکلسین, استخوان, سالمندی,
چکیده مقاله :
زمینه و هدف: پوکی استخوان در سالمندی از شایع ترین بیماری های متابولیکی استخوان است. از این رو هدف مطالعه حاضر بررسی اثر هشت هفته تمرین تناوبی شدید (HIIT) و تداومی با شدت متوسط (MICT) همراه با عصاره بهار نارنج (CA) بر آلکالین فسفاتاز استخوانی (BALP) و استئوکلسین (OCN) در بافت فمور موش¬های صحرایی ماده سالمند بود. مواد و روش¬ها: در این مطالعه تجربی 42 سر موش صحرایی ماده سالمند به طور تصادفی به گروه¬های (1) کنترل (C) (2) MICT، (3) HIIT، (4) MICT+CA، (5) HIIT+CA، (6) CA و (7) گروه شم (نرمال¬سالین) (Sham) تقسیم شدند. تمرینات HIIT با شدت 85% تا 110% VO2max و تمرینات MICT با شدت 65% VO2max انجام شد؛ مصرف بهار نارنج به میزان mg/kg/day 300 بود. نتایج: در گروه¬های CA، MICT، HIIT HIIT+CA و MICT+CA مقادیر BALP و OCN استخوانی بیشتر از گروه C بود (001/0=P). همچنین BALP در گروه MICT+CA بیشتر از گروه¬های CA، HIIT، MICT و HIIT+CA بود. مقادیر OCN در گروه MICT بیشتر از گروه HIIT بود (001/0=P). اما در گروه HIIT و MICT بیشتر از گروه¬های HIIT+CA و MICT+CA بود (001/0=P). نتیجه¬گیری: به نظر می¬رسد هرچند تمرین HIIT، MICT و CA هم به تنهایی و هم به طور تعاملی در بهبود متابولیسم استخوان موثر هستند، اما اثر تمرینات ورزشی به مراتب قوی¬تر از اثرات CA بر استئوپروز است.
Background and Purpose: Osteoporosis in old age is one of the most common metabolic bone diseases. Therefore, the aim of this study is to investigate the effect of eight weeks of high intensity interval training (HIIT) and moderate intensity continuous training (MICT) along with Citrus Aurantium (CA) on bone alkaline phosphatase (BALP) and osteocalcin (OCN) in the femur tissue of elderly female rats. Material and Methods: In this experimental study, 42 elderly female rats were randomly divided into 7 groups including 1) control (C), 2) MICT, 3) HIIT, 4) MICT+CA, 5) HIIT+ CA, 6) CA and 7) Sham (normal-saline). HIIT trainings were performed with an intensity of 85- 110% VO2max and MICT trainings were performed with an intensity of 65% VO2max; CA was consumed 300 mg/kg/day. Results: In the CA, MICT, HIITHIIT+CA and MICT+CA groups, BALP and OCN levels were higher than C group (P=0.001). In addition, BALP levels in MICT+CA group were higher than CA, HIIT, MICT and HIIT+CA groups. OCN levels in the MICT group were higher than the HIIT group (P=0.001). While in the HIIT and MICT group were higher than the HIIT+CA and MICT+CA groups (P=0.001). Conclusion: It seems that although HIIT, MICT and CA are effective (both alone and interactively) in improving bone metabolism, the effect of training is much stronger than the effects of CA on osteoporosis.
1. Imran M, Singh A, Bhardwaj A, Agrawal D. Prevalence of osteoporosis and associated risk factors among postmenopausal women: A cross-sectional study from Northern India. J Midlife Health. 2022;13(3):206.
2. Chandran M, Brind’Amour K, Fujiwara S, Ha Y-C, Tang H, Hwang J-S, et al. Prevalence of osteoporosis and incidence of related fractures in developed economies in the Asia Pacific region: A systematic review. Osteoporos Int. 2023;1–17.
3. Khajehlandi A, Mohammadi A. Interactive effect of aerobic training and estrogen consumption on serum levels of catalase and glutathione peroxidase enzymes in. Jorjani Biomed J. 2020;8(2):38–47.
4. Wang H, Luo Y, Wang H, Li F, Yu F, Ye L. Mechanistic advances in osteoporosis and anti‐osteoporosis therapies. MedComm. 2023;4(3):e244.
5. Hosseini SA, Salehi O, Keikhosravi F, Hassanpour G, Ardakani HD, Farkhaie F, et al. Mental health benefits of exercise and genistein in elderly rats. Exp Aging Res. 2022;48(1):42–57.
6. Alizadeh R, Salehi O, Rezaeinezhad N, Hosseini SA. The effect of high intensity interval training with genistein supplementation on mitochondrial function in the heart tissue of elderly rats. Exp Gerontol. 2023;171:112039.
7. Mohebbi R, Shojaa M, Kohl M, von Stengel S, Jakob F, Kerschan-Schindl K, et al. Exercise training and bone mineral density in postmenopausal women: An updated systematic review and meta-analysis of intervention studies with emphasis on potential moderators. Osteoporos Int. 2023;1–34.
8. Bakhtiyari M, Fathi M, Hejazi K. Effect of Eight Weeks of Aerobic Interval Training on the Serum Concentrations of Alkaline Phosphatase, Osteocalcin and Parathyroid Hormone in Middle-aged Men. Gene, Cell Tissue. 2021;8(3).
9. Manaye S, Cheran K, Murthy C, Bornemann EA, Kamma HK, Alabbas M, et al. The Role of High-intensity and High-impact Exercises in Improving Bone Health in Postmenopausal Women: A Systematic Review. Cureus. 2023;15(2).
10. Polisel EEC, Beck WR, Scariot PPM, Pejon TMM, Gobatto CA, Manchado-Gobatto FB. Effects of high-intensity interval training in more or less active mice on biomechanical, biophysical and biochemical bone parameters. Sci Rep. 2021;11(1):6414.
11. Kaikhosravi F, Daryanoosh F, Koushkie Jahromi M, Nemati J. The Effect of High Intensity Interval Training with Genistein on Biomechanical Properties of Femur Bone in Elderly Female Rats. مجله زیست پزشکی جرجانی. 2020;8(1):51–9.
12. Salehi O, Farkhaie F, Jamali Fashi R, Rakhshanizadeh A. The Effect of Interval and Continued Trainings with Citrus Aurantium on Pain Threshold and Motor Balance in Elderly Rats. Jorjani Biomed J. 2022;10(2):1–9.
13. Deshmukh NS, Stohs SJ, Magar CC, Kale A, Sowmya B. Bitter orange (Citrus aurantium L.) extract subchronic 90-day safety study in rats. Toxicol Reports. 2017;4:598–613.
14. He D, Liu Z, Wang M, Shu Y, Zhao S, Song Z, et al. Synergistic enhancement and hepatoprotective effect of combination of total phenolic extracts of Citrus aurantium L. and methotrexate for treatment of rheumatoid arthritis. Phyther Res. 2019;33(4):1122–33.
15. Di Pompo G, Kusuzaki K, Ponzetti M, Leone VF, Baldini N, Avnet S. Radiodynamic Therapy with Acridine Orange Is an Effective Treatment for Bone Metastases. Biomedicines. 2022;10(8):1904.
16. Hosseinpour Delavar S, Safikhani H, Azizi M. The Effect of Eight Weeks of Continuous and Interval Training with Citrus Aurantium Consumption on Autophagy Markers and MyoD Activation in the Muscle Tissue of Elderly Rats. Elder Heal J. 2021;7(2):71–8.
17. Shykholeslami Z, Abdi A, Barari A, Hosseini SA. The effect of aerobic training with Citrus aurantium L. on SIRT1 and PGC-1α gene expression levels in the liver tissue of elderly rats. Jorjani Biomed J. 2019;7(4):57–65.
18. He W, Li Y, Liu M, Yu H, Chen Q, Chen Y, et al. Citrus aurantium L. and its flavonoids regulate TNBS-induced inflammatory bowel disease through anti-inflammation and suppressing isolated jejunum contraction. Int J Mol Sci. 2018;19(10):3057.
19. Li F-H, Sun L, Zhu M, Li T, Gao H-E, Wu D-S, et al. Beneficial alterations in body composition, physical performance, oxidative stress, inflammatory markers, and adipocytokines induced by long-term high-intensity interval training in an aged rat model. Exp Gerontol. 2018;113:150–62.
20. Yazdanparast Chaharmahali B, Azarbayjani MA, Peeri M, Farzanegi Arkhazloo P. The Effect of Moderate and High Intensity Interval Trainings on Cardiac Apoptosis in the Old Female Rats. Rep Heal Care. 2018;4(1):26–35.
21. Keikhosravi F, Daryanoosh F, Koushkie Jahromi M, Nemati J. High-Intensity Interval Training Effects with Genistein on Serum Osteocalcin and Bone Alkaline Phosphatase in Female Elderly Rats. J Nutr Fasting Heal. 2021;9(2):125–30.
22. Shi Z, Wang L, Luan J, Yin L, Ji X, Zhang W, et al. Exercise promotes bone marrow microenvironment by inhibiting adipsin in diet-induced male obese mice. Nutrients. 2022;15(1):19.
23. Shalaby NMM, Abd-Alla HI, Ahmed HH, Basoudan N. Protective effect of Citrus sinensis and Citrus aurantifolia against osteoporosis and their phytochemical constituents. J Med Plants Res. 2011;5(4):579–88.