تأثیر هشت هفته تمرین شنا، سلول¬درمانی و لیزردرمانی بر بیان ژن¬های IL-2، IL-10 و STAT-3 بافت بیضه در موش¬های مدل آزواسپرمی
محورهای موضوعی : اثر فعالیت بدنی بر بیماری ها
سید ساسان حسینی
1
(
Department of Physical Education & sports sciences, Aliabad Katoul Branch, Islamic Azad University, Ali Abad Katool, Iran
)
Habib Asgharpour
2
(
Department of Exercise Physiology, Ali Abad Katool Branch, Islamic Azad University, Ali Abad Katool, Iran
)
پروین فرزانگی
3
(
عضو هیات علمی
)
کلید واژه: آزواسپرمی, تمرین شنا, سلول¬درمانی, لیزر درمانی و سیگنالینگ IL-10/ .STAT-3,
چکیده مقاله :
زمینه و هدف: آزواسپرمی به معنای کمبود اسپرم در مایع منی است. یک درصد از کل مردان و ده درصد از مردان نابارور مبتلا به آزواسپرمی هستند، هدف از پژوهش حاضر بررسی اثرات هشت هفته تمرین شنا، سلول¬درمانی و لیزردرمانی بر بیان ژن¬های IL-2، IL-10 و STAT-3 بافت بیضه در موش¬های مدل آزواسپرمی می¬باشد. روش کار: 40 سر رت 6 تا 8 هفته¬ای به¬صورت تصادفی انتخاب، و سپس مدل آزواسپرمی با داروی بوسولفان با دوز 40 میلی¬گرم در موش¬ها القاء شد. یک¬ماه بعد از ایجاد مدل یک¬بار سلول¬های بنیادی به¬صورت پیوند در ناحیه مجران دفران به میزان یک میلیون سلول پیوندزده شد، پس از گذشت یک هفته از پیوند سلول، لیزر با طول موج 8/632 نانومتر و توان 10 میلی¬وات و انرژی 3 ژول به¬صورت سه تکرار در کل دوره مطالعه با فاصله هر هفته یک¬بار اعمال شد. تمرین روزانه شنا به¬مدت 30 دقیقه و 5 روز در هفته در طول 8 هفته اجرا شد. یافته¬ها: نتایج نشان داد که القای مدل آزواسپرمی سبب افزایش معنی¬دار ژن¬های IL-2، IL-10 و STAT-3 نسبت به رت¬های گروه سالم شد (P≤0.05). هم¬چنین انجام روش¬های مداخله¬ای لیزر درمانی، سلول ¬درمانی و تمرین بیان این ژن¬ها در بافت بیضه رت¬ها را نسبت به گروه بیمار کاهش معنی¬داری داد (P≤0.05). نتیجه¬گیری: تمرین ورزشی شنا در ترکیب با سلول¬درمانی و لیزردرمانی از طریق کاهش سیگنالینگ التهاب بافت بیضه ممکن است به باروری رت¬های مدل آزواسپرمی کمک نماید.
purpose: Azoospermia means lack of sperm in semen. One percent of all men and ten percent of infertile men suffer from azoospermia, the purpose of this study is to investigate the effects of eight weeks of swimming training, cell and laser therapy on the expression of IL-2, IL-10 and STAT-3 genes in testicular tissue. It is azoospermia in rats. Methods: Forty 6- to 8-week-old rats were randomly selected, and then the azoospermia model was induced with Busulfan at a dose of 40 mg in mice. One month after induction of the model, stem cells were transplanted once in the vas deferens at the rate of one million cells per mouse. Then, one week after cell transplantation, a laser with a wavelength of 632.8 nm and a power of 10 mW and energy 3 joules were applied in three repetitions throughout the study period with an interval of once a week, and after improving the effects of surgery, swimming training were performed for 30 minutes and 5 days a week for 8 weeks. Results: The results showed that induction of azoospermia model caused a significant increase in IL-2, IL-10 and STAT-3 genes compared to healthy group rats (P≤0.05). Also, performing the interventional methods of laser therapy, cell therapy and exercise significantly reduced the expression of these genes in the testicular tissue of rats compared to the patient group (P≤0.05). Conclusion: Swimming exercise in combination with cell and laser therapy may help the fertility of azoospermia model rats by reducing testicular tissue inflammation signaling.
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