Eight weeks of high intensity interval training improve the expression of interleukin-17 and interleukin-18 genes in the heart tissue of cadmium-poisoned rats

Subject Areas : Journal of Physical Activity and Hormones

Ahmad  Ahmadloo ¹ (Ph.D candidate in Exercise Physiology, Department of Physical Education, Larestan branch, Islamic Azad University, Larestan, Iran)
Sirous  Farsi ² (Assistant Professor in Exercise Physiology, Department of Physical Education, Larestan branch, Islamic Azad University, Larestan, Iran)
Mohammad Ali  Azarbayjani ³ (Professor in Exercise Physiology, Department of Physical Education, Central Tehran branch, Islamic Azad University, Tehran, Iran)

Received: 2017-09-09 Accepted : 2017-12-18 Published : 2018-02-01

Keywords: Cadmium, HIIT training, IL-17, IL-18,

Abstract :

Introduction: lL-17 and IL-18 are pro-inflammatory cytokines. The aim of present study is to evaluate the effect of endurance training on IL-17 and IL-18 in heart tissue of rats which have been poisoned by cadmium. Material & Methods: Twenty Wistar rats were randomly divided into four groups: 1) control, 2) sham, 3) cadmium and 4) high intensity interval training (HIIT training) with cadmium. HIIT training was performed 8 weeks and 3 days a week (80 to 110% of their VO2max). Cadmium groups received 2mg/kg cadmium intraperitoneally everyday. At the end of protocol, the rats were sacrificed and their heart tissues were prepared to measure gene expression of IL-17 and IL-18. The data was analyzed by independent sample t-test and one-way ANOVA (P≥ 0.05). Results: The HIIT training led to significantly reducing IL-17 and IL-18 gene expression in heart issue of rats (P= 0.001). Conclusion: The 8 weeks of HIIT training will improve the levels of pro-inflammatory cytokines in rats which have been poisoned by cadmium.

References:

Referecences

1. Gurer H, Ercal N. Can antioxidants be beneficial in treatment of lead poisoning? Free Radic Biol Med 2000; 29: 927-945.

2. Simkhovich BZ, Kleinman MT, Kloner RA. Air Pollution and Cardiovascular Injury. Epidemiology, Toxicology, and Mechanisms. JAm Coll Cardiol 2008; 52: 719-726.

3. Ademuyiwa O, Agarwal R, Chandra R, Behari JR. Lead-induced phospholipidosis and cholesterolgenesis in rat tissues. Chem Biol Interact 2009; 179: 314-320.

4. Christopher JK, Hame tt, Prapavessis H, Chris Bald J, Varo N, Schoenbeck V, et al. Effects of exercise training on 5 inflammatory markers associated with cardiovascular risk. American heart J 2006; 151: 367-387.

5. Puri VN. Cadmium induced hypertension. Clin Exp Hypertens 1999; 21: 79-84.

6. Ding YH, Young CN, Luan X, Li J, Rafols JA, Clark JC, et al. Exercise preconditioning ameliorates inflammatory injury in ischemic rats during reperfusion. Acta neuropathol (Berl) 2005; 109: 237-46.

7. Laura E, Dick S, Paul L, Erik S. Exercise, cognition and Alzheimer’s disease: More is not necessarily better. Neurosci Biobehav Rev 2006; 30: 562-575.

8. Aggarwal BB, Harikumar KB. Potential therapeutic effects of curcumin, the antiinflammatory agent, against neurodegenerative, cardiovascular, pulmonary, metabolic, utoimmune and neoplastic diseases. Int J Biochem Cell Biol 2009; 41: 40-59.

9. Anthony Z, Eza L, Bagus PPW, Xin J, Komang S, Grace DS. High dietary turbine reduces apoptosis and atherosclerosis in the left main coronary artery; association with reduce CCAAT/enhancer binding protein homologous protein and total plasma homocysteine but not lipidemia. Hypertension 2009; 53: 1017-1022.

10. Barbara JMH, Jefferis A, Olia Papacosta A, Christopher G, Owen BS, Goya Wannamethee A, et al. Interleukin 18 and coronary heart disease: Prospective study and systematic review. Atherosclerosis 2011; 219:970

11. Moseley TA, Haudenschild DR, Rose L, Reddi AH. Interleukin-17 family and IL-17 receptors”. Cytokine Growth Factor Rev 2003; 14:155-74.

12. Jarup L, Akesson A. Current status of cadmium as an environmental health problem. Toxicol Appl Pharmacol 2009; 238: 201-208.

13. Tuomisto K, Jousilahti P, Sundvall J, Pajunen P, Salomaa V. C-reactive protein, interleukin-6 and tumor necrosis factor alpha as predictors of incidentcoronary and cardiovascular events and total mortality. A population-based, prospective study. Thromb Haemost 2006; 95: 511-518

14. Blair SN, Cheng Y, Holder JS. Is physical activity or physical fitness more important in defining health benefits? Med Sci Sports Exerc 2001; 33: 379-399

15. Dorneles GP, Haddad DO, Fagundes VO, Vargas BK, Kloecker A. High intensity interval exercise decreases IL-8 and enhances the immunomodulatory cytokine interleukin-10 in lean and overweight–obese individuals. Cytokine 2016; 77: 1-9

16. Yamaoka-Tojo M, Tojo T, Wakaume K. Circulating interleukin-18: a specific biomarker for atherosclerosis-prone patients with metabolic syndrome. Nutrition and Metabolism 2011; 8: 34-41.

17. Fichtlscherer S, Rosenberger G, Walter DH, Breuer S, Dimmeler S, Zeiher AM. Elevated Creactive protein levels and impaired endothelial vasoreactivity in patients with coronary artery disease. Circulation 2000; 102: 1000-1006.

18. Ravasi AA, Aminian T, Gaeini AA, Haghighi AH. Effect of continues training on proinflammatory cytokines and insulin resistance in obese male. Harekat 2005; 28: 57-66.

19. Ruiz J, Ortega F, Meusel D, Sjöström M. Traditional and novel cardiovascular risk factors in school-aged children: A call for the further developpment of public health strategies with emphasis on fitness. J Public Health 2007; 15: 171-177.

20. Gregory S, William F, Tom S. Correlation of hepatic metallothionein concentrations with acute cadmium toxicity in the mouse. Toxicol Appl Pharmacol 1977; 39: 61-69.

21. Thijssen S, Maringwa J, Faes C, Lambrichts I, Van Kerkhove E. Chronic exposure of mice to environmentally relevant, low doses of cadmium leads to early renal damage, not predicted by blood or urine cadmium levels. Toxicology 2007; 229: 145-156.

22. Agha-Alinejad H, Haftchenari SH, MatinHomaei H. Effect of a Period of Endurance Training on Serum Il-8 Concentration and Tumor Volume in Breast Cancer BearingMice. Iranian Journal of Endocrinology and Metabolism 2014; 16: 28-32.

23. Bedford T, Tipton C, Wilson N, Oppliger R, Gisolfi C. Maximum oxygen consumption of rats and its changes with various experimental procedures. Appl Physiol 1979; 47: 1278-1283.

24. Subramanyam G, Bhaskar M, Govindappa S. The role of cadmium in induction of atherosclerosis in rabbits. Indian Heart J 1992; 44: 177-180.

25. Mlynek V, Skoczyńska A. The proinflammatory activity of cadmium. Postepy Hig Med Dosw 2005; 59: 1-8.

26. Yamaoka-Tojo M, Tojo T, Wakaume K. Circulating interleukin-18: a specific biomarker for atherosclerosis-prone patients with metabolic syndrome. Nutr Metab 2011;8: 45-52.

27. Robson-Ansley PJ, Blannin A, Gleeson M. Elevated plasma interleukin-6 levels in trained male triathletes following an acute period of intense interval training. Eur J Appl Physiol 2007; 99: 353-360.

28. Tofighee A, Khazaei H, Jalili A. Comparison of Effect of One Course of Intense Exercise (Wingate test) on Serum Levels of Interleukin-17 in Different Groups of Athletes. Asian J Sports Med 2014; 5: e22769.

29. Duzova H, Karakoc Y, Hanifi MT, Yilmaz ZD, Kilinc E. Effects of acute moderate and strenuous exercise bouts on IL-17 production and inflammatory response in trained rats. J Sport Sci Med 2009; 8: 219-224.

30. Daryanoosh F, Jafari H, Rahimi E, Mehrbani D, Soltani F. The effect of eight week interval acute training on plasma visfatin, TNF-α and IL-6 in rats: a brief report. Tehran Univ Med J. 2013; 71 :603-608.

31. Mirdar S, Arab A, Hedayati M, Hajizadeh A. The effect of a course of swimming training program on the levels of lung hypoxia induced invasion of newborns in pregnant rats. Qom Univ Med Sci J 2013; 7: 11-20.

32. Leggate M, Carter WG, Evans JC, Vennard RA, Nimmo MA. Determination of inflammatory and prominent proteomic changes in plasma and adipose tissue after high-intensity intermittent training in overweight and obese males. J Appl Physiol 2012; 112: 1353-1360.

33. Nikseresht M, Agha-Alinejad H Azarbayjani M A, Ebrahim K. Effects of Nonlinear Resistance and Aerobic Interval Training on Cytokines and Insulin Resistance in Sedentary Men Who Are Obese. J Strength Condition Res 2014; 28: 2560-2568.

34. Steckling FM, Farinha JB, Santos DL, Bresciani G, Mortari JA, Stefanello ST, et al. High intensity interval training reduces the levels of serum inflammatory cytokine on women with metabolic syndrome. Exp Clin Endocrinol Diabetes 2016; 124: 597-601.

35. Leggate M, Carter WG, Evans JC, Vennard RA, Nimmo MA. Determination of inflammatory and prominent proteomic changes in plasma and adipose tissue after high-intensity intermittent training in overweight and obese males. J Appl Physiol 2012; 112: 1353-1360.

36. Rosendal L, Sogaard K, Kjaer M, Sjogaard G, Langberg H, Kristiansen J. Increase in interstitial interleukin-6 of human skeletal muscle with repetitive low-force exercise. J Appl Physiol 2005; 98: 477-481.

37. Gibala MJ, Little JP, van Essen M. Short-term sprint interval versus traditional endurance training: similar initial adaptations in human skeletal muscle and exercise performance. J Physiol 2006, 575: 901-911.