Evaluating the effect of low-intensity eccentric resistance training combined with blood flow restriction on the systematic and genetic indices affecting the activation and proliferation of satellite cells in young non-athlete men

Subject Areas : Journal of Physical Activity and Hormones

Azam  Mousavian ¹ (PhD Student in Exercise Physiology, Kish international campus, University of Tehran, Iran)
Abbas  Ali Gaeni ² (Department of Exercise Physiology, Faculty of Physical Education and Sport Science, University of Tehran, Tehran, Iran.)
Reza  Nuri ³ (Department of Exercise Physiology, Kish international campus, University of Tehran, Iran)
Mohammad Reza  Kordi ⁴ (Department of Exercise Physiology, Faculty of Physical Education and Sport Science, University of Tehran, Tehran, Iran)

Received: 2017-06-26 Accepted : 2017-08-10 Published : 2017-09-01

Keywords: Low-intensity eccentric resistance exercise training, Blood flow restriction, Systematic indices, Genetic indices, Satellite cells,

Abstract :

Introduction: The current study was conducted to evaluate the impact of low-intensity eccentric resistance training combined with and without blood flow restriction (ECCRT with BFR vs. ECCRT without BFR) on some of the systematic and genetic indices affecting the activation and proliferation of satellite cells in young non-athlete men. Materials and Methods: Twenty men with an age range of 25 ± 5 years were randomly divided into two groups of ECCRT with BFR and ECCRT without BFR (each group containing 10 subjects). ECCRT was performed using isokinetic device. It involves about 70 rpt in knee extensor muscles. Restriction was created using an air pressure meter. After local anesthesia through injecting lidocaine 1% and norepinephrine, sampling was performed from lateral broad muscle 48 hours before and 24 hours after the training, and the systematic and genetic indices affecting the activation and proliferation of satellite cells (HGF and Myf5) were examined and compared in two stages of pre-test and post-test in two situations of ECCRT with BFR vs. ECCRT without BFR. Results: The results of this study showed that HGF and Myf5 increased after ECCRT with BFR and ECCRT without BFR (P<0.05) and the increase of HGF and Myf5 was higher after ECCRT with BFR than after ECCRT without BFR (P<0.05). Conclusions: It is recommended for authorities and officials in the sports field to use low-intensity ECCRT with BFR to increase HGF and Myof5.

References:

1. Gholamali M, Shabani M, Hedayati M. Investigating the response of some of the most important proteins involved in cachexsia syndrome to acute resistance activity in healthy elderly people. Salmand 2015; 9: 288-296.

2. Jamal Pour Z, Asghari A, Nourani MR. Skeletal muscle tissue engineering: Present and future. Iranian J Militar Med 2012; 14: 77-84.

3. Takarada Y, Takazawa H, Sato Y, Takebayashi S, Tanaka Y, Ishii N. Effects of resistance exercise combined with moderate vascular occlusion on muscular function in humans. J Appl Physiol 2000; 88: 2097-2106.

4. Takarada Y, Sato Y, Ishii N. Effects of resistance exercise combined with vascular occlusion on muscle function in athletes. J Appl Physiol 2002; 86: 308-314.

5. Namju Garmi Z, Heydari MH, Norouzian M, Mastri Farahani R, Al-Tarihi T, et al. Investigating the process of differentiation of skeletal muscle cell-like cells derived from human adipose tissue mesenchymal stem cells affected by laboratory conditions. J North Khorasan University Med Sci 2013; 5: 147-157.

6. Allen RE, Sheehan SM, Taylor RG, Kendall TL, Rice GM. Hepatocyte growth factor activates quiescent skeletal muscle satellite cells in vitro . J Cell Physiol 1995; 165: 307-312.

7. Gal-Levi R, Leshem Y, Aoki S, Nakamura T, Halevy O. Hepatocyte growth factor plays a dual role in regulating skeletal muscle satellite cell proliferation and differentiation. Biochim Biophys Acta 1998; 1402: 39-51.

8. Crameri RM, Langberg H, Magnusson P, Jensen CH, Schrøder HD, Olesen JL, et al. Changes in satellite cells in human skeletal muscle after a single bout of high intensity exercise. J Physiol 2004; 558: 333-340.

9. Nielsen JL, Aagaard P, Bech RD, Nygaard T, Hvid LG, Wernbom M, et al. Proliferation of myogenic stem cells in human skeletal  muscle in response to low-load resistance training with blood flow restriction. J Physiol 2012; 590: 4351-4361.

10. Sheehan SM, Tatsumi R, Temm-Grove CJ, Allen RE. HGF is an autocrine growth factor for skeletal muscle satellite cells in vitro. Muscle Nerve 2000; 23: 239-245.

11. Tatsumi, Ryuichi ; &  Allen,  Ronald E ; (2004). "Active hepatocyte growth factor is present in skeletal muscle extracellular matrix. Muscle Nerve 30: PP: 654–658.

12. Behzad B and Asghari AR. The interactive role of exercise and satellite cells in the restoration and skeletal muscle hypertrophy. Ebnesina 2015; 16:47-63.

13. Yin H, Price F, Rudnicki MA. Satellite cells and the muscle stem cell niche. J Physiol Rev 93: 23-67.

14. Shadrach JL and Wagers AJ. Stem cells for skeletal muscle repair. Philos Trans R Soc Lond B Biol Sci 2011; 366: 2297-2306.

15. Tatsumi R, Anderson JE, Nevoret CJ, Halevy O, Allen RE. HGF/SF is present in normal adult skeletal muscle and is capable of activating satellite cells. Dev Biol 1998; 194: 114-128.