بررسی اثر عصاره‌ی جینسنگ (پاناکس جینسنگ) بر ساختار بافتی پوست در قزل‌آلای رنگین‌کمان (Oncorhynchus mykiss): مطالعه‌ی هیستومورفومتریک و بیوشیمیایی
محورهای موضوعی : نشریه فن آوریهای نوین در توسعه آبزی پروریعلی پرچمی 1 * , وحید پویاپور 2
1 - گروه علوم پایه - دانشکده‌ی دام‌پزشکی - دانشگاه شهرکرد - شهرکرد - ایران
2 - گروه علوم پایه دانشکده‌ی دام‌پزشکی، دانشگاه شهرکرد
کلید واژه: پوست, عصاره‌ی جینسنگ, قزل‌آلای رنگین‌کمان, هیستوشیمی, هیستومورفومتری,
چکیده مقاله :
هدف از پژوهش حاضر، ارزیابی اثر عصاره‌ی ریشه‌ی گیاه جینسنگ (پاناکس جینسنگ) بر ویژگی‌های هیستومورفومتریک و بیوشیمیایی پوست در قزل‌آلای رنگین‌کمان بود. پژوهش در بازه‌ی زمانی ۶۰ روزه در ۴ تیمار آزمایشی با سه تکرار صورت گرفت. در هر تکرار، به‌صورت اتفاقی ۶۰ قطعه بچه‌ماهی با میانگین وزنی ۱۵ گرم توزیع شد. گروه‌های آزمایشی به‌ترتیب با ۰، ۱۰۰، ۱۵۰ و ۲۰۰ گرم در کیلوگرم جیره، عصاره‌ی آبی جینسنگ تغذیه شدند. نتایج حاصل از مطالعه‌ی بافتی پوست نواحی پشتی و شکمی بدن نشان داد که میانگین ضخامت هیچ‌یک از لایه‌های پوستی اپیدرم، درم و هیپودرم در گروه‌های دریافت‌کننده‌ی جینسنگ تفاوت معناداری با گروه شاهد نشان نمی‌دهد (p>0.05). میانگین شمار تام سلول‌های موکویید و میانگین شمار کروماتوفورها در همه‌ی گروه‌های مورد مطالعه به‌نحو معناداری در پوست ناحیه‌ی پشتی بیش‌تر از پوست ناحیه‌ی شکمی بوده (p0.05). با افزایش میزان جینسنگ، میانگین شمار سلول‌های موکویید در پوست هر دو ناحیه‌ی پشتی و شکمی با الگویی خطی به‌نحو معناداری در مقایسه با گروه شاهد افزایش یافته (p0.05).
The aim of the present study was to evaluate the effect of ginseng (Panax ginseng) root extract on the histomorphometric and biochemical characteristics of skin in rainbow trout. Study carried out during 60 days within 4 experiments, each with 3 replicates. In each replication 60pcs of fry with average weight of 15gr. were randomly distributed. The experimental groups were fed with aqueous extract of P. Ginseng at 100, 150 & 200 gr./kg. diet respectively. Results obtained from the histological study of the skin of the dorsal and ventral regions of the body showed that the average thickness of none of the skin layers, i.e. epidermis, dermis and hypodermis in the groups receiving ginseng did not show a significant difference in comparison with the control group (p>0.05). The average total number of mucoid cells and the average number of chromatophores in all studied groups were significantly higher in the skin of the dorsal area compared to the ventral region (p0.05). With the increase in the amount of ginseng, the number of mucoid cells in both the dorsal and ventral areas increased significantly in a linear pattern compared to the control group (p0.05).
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