Tragacanth, a Novel and Cheap Gelling Agent in Carnation and Miniature Rose Tissue Culture Media
محورهای موضوعی : مجله گیاهان زینتیSoheil Karimi 1 , Hassan Salehi 2 , Fateme Ashiri 3
1 - Department of Horticulture, College of Aburaihan, University of Tehran, Pakdasht, Tehran, Iran.
2 - Department of Horticultural Science, College of Agriculture, Shiraz University, Shiraz, Iran.
3 - Department of Horticultural Science, College of Agriculture, Shiraz University, Shiraz, Iran.
کلید واژه: Rosa, Agar substitute, <i>Dianthus</i>, Natural gum, Nodal segment,
چکیده مقاله :
The natural tragacanth gum, the sap of Astragalus gummifer, has been successfully used as a gelling agent in tissue culture media for in vitro shoot development and proliferation of miniature rose and carnation. Shoot development of nodal segments excised from greenhouse grown plants, was investigated on MS medium solidified with either 2.5, 3.0, 3.5 and 4.0% tragacanth or 0.8% agar as control. The media solidified with tragacanth were more transparent than the media solidified with agar. Viscosity of the tragacanth-gelled media was less than the agar-gelled media, and therefore, putting the explants on the media sometimes resulted in submersion of them into the media and some cases of development of hyperhydric shoots were observed. To increase the firmness of the media, the combinations of agar (0.2 and 0.3%) and tragacanth (2 and 3%) were used. The viscosity of the combinatory media was as good as the medium containing only 0.8% agar (control). Moreover, the explants productivity and growth obtained in these combinations were better than those recorded on the control medium. Being a plant product, biodegradable and environmental friendly, and 10 times cheaper than agar, tragacanth gum would definitely be useful for reducing costs, particularly in the plant tissue culture industry.
در این پژوهش صمغ کتیرا، شیره گیاهAstragalus gummifer، به صورت موفقیتآمیزی به عنوان عامل جامدکننده محیطهای کشت بافت برای رشد و پرآوری درون شیشه ای شاخساره رز مینیاتور و میخک مورد استفاده قرار گرفت. رشد شاخساره حاصل از قلمه های تک گره گیاهان پرورش یافته در گلخانه روی محیط MS جامد شده با غلظت های مختلف کتیرا شامل 5/2، 3، 5/3 و 4 درصد یا آگار 8/0 درصد به عنوان شاهد مورد ارزیابی قرار گرفت. محیطهای جامد شده با کتیرا نسبت به محیط کشتهای حاوی آگار شفافیت بیشتری داشتند. استحکام کمتر محیط کشتهای جامد شده با کتیرا نسبت به آگار سبب افتادن برخی از ریزنمونه ها در محیط کشت و شیشه ای شدن تعدادی از آنها را در پی داشت. برای افزایش استحکام محیط، ترکیبی از آگار (2/0 و 3/0 درصد) و کتیرا (2 و 3 درصد) استفاده گردید. استحکام محیط های ترکیبی به خوبی محیط حاوی آگار 8/0 درصد بود. افزون بر این، پرآوری و رشد ریزنمونه ها در این محیط ها بیشتر از محیط شاهد ارزیابی گردید. کتیرا به عنوان یک فرآورده گیاهی، قابل تجزیه توسط میکروارگانیسمها و سازگار با محیط زیست بوده و افزون بر این قیمتی در حد یک دهم آگار دارد، از این رو می تواند جهت کاهش هزینهها، به ویژه در صنعت کشت بافت گیاهی، مورد استفاده قرار گیرد.
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