The Enhancement of Drought Stress Tolerance of Kentucky Bluegrass by Prohexadione-Calcium Treatment
محورهای موضوعی : مجله گیاهان زینتیJavad Rezapour Fard 1 , Mohsen Kafi 2 * , Roohangiz Naderi 3
1 - Department of Horticultural Science, Faculty College of Agricultural Science and Natural Resources,
University of Tehran, Karaj, Iran
2 - Department of Horticultural Science, Faculty College of Agricultural Science and Natural Resources,
University of Tehran, Karaj, Iran
3 - Department of Horticultural Science, Faculty College of Agricultural Science and Natural Resources,
University of Tehran, Karaj, Iran
کلید واژه:
چکیده مقاله :
Kentucky bluegrass (Poa pratensis L.) is one of the most widely used turfgrasses for home lawns, golf courses, parks, and athletic fields in temperate climates. Oxidative stress caused by drought stress is a major limiting factor for grass cultivation in arid and semi-arid regions. The objective of this study was to investigate whether Prohexadione-Calcium (Pro-Ca) may play a role in promoting drought tolerance in Kentucky bluegrass. Pro-Ca was exogenously applied as a foliar spray at the rate of 0, 7.5, 15 or 25 mg a.i./m2 to well established kentucky bluegrass under wellwatered(100 of field capacity) or drought-stressed (70 and 40% of field capacity) conditions. The effect of Pro-Ca on the growth physiology, drought stress response, antioxidant activity, and lipid peroxidation of Kentucky bluegrass exposed to drought stress was measured during 28 days at 7 days interval. Pro-Ca treated kentucky bluegrass exposed to drought stress had higher relative water content (RWC) and turf quality, and lower electrolyte leakage and malondialdehyde (MDA) content as membrane integrity indicators, compared with untreated plants. Pro-Ca application significantly increased the antioxidant enzymes superoxide dismutase (SOD), ascorbate peroxidase (APX) and catalase (CAT) activities under well-watered and drought conditions, but this increase is more evident in drought stressed plants. Pro-Ca application at 15 mg/m2 was found to be more effective in alleviating drought stress damage in kentucky bluegrass. The results from this study suggest that Pro-Ca enhanced drought stress tolerance in kentucky bluegrass by maintaining higher RWC and membrane stability.
کنتاکی بلوگراس (پوآ پراتنسیس) یکی از گونه های پرکاربرد در چمن های خانگی، زمینهای گلف، پارک ها و میادین ورزشی در اقلیم های معتدله می باشد. تنش اکسیداتیو ناشی از خشکی یکی از عوامل اصلی محدود کننده کاشت چمن در مناطق خشک و نیمه خشک می باشد. در این مطالعه، نقش احتمالی پروهگزادیون کلسیم در افزایش تحمل به خشکی در چمن کنتاکی بلوگراس مورد ارزیابی قرار گرفت. به این منظور، پروهگزادیون کلسیم با غلظت های 0، 5/7، 15 و 25 میلی گرم ماده موثره در متر مربع بر روی اندام هوایی چمن کاملا یکدست و استقرار یافته کنتاکی بلوگراس محلول پاشی شده و سپس چمن ها تحت تنش خشکی عدم تنش (100 درصد ظرفیت زراعی) و تنش 70 و 40 درصد ظرفیت زراعی قرار گرفتند. اثر پروهگزادیون کلسیم بر روی فیزیولوژی رشد، پاسخ به تنش خشکی، فعالیت آنتی اکسیدانی و پراکسیداسیون لیپیدی چمن در شرایط خشکی در طول 28 روز و با فواصل زمانی 7 روز مورد ارزیابی و اندازه گیری قرار گرفت. چمن های تیمار شده با پروهگزادیون کلسیم، در شرایط تنش خشکی دارای محتوای نسبی آب بیشتر و کیفیت کلی بالاتری بوده و مقادیر نشت یونی و میزان مالون دی آلدهید به عنوان شاخص های انسجام غشا، در چمن های تیمار شده، در مقایسه با چمن های تیمار نشده پایین تر بود. کاربرد پروهگزادیون کلسیم باعث افزایش فعالیت آنزیم های آنتی اکسیدانی سوپراکسید دیسموتاز، آسکوربات پراکسیداز و کاتالاز در شرایط تنش خشکی و عدم تنش گردید ولی این افزایش در شرایط تنش خشکی مشهودتر بود. غلظت 15 میلی گرم در متر مربع ماده موثره پروهگزادیون کلسیم در کاهش صدمات تنش خشکی در کنتاکی بلوگراس موثرتر بود. نتایج حاصل از این تحقیق نشان داد که پروهگزادیون کلسیم با افزایش محتوای نسبی آب برگ و حفظ پایداری غشا باعث افزایش تحمل به خشکی در چمن کنتاکی بلوگراس می گردد.
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