Effect of BAP and NAA on Micropropagation of Caladium bicolor (Aiton) Vent., an Ornamental Plant
محورهای موضوعی : مجله گیاهان زینتی
Shima Seydi
1
,
Naser Negahdar
2
,
Raziye Taghizadeh Andevari
3
,
Mohammad Hossein Ansari
4
,
Behzad Kaviani
5
1 - Department of Horticultural Science, Rasht Branch, Islamic Azad University, Rasht, Iran
2 - Master of Science and Ph.D. Student, Hyrcan Agricultural Sciences and Biotechnology Research Institute, Amol, Iran
3 - Master of Science, Hyrcan Agricultural Sciences and Biotechnology Research Institute, Amol, Iran
4 - Department of Agronomy, Rasht Branch, Islamic Azad University, Rasht, Iran
5 - Department of Horticultural Science, Rasht Branch, Islamic Azad University, Rasht, Iran
کلید واژه: tissue culture, In vitro propagation, plant growth regulators, Araceae,
چکیده مقاله :
Leaf explants of kaladium dwubarwneCaladium bicolor(Aiton) Vent., were cultured in vitro on MS media supplemented with 25 different concentrations of BAP and NAA in order to determine the appropriate concentrations for micropropagation. All combinations induced callus formation on explants. Callus production on leaves explants grown on control medium was very low. The medium enriched with 4 mg l-1 BAP + 0.5 mg l-1 NAA was the most effective for callus formation. The highest number of shoots (6.43 per explant) and roots (5.56 per explant) were regenerated on media containing 1 mg l-1 BAP + 0.5 mg l-1 NAA and 3 mg l-1 BAP + 0.5 mg l-1 NAA, respectively. The regenerated plantlets were grown in a greenhouse and acclimatized successfully.
ریزنمونه‌های برگ کالادیوم در شرایط درون شیشه‌ای روی محیط‌های MS همراه با 25 غلظت مختلف از BAP و NAA به‌منظور تعیین غلظت‌های مناسب برای ریزازدیادی کشت شدند. تمام ترکیبات تشکیل کالوس را روی ریز نمونه‌ها القا کردند. تولید کالوس روی ریزنمونه‌های برگی رشد یافته روی محیط شاهد بسیار کم بود. محیط غنی شده با 4 میلی‌گرم بر لیتر BAP + 5/0 میلی‌گرم بر لیتر NAA برای تشکیل کالوس موثرترین بودند. بیشترین تعداد نوشاخه‌ها (43/6 در ریزنمونه) و بیشترین تعداد ریشه‌ها (56/5 در ریز نمونه) به‌ترتیب روی محیط‌های حاوی یک میلی‌گرم بر لیتر BAP + 5/0 میلی‌گرم بر لیتر NAA و 3 میلی‌گرم بر لیتر BAP + 5/0 میلی‌گرم بر لیتر NAA تولید شدند. گیاهچه‌های باززایی شده در شرایط گلخانه‌ای رشد داده شدند و بطور موفقیت‌آمیزی سازگار گردیدند.
Ahmad, E.U., Hayashi, T. and Yazawa, S. 2004. Auxins increase the occurrence of leaf-colour
variants in Caladium regenerated from leaf explants. Scientia Horticultureae, 100: 153–159.
Ahmad, E.U., Hayashi, T., Zhu, Y., Hosowaka M. and Yazawa, S. 2002. Lower incidence of variants
in Caladium bicolor Ait. plants propagated by culture of explants from younger tissue. Scientia
Horticultureae, 96: 187–194.
Ali, A., Munawar, A. and Naz, S. 2007. An in vitro study on micropropagation of Caladium bicolor.
International Journal of Agriculture and Biology, 9 (5): 731-735.
Chan, L., Tan, C.M. and Chew, G.S. 2003. Micropropagation of the Araceae ornamental plants.
Acta Horticulture, 606: 383–390.
Chawla, H.S. 2009. Introduction to Plant Biotechnology. Springer.
Chu, Y. and Yazawa, S. 2001. The variation and the hereditary stability on leaf character of plantlets
regenerated from micropropagation in Caladiums. Journal of Chinese Society for Horticultural
Science, 47: 59–67.
Deng, Z. and Harbaugh, B.K. 2006. ’Garden White’-A large white fancy-leaved Caladium for sunny
landscapes and large containers. HortScience, 41: 840–842.
Deng, Z., Hu, J., Goktepe, F. and Harbaugh, B. 2007. Assessment of genetic diversity and relationships
among caladium cultivars and species using molecular markers. Journal of American Society
for Horticultural Science, 132: 147–277.
El-Agamy, S.Z. 2009. In vitro propagation of some grape root stocks. Acta Horticulturae, 839: 25–132.
Fráguas, C.B., Pasqual, M., Dutra, L.F. and Cazzeta, O. 2004. Micropropagation of fig (Ficus carica
L.) ‘Roxo de Valinhos’ plants. In Vitro Cell Development and Biology Plant, 40: 471–474.
George, E.F., Hall, M.A. and Klerk, J.D. 2008. Plant propagation by tissue culture. The Background,
Springer, 1: 65–75.
Gill, R.I.S., Gill, S.S. and Gosal, S.S. 1994. Vegetative propagation of Eucalyptus tereticornis Sm.
through tissue culture. Bangladesh Association for Plant Tissue Culture, 4: 59–67.
Gomes, F. and Canhoto, J.M. 2003. Micropropagation of Eucalyptus nitens Maiden (Shining gum).
In Vitro Cell Development and Biology Plant, 39: 316–321.
Gonçalves, J.C., Diogo, G. and Amancio, S. 1998. In vitro propagation of chestnut (Castanea sativa
× Castanea crenata): effects of rooting treatments on plant survival, peroxidise activity and
anatomical changes during adventitious root formation. Scientia Horticulturae, 72: 265–275.
Jain, S.M. and Ochatt, S.J. 2010. Protocols for in vitro propagation of ornamental plants. Springer
Protocols. Humana Press.
Kaviani, B. 2015. Some useful information about micropropagation. Journal of Ornamental Plants,
5 (1): 29-40.
Kaviani, B., Hashemabadi, D., Khodabakhsh, H., Onsinejad, R., Ansari, M.H. and Haghighat, N.
2015. Micropropagation of Begonia rex Putz. by 6-benzyladenine (BA) and α-naphthalene
acetic acid (NAA). International Journal of Biosciences, 6 (5): 8-15.
Mineo, L. 1990. Plant tissue culture techniques. Pages 151-174, In: Tested studies for laboratory
teaching. Vol. 11. (C.A. Goldman, ed.). Proceeding of the Eleventh Workshop/Conference
of Association Biology and Laboratory Education, (ABLE), 195 p.
Mujib, A., Bandyopadhyay, S. and Ghosh, P.D. 2000. Tissue culture derived plantlet variation in
Caladium bicolor L. An important ornamental. Plant Tissue Culture, 10: 149–55.
Murashige, T. and Skoog, F. 1962. A revised medium for rapid growth and bioassays with tobacco
tissue culture. Physiologia Plant, 15: 473–497.
Nhut, D.T. 2003. The control of In vitro direct main stem formation of Lilium longiflorum derived
from receptacle culture and rapid propagation by using in vitro stem nodes. Plant Growth
Regulation, 40 (2): 179–184.
Nhut, D.T., Hai, N.T. and Phan, M.X. 2010. A highly efficient protocol for micropropagation of
Begonia tuberous. In: Jain SM, Ochatt SJ (eds) Protocols for In Vitro Propagation of Ornamental
Plants, Springer protocols. Humana Press, pp. 15-20.
Pati, P.K., Rath, S.P., Sharma, M., Sood, A. and Ahuja P.S. 2005. In vitro propagation of rose – a
review. Biotechnology Advance, 94–114.
Pierik, R.L.M. 1987. In vitro culture of higher plants. Dordrecht, The Netherlands, Martinus Nijhoff,
45–82.
Siddiqui, F.A., Naz, S. and Iqbal, J. 1993. In vitro propagation of carnation. Advances in plant tissue
culture. Proceedings of the 3rd National Meeting of Plant Tissue Culture, Pakistan, pp. 43–47.
Thepsithar, C., Thongpukdee, A. and Chiensil, P. 2010. Micropropagation of Caladium bicolor (Ait.)
Vent. 'Thep Songsil' and incidence of somaclonal variants. International Society for Horticultural
Science, XXIII International EUCARPIA Symposium, Abstract.
Van Staden, D., Zazimalora, E. and George, E.F. 2008. Plant growth regulators, II: CKs, their analogues
and inhibitors. In: Plant Propagation by Tissue Culture (edn. 3) (George E.F., et al. eds.),
pp. 205–226, Springer.
Zhu, Y., Yazawa, S. and Asahira, T. 1993. Varietal differences in leaf color variation of plants regenerated
from in vitro culture of leaf blade in Caladium cultivars. Journal of Japanese Society for
Horticultural Science, 62: 431–435.