Pre-Harvest Application of γ-Aminobutyric Acid (GABA) and CaCl2 Improves the Vase Life of Rose cut cv. ‘Jumilia’
محورهای موضوعی : مجله گیاهان زینتیNarges Ehsanimehr 1 , Mehdi Hosseinifarahi 2 , Moslem Abdipour 3 , Saeid Eshghi 4
1 - PhD Student, Department of Horticultural Science, Yasuj Branch, Islamic Azad University, Yasuj, Iran
2 - Associate Professor, Department of Horticultural Science, Yasuj Branch, Islamic Azad University, Yasuj, Iran
3 - Assistant Professor, Kohgiluyeh and Boyerahmad Agricultural and Natural Resources Research and Education Center, Agricultural Research, Education and Extension Organization (AREEO), Yasuj, Iran
4 - Department of Horticultural Science, College of Agriculture, Shiraz University, Shiraz, Iran
کلید واژه: Calcium, Polyamines, Chlorophyll content, ACC synthase activity,
چکیده مقاله :
Rose is an important cut flower crop throughout the world in the floriculture industry. One of the most important problems of cut roses is their short post-harvest vase life, which imposes huge losses for cut plant production and trade in the market. This study aimed to investigate the effects of γ-aminobutyric acid (GABA) and calcium chloride (CaCl2) on vase life and the ACC synthase activity of cut rose cv. ‘Jumilia’ flowers. A factorial experiment based on a completely randomized design (CRD) with 12 treatments and 3 replications was carried out in a soilless culture greenhouse. The treatments included GABA in four concentrations (0, 20, 40, and 60 mM) and CaCl2 in three concentrations (0, 0.75, and 1.5% as foliar application). The combined application of GABA and CaCl2 significantly prolonged the vase life of the cut rose flowers compared to the control. The results showed that the highest and lowest vase life were obtained from the flowers treated with 20, 40, or 60 mM GABA and 0.75 % CaCl2 and the untreated flowers, by 19.1, 18.86, 18.74, and 13.85 days, respectively. The pre-harvest application of GABA and CaCl2 inhibited the ACC synthase activity. The highest and lowest ACC synthase activity were observed in plants treated with 40 mM GABA and 1.5 and 0.75% CaCl2 and the untreated flowers by 3.57, 3.96, and 8.18 nmol g-1 FW, respectively. The combined application of GABA and CaCl2 increased the Ca content by 100.95% compared to the control plants. The highest and lowest total chlorophyll contents in the leaves were related to the plants treated with 60 mM GABA and 0.75 and 1.5% CaCl2 and the untreated flowers by 8.4, 8.24, and 6.22 mg g-1 FW, respectively. The foliar application of GABA and CaCl2 significantly (P < 0.01) increased the polyamines (PAs) contents, such as putrescine (Put), spermidine (Spd), and spermine (Spm), in the petals of the treated rose flowers. So, the use of foliar application GABA 60 mM and CaCl2 1.5% is recommended due to the 38% increase in the vase life of cut rose flower.
این مطالعه با هدف بررسی اثرات گاما آمینو بوتیریک اسید (GABA) و کلرید کلسیم (CaCl2) بر عمر گلجایی و فعالیت آنزیم ACC سنتاز گل رز شاخه بریده رقم جومیلیا انجام شد. یک آزمایش به صورت فاکتوریل در قالب طرح کاملاً تصادفی با 12 تیمار و 3 تکرار در گلخانه کشت بدون خاک انجام شد. تیمارها شامل گابا در چهار غلظت (0، 20، 40 و 60 میلی‌مولار) و کلرید کلسیم در سه غلظت (0، 0/75 و 1.5 درصد به صورت محلول‌پاشی) بود. کاربرد ترکیبی گابا و کلرید کلسیم به طور قابل توجهی عمر گلجایی گل رز شاخه بریده را در مقایسه با شاهد افزایش داد. نتایج نشان داد که بیشترین و کمترین عمر گلجایی در گیاهان تیمار شده با گابا 20، 40 یا 60 میلی مولار و 0/75 درصد کلرید کلسیم کلسیم و گل های تیمار نشده به ترتیب با 19/1، 18/86، 18/74 و 13/85 روز به دست آمد. کاربرد قبل از برداشت گابا و کلرید کلسیم فعالیت آنزیم ACC سنتاز را مهار کرد. بیشترین و کمترین فعالیت ACC سنتاز در گیاهان تیمار شده با 40 میلی مولار گابا و کلرید کلسیم 1/5 و 0/75 درصد و گل های تیمار نشده به ترتیب با 3/57، 3/96 و 8/18 نانومول بر گرم وزن تر مشاهده شد. کاربرد ترکیبی گابا و کلرید کلسیم میزان کلسیم را 100/95 درصد نسبت به گیاهان شاهد افزایش داد. بیشترین و کمترین میزان کلروفیل کل در برگها مربوط به گیاهان تیمار شده با 60 میلی مولار گابا و 0/75 و 1/5 درصد کلرید کلسیم و گل های تیمار نشده به ترتیب با 8/4، 8/24 و 6/22 میلی گرم بر گرم وزن تر بود. محلول پاشی گابا و کلرید کلسیم به طور قابل توجهی (P < 0.01) باعث افزایش محتویات پلی آمین ها مانند پوترسین (Put)، اسپرمیدین (Spd) و اسپرمین (Spm) در گلبرگ های گل رز تیمار شده شد. بنابراین استفاده از محلول‌پاشی با 60 میلی مولار GABA و کلسیم کلرید 1/5 درصد به دلیل افزایش 38 درصدی عمر گل رز بریده توصیه می‌شود.
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