Evaluation of Cytotoxic and Anti-microbial Activities of the Methanolic Leaf Extracts of Sansevieria zeylanica (L.) Willd. Against the Human Breast Cancer, MDA-MB-231 Cell Lines
محورهای موضوعی : مجله گیاهان زینتی
Somashekara Rajashekara
1
(Centre for Applied Genetics, Department of Studies in Zoology, Bangalore University, Jnana Bharathi Campus, Off Mysuru Road, Bengaluru 560 056, India)
Nuguri Chudamani
2
(Centre for Applied Genetics, Department of Studies in Zoology, Bangalore University, Jnana Bharathi Campus, Off Mysuru Road, Bengaluru 560 056, India)
Priya Rautela
3
(Centre for Applied Genetics, Department of Studies in Zoology, Bangalore University, Jnana Bharathi Campus, Off Mysuru Road, Bengaluru 560 056, India)
Sharvani Hegde
4
(Centre for Applied Genetics, Department of Studies in Zoology, Bangalore University, Jnana Bharathi Campus, Off Mysuru Road, Bengaluru 560 056, India)
ShivaReddy Swaroopa
5
(Centre for Applied Genetics, Department of Studies in Zoology, Bangalore University, Jnana Bharathi Campus, Off Mysuru Road, Bengaluru 560 056, India)
کلید واژه: Cytotoxic effect, Anti-microbial activity, MDA-MB-231 cell lines, Methanolic leaf extract,
چکیده مقاله :
Sansevieria zeylanica is a species of xerophytic perennial herbs with a wide range of distribution found in dry tropical and subtropical parts of the world. It is a wonderful medicinal plant and holds the widespread range of beneficial properties. The aim of the study is to determine the cytotoxic activities of the human breast cancer - MDA-MB-231 cell lines and to evaluate the anti-microbial activities by employing the methanolic leaf extracts of S. zeylanica plants. The main experiment was to determine the susceptibility of a microbial organisms against the methanolic leaf extracts of S. zeylanica. The percentage growth inhibition was calculated and concentration of sample to inhibit cell growth by 50 % (IC50) values was generated for the percentage viability. The leaf extracts produced from S. zeylanica plants showed a cytotoxic effect against the human breast cancer - MDA-MB-231 cell lines. The IC50 value of 1167.78 µg/ ml was obtained for the MDA-MB-231 cell lines after the exposure of 24 h treatment. The increase in cell death of MDA-MB-231 cell lines with an increase in the concentration indicates that the methanolic leaf extract of S. zeylanica plants was found active. For the first time, we made an attempt to examine the biological properties of methanolic leaf extracts from S. zeylanica plants with reference to their wide applications. The results obtained support for the therapeutic applications and this acts as an anti-microbial agent. These studies showed the results from the phytochemical screening, anti-microbial and cytotoxic activities of S. zeylanica indicating that this plant as a “natural herbal source”. This can be used for its pharmaceutical and pharmacological applications in a large-scale industry.
گیاه Sansevieria zeylanica یک گونه علفی چند ساله مناطق خشک است که در قسمتهای گرمسیر و نیمه گرمسیر دنیا پراکندگی دارد. این گیاه، یک گیاه شگفتانگیز دارویی است و مزایای زیادی دارد. هدف این مطالعه تعیین فعالیتهای سمیت سلولی در سرطان سینه در انسان روی لاینهای سلولی MDA-MB-231 و نیز ارزیابی خاصیت ضد میکروبی با استفاده از عصاره برگ متانولی گیاه S. zeylanica است. آزمایش اصلی تعیین حساسیت میکروبها در برابر عصارههای متانولی برگ گیاه S. zeylanica است. درصد بازدارندگی رشد اندازهگیری شد و غلظت نمونه برای ممانعت از رشد سلول تا 50 درصد (IC50) برای درصد زندهمانی تعیین شد. عصارههای برگی بدست آمده از گیاه S. zeylanica روی لاینهای سلولیMDA-MB-231 سرطان سینه در انسان اثر سمیت سلولی نشان دادند. شاخص IC50 (78/1167 میکروگرم در میلیلیتر) پس از تیمار 24 ساعته روی این لاینها بدست آمد. افزایش مرگ سلولی روی لاینهای MDA-MB-231 با افزایش غلظت عصاره نشان میدهد که عصاره متانولی برگ این گیاه فعال است. برای اولین بار، ما تلاش کردیم تا خصوصیات بیولوژیکی عصاره برگ متانولی گیاه S. zeylanica را ارزیابی کنیم. نتایج حاصل کاربرد درمانی را تایید کرد و میتواند برای کاربرد وسیع بهعنوان مادهای ضد میکروب، مدرک معتبری باشد. در این مطالعات نتایج غربالگری فعالیتهای فایتوشیمیایی، ضد میکروبی و سمیت گیاه S. zeylanica تایید کرد که این گیاه میتواند بهعنوان منبع گیاهی طبیعی مورد استفاده قرار گیرد. این گیاه میتواند برای مصارف دارویی و داروشناسی در مقیاس وسیع و بصورت صنعتی استفاده شود.
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