Physiological Changes During Cold Storage of Lilium ledebourii (Baker) Boiss. Bulb, a Rare Indigenous Species to Iran
محورهای موضوعی : مجله گیاهان زینتیFatemeh Abazarifard 1 , Seyed Najmmaddin Mortazavi 2 , Rohangiz Naderi 3 , Sepideh Kalateh Jari 4
1 - Department of Horticultural Sciences, Science and Research Branch, Islamic Azad University, Tehran, Iran
2 - Department of Horticultural Sciences, Faculty of Agriculture, University of Zanjan, Zanjan, Iran
3 - Department of Horticultural Sciences, Faculty of Agricultural Science and Engineering, University of Tehran, Tehran, Iran
4 - Department of Horticultural Sciences, Science and Research Branch, Islamic Azad University, Tehran, Iran
کلید واژه: Hormone, Saponin, Lily, Soluble sugars, Cold period,
چکیده مقاله :
Lilium ledebourii is a native and endangered rare species in Iran. It requires a cold period for proper growth and flowering. This experiment was performed to investigate the effects of cold storage on changes of endogenous abscisic acid (ABA), indole-3-acetic acid (IAA), gibberellic acid (GA3), sucrose, fructose, glucose, and total saponin during dormancy of Lilium ledebourii bulbs. The bulbs of lily were stored at 1 or 7 ºC, for 0, 30, 60, 90, and 120 days, and after each period removed from storage and analyzed using HPLC system. The results indicated that, cold storage at 1 ºC for 120 days decreased the levels of ABA while, 7 ºC in the same duration increased its content, slightly. The levels of IAA increased at 1 ºC storage, while remained almost constant at 7 ºC. Cold treatment at 1 ºC increased the levels of GA3, while storage at 7 ºC caused slight decrease in its content. Sucrose and fructose concentrations increased in both temperatures during the storage, while minor reduction in glucose content was observed. Storage at 7 ºC for 120 days induced the highest content of total saponin. The magnitude of changes in hormones and soluble sugars measured at 1 ºC was greater than that at 7 ºC.
سوسن چلچراغ یکی از گونههای نادر بومی و در معرض خطر انقراض است که برای رشد و گلدهی مناسب نیاز به یک دوره سرمایی دارد. این آزمایش بهمنظور بررسی اثرات سرمادهی پیاز بر تغییرات تنظیم کنندههای رشد درونزای آبسیزیک اسید (ABA)، ایندول استیک اسید (IAA)، جیبرلیک اسید (GA3) و قندهای محلول ساکارز، فروکتوز، گلوکز و همچنین ساپونین تام در طول خواب پیاز سوسن چلچراغ انجام شد. بدینمنظور پیازهای این گیاه در دمای 1 یا 7 درجه سانتیگراد به مدت 0، 30، 90،60 و 120 روز قرار گرفتند و پس از پایان هر دوره، از انبار برداشت و توسط دستگاه HPLC تجزیه و تحلیل شدند. نتایج نشان داد که سرمادهی پیازها در یک درجه سانتیگراد به مدت 120 روز باعث کاهش سطح ABA شد در حالیکه 7 درجه سانتیگراد در همان مدت زمان سبب افزایش جزیی در سطح این هورمون شد. تیمارهای سرمایی یک درجه سانتیگراد سبب افزایش سطح IAA و GA3 شدند اما در 7 درجه سانتیگراد سطح IAA تقریبا ثابت باقی ماند و GA3 کاهش جزئی نشان داد. غلظتهای ساکارز و فروکتوز در هر دو تیمار دمایی افزایش یافتند در حالیکه مقدار گلوکز کاهش یافت. انبار پیازها در 7 درجه سانتیگراد به مدت 120 روز بالاترین سطح ساپونین تام را سبب شد. تغییرات هورمونها و قندهای اندازه گیری شده در 1 درجه سانتیگراد بیشتر از 7 درجه سانتیگراد بود.
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