Effect of Foliar Application of Proline on Morphological and Physiological Traits of Calendula officinalis L. under Drought Stress
محورهای موضوعی : مجله گیاهان زینتی
Sophia Soroori
1
,
Elham Danaee
2
,
Khodayar Hemmati
3
,
Alireza Ladan Moghadam
4
1 - Ph.D. Student, Medicinal Plant, Department of Horticultural Sciences, Aliabad Katoul Branch, Islamic Azad University, Aliabad Katoul, Iran
2 - Assistant Professor, Department of Horticultural Sciences, Garmsar Branch, Islamic Azad University, Garmsar, Iran
3 - Associate Professor, Department of Horticulture Sciences, Gorgan University of Agricultural Sciences and Natural Resources, Gorgan, Iran
4 - Associate Professor, Department of Horticultural Sciences, Garmsar Branch, Islamic Azad University, Garmsar, Iran
کلید واژه: Chlorophyll, Carotenoid, Field capacity, antioxidant activity, Plant longevity,
چکیده مقاله :
Drought stress is one of the most important abiotic stresses that seriously reduces the production of ornamental plants such as pot marigold. Proline is one of the active amino acids in the osmotic regulation phenomenon and it has an effective role in reducing cell damage and improving drought tolerance. A pot experiment as factorial arrangement in a completely randomized design with three replications was performed in order to study the effect of foliar application of proline (0, 50 and 100mg L-1) on some morpho- physiological characteristics of pot marigold under drought stress (normal irrigation, 25, 50 and 75 % field capacity). The results showed that the highest fresh and dry weight of aerial organ, longevity of the flower on the plant, carotenoid and total chlorophyll were observed in normal irrigation+ proline 100 mg L-1. The highest fresh and dry root weight, root length and total antioxidant activity (DPPH) were for 75 % FC + proline 100 mg L-1. The highest plant height and number of flowers were in normal irrigation+ proline 50 mg L-1. The highest proline content was belonged to 25 % FC+ proline 100 mg L-1. In general, the results of this experiment showed that application of proline can partially compensate for the adverse effects of dehydration and on the other hand, despite the fact that the pot marigold plant is always able to survive in drought stress, it is recommended to irrigate 75% FC in order to increase pot marigold performance.
تنش خشکی یکی از مهم‌ترین تنش‌های غیرزنده است که بهطور جدی تولید گیاهان زینتی از جمله همیشه بهار را کاهش می‌دهد، پرولین یکی از اسید آمینه‌های فعال در پدیده تنظیم اسمزی است، نقش موثری در کاهش آسیب‌های سلولی و بهبود تحمل به تنش خشکی دارد. آزمایش بصورت فاکتوریل در قالب طرح آماری کاملاً تصادفی با سه تکرار بهمنظور بررسی تاثیر محلولپاشی پرولین (صفر، 50 و 100 میلی‌گرم در لیتر) بر خصوصیات مورفولوژیک و فیزیولوژیک گیاه همیشه بهار(Calendula officinalis L.) تحت تنش خشکی (بدون تنش(شاهد)، 25، 50 و 75 درصد ظرفیت زراعی) انجام شد. نتایج حاصل از این آزمایش نشان داد، بیشترین وزن تر و خشک اندام هوایی و ماندگاری گل روی بوته، کاروتنوئید و کلروفیل کل در تیمار پرولین 100 میلی‌گرم در لیتر و ظرفیت زراعی شاهد بود، بیشترین وزن تر و خشک ریشه، طول ریشه و فعالیت آنتی‌اکسیدان کل (DPPH) در تیمار پرولین 100 میلی‌گرم در لیتر و ظرفیت زراعی 75 درصد، بیشترین ارتفاع گیاه و تعداد گل در تیمار پرولین 50 میلی‌گرم در لیتر و ظرفیت زراعی شاهد، بیشترین پرولین در تیمار پرولین 100 میلی‌گرم در لیتر و ظرفیت زراعی 25 درصد بود. بهطور کلی نتایج این آزمایش نشان داد، محلولپاشی پرولین می‌تواند اثرات نامطلوب کم آبی را تا حدودی جبران کند و از طرفی با وجود اینکه گیاه همیشه‌بهار توانایی ادامه حیات را در شرایط تنش خشکی دارا است، اما در جهت بهبود افزایش عملکرد همیشه بهار آبیاری در سطح 75 درصد ظرفیت زراعی توصیه می‌شود.
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