Morphological and Biochemical Responses of Gerbera (Gerbera Jamesonii L.) to Application of Silica Nanoparticles and Calcium Chelate under Hydroponic State
محورهای موضوعی : مجله گیاهان زینتی
Tahereh Tofighi Alikhani
1
(
Department of Horticultural Sciences, Science and Research Branch, Islamic Azad University, Tehran, Iran
)
Seyed Jalal Tabatabaei
2
(
Shahed University, Department of Horticultural Sciences and Medicinal Plant Research Center, Tehran, Iran
)
Ali Mohammadi Torkashvand
3
(
Department of Soil Science, Science and Research Branch, Islamic Azad University, Tehran, Iran
)
Ahmad Khalighi
4
(
Department of Horticultural Sciences, Science and Research Branch, Islamic Azad University, Tehran, Iran
)
Daryush Talei
5
(
Medicinal Plants Research Center, Shahed University, Tehran, Iran
)
کلید واژه: Chlorophyll index, Ionic leakage, Phenylalanine ammonia-lyase enzyme, Stem bending,
چکیده مقاله :
The purpose of this study was to investigate the response of physicochemical properties of gerbera (Gerbera Jamesonii L.) to silica nanoparticles (Nanoparticle-SiO2) and calcium chelate (Ca-Chelate) in nutrient solutions. A factorial experiment based on completely randomized design with two factors was conducted in four replicates. The first factor the concentration of Nanoparticle-SiO2 in nutrient solutions with four levels (0, 20, 40, 80 mg L-1), and the second factor was the concentration of Ca-Chelate in the nutrient solution with four levels (0, 60, 120 and 240 mg L-1). Flower diameter, peduncle length, leaf area, fresh and dry weights of root, leaf and flower, stem bending, leaf ionic leakage, chlorophyllindex, maximum efficiency of Photosystem II (Fv/Fm), percentages of cellulose, hemicellulose, and holocellulose in stem, and phenylalanine ammonia-lyase activity in leaves were measured. According to the results, in comparison to the control, application of 240 mg L-1 Ca-Chelate together with 80 mg L-1 Nanoparticle-SiO2, resulted in 1.5 times less stem bending and decreased leaf ionic leakage by 28%. The highest activity of phenylalanine ammonia-lyase in leaves was observed in the treatment with 60 mg L-1 Ca-Chelate (2.91 unit mg fresh leaves-1). The best treatment in terms of the chlorophyllindex, leaf area, percentages of cellulose and the maximum efficiency of Photosystem II (Fv/Fm) was the treatment with 120 mg L-1 Ca-Chelate and 80 mg L-1 Nanoparticle-SiO2. Therefore, application of 40 mg L-1 Nanoparticle-SiO2 and 60 mg L-1 Ca-Chelate in the hydroponic nutrition solution can be suggested for gerbera plants.
هدف از این مطالعه، بررسی واکنش های فیزیولوژیکی گل ژربرا (Gerbera jamesonii L.)تحت تیمار نانو ذرات سیلیس و کلات کلسیم در محلول غذایی بود. برای این منظور، آزمایشی بهصورت فاکتوریل در قالب طرح کاملا تصادفی با دو فاکتور و چهار تکرار انجام شد. فاکتور اول ذرات نانو سیلس (SiO2-Nano) با چهار سطح صفر، ۲۰، 40 و 80 mg/L) از منبعSiO2 و فاکتور دوم کلات کلسیم (Ca-Ch) با چهار سطح صفر، ۶۰، 120 و 240 mg/L) از منبع Ca-EDTA به صورت کاربرد در محلول غذائی بود. قطر گل، طول دمگل، سطح برگ، وزن تر و خشک ریشه، برگ و گل، خمیدگی ساقه، نشت یونی برگ، شاخص کلروفیل، حداکثر کارایی فتوسیستم II (FV/Fm)، درصد سلولز، همی سلولز و هولو سلولز ساقه و فعالیت آنزیم فنیل آلانین آمونیالیاز برگ اندازهگیری شد. نتایج نشان داد اثر متقابل 240 mg/L Ca-Ch همراه با 80 mg/L SiO2-Nano موجب کاهش 5/1 برابری خمیدگی ساقه و کاهش ۲۸ ٪ نشت یونی برگ نسبت به شاهد شدند. تیمار ترکیبی SiO2-Nano 20 mg/L همراه با 60 mg/L Ca-Ch، سطح برگ را نسبت به شاهد ۵۹ ٪ افزایش دادند. بیشترین میزان فعالیت آنزیم فنیل آلانین آمونیالیاز در برگ در60 mg/L Ca-Ch بود (2.91 unit/mg fresh leaves). مناسبترین غلظت بر شاخص کلروفیل، سطح برگ، درصد سلولز و حداکثر کارایی فتوسیستم II (FV/Fm)، تیمار ترکیبی mg/L Ca-Ch 120 همراه با80 mg/L SiO2-Nano بود. بنابراین، کاربرد تیمار ترکیبی 60 mg/LCa-Ch همراه با 40 mg/L SiO2-Nano در محلول غذائی کشت هیدروپونیک گل ژربرا قابل توصیه میباشد.
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