An Effective Nutrient Media for Asymbiotic Seed Germination and In Vitro Seedling Development of Phalaenopsis ‘Bahia Blanca’

محورهای موضوعی : مجله گیاهان زینتی

Seyedeh Mehrnaz Abbaszadeh ¹ , Seied Mehdi Miri ² , Rouhangiz Naderi ³

1 - Department of Horticulture, Karaj Branch, Islamic Azad University, Karaj, Iran
2 - Department of Horticulture, Karaj Branch, Islamic Azad University, Karaj, Iran
3 - Department of Horticulture, University of Tehran, Karaj, Iran

تاریخ دریافت : 1396/11/20 تاریخ پذیرش : 1397/04/14 تاریخ انتشار : 1397/05/10

کلید واژه: Media, Protocorm-like bodies, Coconut water, Orchid, Peptone,

چکیده مقاله :

Our study was to develop a rapid and successful method for promoting asymbiotic seed germination and growth and development of protocorm like bodies (PLBs) and subsequent plantlets of Phalaenopsis orchid. Four basal media i.e. Murashige and Skoog (MS), half strength MS (½MS), Knudson C (KC) and, Vacin and Went (VW) supplemented with coconut water and peptone were examined for their effectiveness in large-scale multiplication by asymbiotic seed germination. Seed germination, leaf number, root number, root length, seedling length and fresh and dry weight were scored. Well-rooted plantlets 4-cm in height were transferred to a greenhouse in trays containing expanded clay, charcoal, cocopeat and perlite (4:2:1:1) or sphagnum moss. The seed germination frequency and seedling development was found significantly higher in organic growth supplement-enriched MS and ½MS media. The results indicated that the highest seed germination (94 %) was obtained in MS medium supplemented with 100 mg/L coconut water or 1 g/L peptone and ½MS containing 1 or 2 g/L peptone. However, the longest seedling was found in MS or ½MS media supplemented with 100 mg/L coconut water or MS containing 1 g/L peptone. Ninety-seven percent of plantlets survived after 1 month in mixture media expanded clay: charcoal: cocopeat: perlit.

چکیده انگلیسی:

این مطالعه به منظور ایجاد یک روش سریع و موفق برای بهبود جوانه زنی غیرهمزیست بذر و رشد و نمو پروتوکورم های شبه بدنی (PLBs) و گیاهچه های ارکیده فالانوپسیس (Phalaenopsis) انجام شد و تاثیر چهار محیط کشت پایه موراشیگ و اسکوگ (MS)، ½MS، نادسون سی (KC) و واسین و ونت (VW) حاوی شیر نارگیل و پپتون برای تکثیر در مقیاس زیاد بواسطه جوانه زنی غیرهمزیست بذر مورد بررسی قرار گرفت. جوانه زنی بذر، تعداد برگ، تعداد ریشه، طول ریشه، طول گیاهچه و وزن تر و خشک اندازه گیری شد. گیاهچه های خوب ریشه دار شده 4 سانتیمتری در سینی های حاوی رس : ذغال : کوکوپیت : پرلیت (4: 2: 1: 1) یا خزه اسفاگنوم کشت و به گلخانه منتقل شدند. فراوانی جوانه زنی بذر و رشد گیاهچه ها در محیط کشت های MS و ½MS حاوی ترکیبات آلی به طور معنی داری بیشتر بود. نتایج نشان داد که بیشترین درصد جوانه زنی بذر در محیط کشت MS حاوی 100 میلی گرم در لیتر شیر نارگیل یا 1 گرم در لیتر پپتون و ½MS حاوی 1 یا 2 گرم در لیتر پپتون به دست آمد. با این حال، طویل ترین دانهال در MS و یا ½MS همراه با 100 میلی گرم در لیتر شیر نارگیل و یا MS حاوی 1 گرم در لیتر پپتون مشاهده شد. 97 درصد از گیاهچه ها پس از 1 ماه در بستر کشت رس : ذغال : کوکوپیت : پرلیت زنده ماندند.

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