ارزیابی فراوانی کمپیلوباکترها و آرکوباکترها در آب دریای خزر با استفاده از روش های کشت و PCR
محورهای موضوعی : میکروب شناسی پزشکیفهیمه قربانی معین 1 , مسعود قانع 2
1 - دانشگاه آزاد اسلامی، واحد تنکابن، عضو باشگاه پژوهشگران جوان
2 - دانشگاه آزاد اسلامی، واحد تنکابن، گروه میکروبیولوژی
کلید واژه: دریای خزر, کمپیلوباکتر, آرکوباکتر,
چکیده مقاله :
مقدمه و هدف: کمپیلوباکترها یکی از مهمترین عوامل ایجاد کننده عفونت های گوارشی در دنیا معرفی می شوند. آب و مواد غذایی آلوده اصلی ترین راه ورود این باکتری به انسان می باشد. هدف از این پژوهش، جداسازی، شناسایی و تشخیص گونه های مختلف کمپیلوباکتر و آرکوباکتر از آب دریای خزر در شمال ایران بود. مواد و روش ها: تعداد 263 نمونه آب در 4 فصل سال جمع آوری گردید. گونه های کمپیلوباکتر و آرکوباکتر با استفاده از روش استاندارد کشت جداسازی شد و سپس به وسیله ی تست های فنوتایپینگ شناسایی شدند. تائید فنوتایپینگ سویه ها بوسیله روش PCR صورت گرفت. یافته ها: بعد از انجام روش های فنوتایپینگ و استفاده از تکنیک های مولکولی 7 سویه از کمپیلوباکتر ججونی و 14 سویه از آرکوباکتر بوتزلری شناسایی شدند. با توجه به نتایج حاصل، شیوع کمپیلوباکتر ججونی و آرکوباکتر بوتزلری در آب های ساحلی جنوب دریای خزر به ترتیب به میزان 66/2 درصد و 32/5 درصد ارزیابی گردید. نتیجه گیری: نتایج حاصل از این تحقیق به عنوان اولین گزارش جداسازی کمپیلوباکتر ججونی و آرکوباکتر بوتزلری از دریای خزر می باشد. مطلع بودن از چگونگی ورود و گستره زمانی انتشار و بقای کمپیلوباکترها در محیط های آبی می تواند در کنترل کیفیت آب و جلوگیری از بیماری اهمیت داشته باشد.
Background and objective: Campylobacter spp. are important causative agent of gastric infection worldwide, and contaminated water and foods are the major transmission factors of this bacterium to human. The major purpose of this study was isolation, identification and characterization of Campylobacter spp. and Arcobacter spp. from the samples obtained from Caspian Sea in the North of Iran. Materials and methods: 263 water samples were collected throughout four seasons. Campylobacter spp. and Arcobacter spp. were isolated using standard methods and were identified by Phenotyping tests. Finally, the identification of these strains was verified by PCR method. Result: Foloowing phenotyping tests and their confirmation with molecular technique, totally seven Campylobacter jejuni strains and 14 Arcobacter butzelri strains were identified. Based on the results, the prevalence of this bacterium in the coastal waters of the Caspian Sea were evaluated as 2.66 and 5.32 percent. Conclusion: It is the first time that Campylobacter jejuni and Arcobacter butzelri were isolated from Caspian Sea. The epidemiologic studies regarding to the ways of their entrance in an environment and their maintenance in the habitat assist activists to control the water qualification and prevention from distribution of infections.
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