Simulation of cell culture by a packed bed Bioreactor
Subject Areas : Smart & Advanced Materials
1 - دانشگاه علم و صنعت ایران
Keywords: scaffolding, Cell Culture, bioreactor, porous environment,
Abstract :
Background To monitor the data and parameters such as shear stress, hydrostatic pressure, temperature, humidity, and so on in a culture medium, numerical simulation can play an important role and help to design an optimum bioreactor. Materials and methods In the study, in the first step, the simulation was done by a homogenous porosity bed. Then for detection of the effectiveness on the temperature and humidity profile of the solid substrate, a heterogeneous porosity distribution was studied. Results The heterogeneous porosity could cause a variety of permeability and heterogeneous distribution of humidity. The normal distribution of cells was 0.6464. Finally, for uniform distribution of temperature in a designed bioreactor defined and simulated. Conclusion This heterogeneous porosity distribution led to a variable permeability distribution and also led to a heterogeneous humidity distribution in the system. The predicted temperature profile for the distribution of heterogeneous porosity was significantly different from that predicted one for the homogeneous bed. In a designed bioreactor, heterogeneity in each altitude not only could start heating but also reach the thermal equilibrium. This could also occur at the relevant altitude in a homogeneous bed.