مقایسه عملکرد آنتی باکتریال عصاره‌های آبی گیاهان هل، گزنه، سنبل الطیب و زعفران بر روی دو باکتری گرم منفی و گرم مثبت
محورهای موضوعی : میکروبیولوژیسپهر مصفاییان 1 , شیلا صفاییان 2 * , zahra Emam-Djomeh 3
1 - دانشجو
2 - گروه بیولوژی دریا، دانشکده علوم و فنون دریایی، دانشگاه آزاد اسلامی واحد تهران شمال
3 - Department of Food Science and Engineering, Faculty of Biosystem Engineering, College of Agriculture, University of Tehran, Karaj, Iran
کلید واژه: عملکرد آنتی باکتریال, هل, گزنه, سنبل الطیب, زعفران,
چکیده مقاله :
چکیده سابقه و هدف :هدف از انجام اين تحقيق بررسي و مقایسه فعاليت آنتی باکتریال عصاره‌هاي هل، گزنه، سنبل الطیب و زعفران بر روي باكتري‌هاي گرم مثبت استافيلوكوكوس اورئوس و گرم منفی اشريشياكلي مي‌باشد. مواد و روش¬ها: پس از تهیه ادویه جات ابتدا عصاره آبی آنها آماده شده و بر اساس وزن خشك هر عصاره غلظت‌هاي مختلف تهيه گردید و با استفاده از روش‌هاي انتشار در سطح آگار و ايجاد چاهك در محيط حداقل غلظت بازدارندگي آنها و با روش رقیق‌سازی در میکرو پلیت حداقل غلظت كشندگي آنها مورد بررسي قرار گرفت. همچنین اثر بازدارندگی و کشندگی مخلوط عصاره‌های آبی نیز تعیین گردید. يافته¬ها: بر این اساس عصاره زعفران دارای بیشترین اثر آنتی باکتریال و عصاره هل دارای کمترین اثر آنتی باکتریال برروی هر دو باکتری گرم مثبت و گرم منفی بودند، گرچه اثر آنتی باکتریال همه عصاره‌ها بر روی باکتری گرم مثبت بیشتر بود. بحث: گیاهان دارویی می توانند بعنوان مکمل دارویی اثرات ضد میکروبی داشته باشند وعوارض کمتری را نسبت به مصرف داروهای شیمیایی داشته باشند.
Abstract Aim and Background: The aim of this study was to evaluate and compare the antibacterial activity of Elettaria cardamomum, Urtica dioica L, Valeriana officinalis and Crocus sativus extracts on Staphylococcus aureus and Escherichia coli. Material and Methods: After preparing the spices first aqueous extracts are prepared based on the dry weight of the extract in different concentrations were prepared by means of diffusion in agar and development of wells in a minimum inhibitory concentration of the dilution Micro MBC plates was studied. Results: Also, the inhibitory effect of MBC and MIC of Aqueous mixture were also determined. Accordingly Crocus sativus extract has the highest antibacterial effect and Elettaria cardamomum extract has the least effect on both Gram-positive and Gram-negative antibacterial, though antibacterial effect on gram-positive bacteria were all extracts. Discussion: Medicinal plants can have antimicrobial effects as a medicinal supplement and have less side effects than using them. Discussion: Medicinal plants can have antimicrobial effects as a medicinal supplement and have less side effects than using chemical drugs.
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