Mechanisms of non-enzymatic antioxidant defense system of different organs of Catharanthus roseus for protective of cell membrane

Subject Areas : Journal of Medicinal Herbs, "J. Med Herb" (Formerly known as Journal of Herbal Drugs or J. Herb Drug)

شاهین  مردانی نژاد ¹ (گروه زیست شناسی، واحد علوم و تحقیقات، دانشگاه آزاد اسلامی، تهران، ایران)
رمضانعلی  خاوری نژاد ² (گروه زیست شناسی، واحد علوم و تحقیقات، دانشگاه آزاد اسلامی، تهران، ایران)
سارا  سعادتمند ³ (گروه زیست شناسی، واحد علوم و تحقیقات، دانشگاه آزاد اسلامی، تهران، ایران)
فرزانه  نجفی ⁴ (دانشکده علوم زیست شناسی، دانشگاه خوارزمی، تهران، ایران)
پرویز  آبرومند آذر ⁵ (گروه شیمی، واحد علوم و تحقیقات، دانشگاه آزاد اسلامی، تهران، ایران)

Received: 2014-05-05 Accepted : 2015-08-04 Published : 2015-01-01

Keywords: Lipid peroxidation, Malondialdehyde, Non-enzymatic antioxidant defense, atharanthus roseus,

Abstract :

Background & Aim: When the level of free radicals is increased and also when both the enzymatic systems and low molecular antioxidants are not sufficient to protect the organism, it seems necessary to get antioxidants from external sources. This study aimed to evaluate the antioxidant potential of different parts of Catharantus roseus Experimental: The antioxidant potential of ethanol extracts of roots, stems, leaves, flowers, seed pods and seeds of C. roseus plant were measured based on 1,1-diphenyl-2-picrylhydrazyl (DPPH) free radical scavenging, inhibition of linoleic acid peroxidation by ferric thiocyanate (FTC), and malondialdehyde inhibition by thiobarbituric acid method (TBA) against the standards: Butylated hydroxytoluene (BHT), Butylated hydroxyanisole (BHA) and ascorbic acid. Results & Discussion: The phenolic content in the root (61.61 ± 2.58 µg of gallic acid per mg of dried weight extract) according to Folin–Ciocalteu method were more than any other organs. In scavenging a half of DPPH• free radicals (IC50), the extract of root, seed, and leaf (238.9 ± 2.02, 253.78 ± 1.42, 277.95 ± 2.56 µg mL-1),respectively, had the best performance. In the inhibition of linoleic acid peroxidation, the root extract had the best inhibitory power after ascorbic acid and BHT, and the leaf and seed extract had the performance similar to BHA. The root (78.68 ± 0.54%), and seed (77.44 ± 0.66%) had the best performance in the inhibition of MDA compared with other extracts. Pearson correlation coefficients between the phenolic content and antioxidant capacity of extracts were high and equal to -0.838, 0.895, and 0.740, respectively, according to DPPH, FTC and TBA methods. Industrial and practical recommendations: The results of this study can be promising in the potential of other applications of the plant organ especially in the inhibition of free radicals and lipids' peroxidation.

References:

Ahmadi, F., Kadivar, M. and Shahedi, M .2007. Antioxidant activity of Kelussia odoratissima Mozaff. in model and food systems. Food Chemistry, 105: 57-64.

Aslam, J., Khan S.H., Siddiqui, Z.H., Fatima, Z., Maqsood, M., Bhat, M.B., Nasim, S.A., Ilah, A., Ahmad, I.Z., Khan, S.A., Mujib, A. and Sharma, M.P. 2010. Catharanthus roseus (L.) G. Don. an important drug: It’s applications and production.  Pharmacie Globale (IJCP), 4: 1–16.

Brand-Williams, W., Cuvelier, M. and Bersel, C. 1995. Use of a free radical method to evaluate antioxidant activity. Food Science and Technology, 28: 25-30.

 Dai, J. and Mumper, R.J. 2010. Plant Phenolics: Extraction, Analysis and Their Antioxidant and Anticancer Properties.  Molecules, 15: 7313-7352.

Elmastas, M., Isildak, O., Turkekul, I. and Temur, N. 2007.  Determination of antioxidant activity and antioxidant compounds in wild edible mushrooms. Journal of Food Composition and Analysis, 20: 337-345.

Ferreira, I.C.F.R., Baptista, P., Vilas-Boas M. and Barros, L. 2007. Free-radical cavenging capacity and reducing power of wild edible mushrooms from northeast Portugal: Individual cap and stipe activity. Food Chemistry, 100:1511–1516.

Ferreres, F., Pereira, D.M., Valentao, P., Andrade, P.B., Seabra, R.M. and Sottomayor, M. 2008. New phenolic compounds and antioxidant potential of Chataranthus roseus. Journal of Agricultural and Food Chemistry, 56: 9967-9974‎.

Grotto, D., Valentin, J., Paniz, C., Schmit, G., Garcia, S.C., Pomblum, V.J. and Farina, M. 2009.  Importance of lipid peroxidation biomarkers and methodological aspects for malondialdehyde quantifcation. Quim Nova, 32:169–174.

Jain, A., Soni, M., Deb, L., Jain, A., Rout, S., Gupta, V. and Krishna, K. 2008. Antioxidant  and   hepatoprotective  activity  of  ethanol  and  aqueous extracts  of  Momordica  dioica  Roxb. leaves.  Journal of Ethnopharmacology, 115: 61-66. 

Kikuzaki, H. and Nakatani, N. 1993. Antioxidant effects of some ginger constituents. Journal of Food Science, 58: 1407-1410.

Msagati, T.A.M. 2013. Chemistry of food additives and preservatives. vol. 1. A John Wiley & Sons, Ltd., Publication, pp. 9-11.

Molyneux, P. 2004. The use of stable free radical diphenylpicryl hydrazyl (DPPH( for estimating antioxidant activity. Songklanakarin Journal of Science and Technology, 26: 211-219.

Ordonez, A.A., Gomez, J.D., Vattuone, M.A., lsla, M.I. 2006. Antioxidant activities of Sechium edule (Jacq.) Swartz extracts. Food Chemistry, 97: 452-458.

Panicker, V.P., George, S. and Krishna, B.D. 2014. Toxicity study of butylated hydroxyl toluene (BHT) in rats. International Journal of Pharmacy and Pharmaceutical Sciences, 3: 758-763.

Petti, S. and Scully, C. 2009. Polyphenols, oral health and disease: A review.  Journal of Dentistry, 37: 413–423.

Pereira, D.M., Faria, J., Gaspar, L., Ferreres, F., Valentao, P., Sottomayor, M. and Andrade, P.B. 2010. Exploiting Catharanthus roseus roots: source of antioxidants. Journal of Food chemistry, 121: 56-61.

Rahmat, A., Kumar, V., Fong, L.M., Endrini, S. and Sani, H.A. 2003. Determination of total antioxidant activity in three types of local vegetables shoots and the cytotoxic effect of their ethanol extracts against different cancer cell lines. Journal of the Asia Pacific Clinical Nutrition, 12: 292-295.

Saha, K. Lajis, N.H., Israf, D.A., Hamzah, A.S., Khozirah, S., Khamis, S. and Syahida, A. 2004. Evaluation  of  antioxidant  and  nitric  oxide inhibitory  activities  of  selected  Malaysian  medicinal  plants.  Journal of Ethnopharmacology, 92(2-3): 263-267. 

Schillaci, C., Nepravishta, R. and Bellomaria, A. 2014. Antioxidants in food and pharmaceutical research. Albanian Journal of Pharmaceutical Sciences, 1: 15-25.

Singleton, V.L., Orthofer, R. and Lamuela, R. 1999. Analysis of total phenols and other oxidation substrates and antioxidants by means of Folin-Ciocalteu readent. Methods in Enzymology, 299: 152-178.

Stoilova, A., Krastano, A., Dtoyanova, P.,  Senev, P. and  Fova, S. 2007. Antioxidant activity of ginger extract Zingiber ficinale. Food Chemistry, 102: 764-770.

Wardle, E.N. 2005. Cellular oxidative proceses in realation to renal disease. American Journal of Nephrology, 25: 13-22.

Yu, L. 2001. Free radical scavenging properties of conjugated linoleic acids. Journal of Agricultural and Food Chemistry, 49: 3452-3456.