Antioxidant activity of methanol extract of Salvia aethiopis L. on circulatory system of rat

Subject Areas : Journal of Medicinal Herbs, "J. Med Herb" (Formerly known as Journal of Herbal Drugs or J. Herb Drug)

مصطفی  اسدبگی ¹ (گروه زیست شناسی، دانشکده علوم پایه، دانشگاه بوعلی سینا، همدان، ‏ایران؛)
رویا  کرمیان ² (گروه زیست شناسی، دانشکده علوم پایه، دانشگاه بوعلی سینا، همدان، ‏ایران؛)
پریسا  حسنین ³ (گروه زیست شناسی، دانشکده علوم پایه، دانشگاه بوعلی سینا، همدان، ‏ایران؛)
مسعود  رنجبر ⁴ (گروه زیست شناسی، دانشکده علوم پایه، دانشگاه بوعلی سینا، همدان، ‏ایران؛)
رامتین  پاکزاد ⁵ (گروه زیست شناسی، دانشکده علوم پایه، دانشگاه بوعلی سینا، همدان، ‏ایران؛)

Received: 2011-09-23 Accepted : 2011-11-11 Published : 2011-12-01

Keywords: Rat, antioxidant activity, DPPH free radical, Salvia aethiopis,

Abstract :

Background & Aim: Free radicals play a crucial role in the development of tissue damage in various human diseases such as cancer, aging, neurodegenerative disease, malaria and arteriosclerosis, and pathological events in living organisms. Antioxidants may have an important role in the prevention of these diseases. The aim of this study is to evaluate potency of scavenging of DPPH free radicals in the circulatory system of rats by the methanolic extracts of Salvia aethiopis. This species belongs to the genus Salvia of the family Lamiaceae, many species of which are valuable in food and medicine. Further studies will indicate whether this plant can be as a suitable candidate for pharmacological and industrial applications. Experimental: Ten female rats (200-250 g) were randomly selected and then divided in three groups. The rats were injected intraperitoneally with 0.5 ml of 1, 1-diphenyl-2-picryl hydrazyl (DPPH) free radical in methanol (3×10-4 M), 1 ml of methanolic extract of S. aethiopis (1 mg/ml) along with 0.5 ml of methanolic solution of DPPH and 1 ml of ascorbic acid along with 0.5 ml of methanolic solution of DPPH as positive control, respectively. The absorbance of DPPH solution in the blood serum of rats was measured by spectrophotometric method in 517 nm. Results & discussion: Decrease in absorbance of DPPH free radical (1.85 nm) in circulatory system of the rats in presence of ascorbic acid as positive control (1.07 nm) and S. aethiopis extract (0.78 nm) as sample. It can be concluded that the potency of free radical scavenging by the metabolic extract of S. aethiopis in circulatory system of the rats is more than that of ascorbic acid. Industrial and practical recommendations: The Results from present investigation showed that S. aethiopis extract can be used as a natural compound with a high antioxidant activity in future studies.

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