Effects of different medium on morphophysiological traits of Lippia citriodora H.B.K. during in vitro culture condition
Subject Areas : Journal of Medicinal Herbs, "J. Med Herb" (Formerly known as Journal of Herbal Drugs or J. Herb Drug)
حسن نورافکن
1
,
فاطمه سفیدکن
2
,
سیدامیر موسوی
3
,
مظفر شریفی
4
,
احمد خلیقی
5
1 - گروه علوم باغبانی، دانشکده کشاورزی، واحد علوم و تحقیقات، تهران، ایران؛
2 - موسسه تحقیقات جنگل ها و مراتع کشور، تهران، ایران؛
3 - پژوهشگاه ملی مهندسی ژنتیک و زیست فناوری، تهران، ایران
4 - گروه علوم گیاهی، دانشکده علوم زیستی، دانشگاه تربیت مدرس، تهران، ایران؛
5 - گروه علوم باغبانی، دانشکده کشاورزی، واحد علوم و تحقیقات، تهران، ایران؛
Keywords: Medicinal Plants, tissue culture, BAP, Lemon Verbena, Charcoal,
Abstract :
Background & Aim: Lemon verbena (Lippia citriodora H.B.K.) is a richly scented herb and has a long history as a sacred and medicinal plant. Select an appropriate medium in tissue culture is a necessity for success Experimental: To evaluate the effect of 10 medium of previous researches for Lippia micropropagation (1- MS medium supplemented with BAP (3 mgL-1) + IBA (0.1 mgL-1) + activated charcoal (1 gL-1) 2- MS medium supplemented with BAP (1 mgL-1) + IBA (0.5 mgL-1) + activated charcoal (1 gL-1) 3- 1/2MS 4- MS medium supplemented with BAP (5 mgL-1) 5- MS medium supplemented with BAP (1 mgL-1) 6- MS medium supplemented with activated charcoal (1 gL-1) 7- MS medium supplemented with BAP (1 mgL-1) + activated charcoal (1 gL-1) 8- MS medium supplemented with BAP (1 mgL-1) + AgNO3 (5 mgL-1) 9- MS medium supplemented with AgNO3 (5 mgL-1) 10- MS (control)), an experiment was conducted as a Completely Randomized Design (CRD) with three replicates and three explants per replication. Results & Discussion: The results showed positive effects for BAP (5 mgL-1) and somewhat active charcoal, but contrary to predictions, AgNO3 had no effect on reducing senescence and leaf necrosis. Recommended applications/industries: Therefore, Based on the finding, the four medium (MS medium supplemented with BAP (5 mgL-1)) with the highest rates of shoot fresh weight, leaf number, internodes number, shoot number, root fresh weight, callus diameter and root dry weight can be introduced as a suitable medium of tissue culture in this experimental conditions.
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