Antidiabetic and anti-oxidant effects of methanol leaf extract of Momordica charantia following alloxan-induced hyperglycaemia in rats

Subject Areas : Journal of Medicinal Herbs, "J. Med Herb" (Formerly known as Journal of Herbal Drugs or J. Herb Drug)

ساندی افوگ ¹ , الوفکه فلایی ² , بلسین اگونپلو ³ , آدمولا اویابمی ⁴ , تمیدایو اوموبوال ⁵ , مومو یکوبو ⁶ , اولووافمی اوگونتیبجو ⁷ , آدلو آدداپو ⁸

1 - گروه فارماکولوژی و سم شناسی دامپزشکی ، دانشگاه ابادان ، نیجریه؛
2 - گروه فارماکولوژی و سم شناسی دامپزشکی ، دانشگاه ابادان ، نیجریه؛
3 - گروه دامپزشکی ، دانشگاه ابادان ، نیجریه؛
4 - گروه فیزیولوژی دامپزشکی و بیوشیمی ، دانشگاه ابادان ، نیجریه؛
5 - گروه دامپزشکی ، دانشگاه ابادان ، نیجریه؛
6 - گروه علوم محیط زیست و علوم بین رشته ای ، COSET ، دانشگاه جنوبی تگزاس ، هیوستون ، TX ، ایالات متحده ؛
7 - گروه علوم زیست پزشکی ، دانشگاه صنعتی شبه جزیره کیپ ، بلزویل ، آفریقای جنوبی؛
8 - گروه فارماکولوژی و سم شناسی دامپزشکی ، دانشگاه ابادان ، نیجریه؛

Received: 2020-05-02 Accepted : 2020-08-03 Published : 2019-08-01

Keywords: Malondialdehyde, superoxide dismutase, Diabetes mellitus, Alloxan, hydrogen peroxide, Glutathione peroxidase, Momordica charantia,

Abstract :

Background & Aim:The plant Momordica charantia is highly valuable having a wide range of medicinal uses with tropical and subtropical regions distribution. This study was conducted to appraise the medicative properties of methanol leaf extract of Momordica charantia (MEMC) on alloxan-induced diabetic rats. Experimental: The antidiabetic influence of methanol leaf extract of Momordica charantia (MEMC) was investigated in alloxan-induced diabetes in rats. 50 rats allocated into five groups (A-E) (n =10) were utilized in this study: group A was normal control, groups B to E were induced with alloxan with diabetes established, while group B was not treated, group C received glibenclamide treatment while groups D and E were administered extracts at 200 and 400 mg/kg doses, respectively, in a study that continued for 28 days. Changes in blood glucose levels, body weight, haematology, serum chemistry, antioxidant system and histopathology changes were evaluated. Results: MEMC elicited significant drop in blood glucose level from diabetic to near normal level, restoring the body weight, haematological and serum biochemical parameters to the basal non diabetic level; likewise the MEMC-treated group elicited a decreased level of oxidant markers such as malondialdehyde (MDA) and hydrogen peroxide (H2O2) but increased concentration of protein thiols (PT), non-protein thiols (NPT), glutathione (GSH), glutathione peroxidase (GPx), glutathione-S-transferase (GST) and superoxide dismutase (SOD) indicating its anti-oxidant potential. The pancreas section also revealed repair of distorted pancreatic architecture in MEMC-treated group compared to diabetic group. Recommended applications/industries: The plant exhibited anti-hyperglycaemic, anti-diabetic and anti-oxidant abilities hence could be explored for the treatment of diabetes mellitus.

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