Comparative study of the effect of Eucalyptus extract on Candida albicans and human pathogenic bacteria
Subject Areas : Journal of Medicinal Herbs, "J. Med Herb" (Formerly known as Journal of Herbal Drugs or J. Herb Drug)
زهرا دهباشی
1
(
گروه زنان و زایمان، دانشکده پزشکی، دانشگاه علوم پزشکی زابل، زابل، ایران
)
فروغ فرقانی
2
(
گروه زنان و زایمان، دانشکده پزشکی، دانشگاه علوم پزشکی زابل، زابل، ایران
)
سید کاظم صباغ
3
(
گروه زیست شناسی، دانشکده علوم، دانشگاه یزد، یزد، ایران
)
سعیده سعیدی
4
(
کارشناس میکروبیولوژی، پژوهشکده زیست فناوری کشاورزی، دانشگاه زابل، زابل، ایران
)
Keywords: Minimum inhibitory concentration, Candida albicans, Anti-fungal activity, Anti-microbial activity, E. globules,
Abstract :
Background & Aim:During recent decades, infections disease resulting from opportunistic fungi such as Candida albicans and resistance increasing of human pathogenic bacteria to current antibiotics has led to many problems for treating of these diseases. The aims of the present study was to investigate anti-fungi and anti-bacterial effects of Eucalyptus extract on Candida albicans species isolated from clinical samples and some standard human pathogenic bacteria. Experimental: Eucalyptus extract was provided using rotary apparatus and maceration method. Thirty isolates of Candida albicans were isolated from referred patients to gynecologist and then were purified and identified by valid keys. Finally, 30 isolated were used to growth inhibitory activity assay. Minimum bactericidal concentration (MBC) and minimum inhibitory concentration (MIC) of eucalyptus extract against 5 standard bacteria inculding: Staphylococcus aureu, Shigella dysenteriae, Listeria monocytogenes, Vibrio cholera and Bacillus cereus were evaluated using micro broth dilution method. Results: Our results indicated that in compare to bacterial strains, fungus isolates showed more sensitivity to eucalyptus extract. The highest and lowest MIC of extract was recorded at 12.5 and 3.1 ppm for S. aureus and B. cereus, respectively. As MIC, the maximum MBC (20ppm) and minimum MBC (5ppm) of extract was recorded fro S. aureus and B. cereus respectively. Minimum inhibitory concentration of extract to fungal growth inhibitory was 50 ppm, whereas the maximum inhibiting concentration was 150 ppm. Recommended applications/industries: The positive and interesting results suggest the essential oil of E. globulus could be exploited antibiotic for the treatment of candidiasous disease caused by Candida albicans fungi, and some human pathogenic bacteria studied in this work.
Amber, K., Aijaz, A., Immaculata, X., Luqman, K., and Nikhat, M., 2010. Anticandidal effect of Ocimum sanctum essential oil and its synergy with fluconazole and ketoconazole. Phytomedicine. 17 )12): 921-925.
Bachir, R.G., and Benali, M., 2012. Antibacterial activity of the essential oils from the leaves of Eucalyptus globulus against Escherichia coli and Staphylococcus aureus. Asian Pacific Journal of Tropical Biomedicine. 2 )9): 739-742.
Baker, R.D., Angulo, O., Barroso-Tobila, C., Carbonell, L.M., Cespedes, R., Chick, E.W., Clark, B.M., Duque, O., Edington, G.M., and Fetter, B.F., 2012, The pathologic anatomy of mycoses: human infection with fungi, actinomycetes and algae, Springer Science & Business Media.
Biyela, P., Lin, J., and Bezuidenhout, C., 2004. The role of aquatic ecosystems as reservoirs of antibiotic resistant bacteria and antibiotic resistance genes. Water Science & Technology. 50 )1): 45-50.
Bokaeian, M., Nakhaee, A., Moodi, B., and Khazaei, H.A., 2010. Eucalyptus globulus (eucalyptus) treatment of candidiasis in normal and diabetic rats. Iranian Biomedical Journal. 14 )3): 121-129
Cates, W., Rolfs, R.T., and ARAL, S.O., 1990. Sexually transmitted diseases, pelvic inflammatory disease, and infertility: an epidemiologic update. Epidemiologic Reviews. 12 (1): 199-220.
Cermelli, C., Fabio, A., Fabio, G., and Quaglio, P., 2008. Effect of eucalyptus essential oil on respiratory bacteria and viruses. Current Microbiology. 56 (1): 89-92.
Consolaro, M.L., Albertoni, T.A., Yoshida, C.S., Mazucheli, J., Peralta, R.M., and Svidzinski, T.E., 2004. Correlation of Candida species and symptoms among patients with vulvovaginal candidiasis in Maringá, Paraná, Brazil. Review Iberoam Micology 21(4): 202-205.
De Vos, M., Cuenca‐Estrella, M., Boekhout, T., Theelen, B., Matthijs, N., Bauters, T., Nailis, H., Dhont, M., Rodriguez‐Tudela, J., and Nelis, H., 2005. Vulvovaginal candidiasis in a Flemish patient population. Clinical Microbiology and Infection. 11(12): 1005-1011.
Genani, F., Akbari, S., Delfan, B., Tolabi, T., Ebrahim zadeh, and Motamedi, M., 2011. Coamparsion study of effect of Myrtus communis vaginal -cream and clotrmazol on treatment of vaginal candisiosis Yafteh. 13(1): 35-43.
Ghalem, B.R., and Mohamed, B., 2008. Antibacterial activity of leaf essential oils of Eucalyptusglobulusand Eucalyptus camaldulensis. African Journal of Pharmacy and Pharmacology. 2(10): 211-215.
Ghorbani, F., Panjehkeh, N., Nasrollanejad, S., Salari, M., and Sabagh, S., 2014. Inhibitory effect of essential oils of Eucalyptus sp. and Zataria multiflora on fungal growth of Macrophomina phaseolina. Archives of Phytopathology and Plant Protection. 47(19): 2410-2413.
Gilles, M., Zhao, J., An, M., and Agboola, S., 2010. Chemical composition and antimicrobial properties of essential oils of three Australian Eucalyptus species. Food Chemistry. 119(2): 731-737.
Giraldo, P., von Nowaskonski, A., Gomes, F.A., Linhares, I., Neves, N.A., and Witkin, S.S., 2000. Vaginal colonization by Candida in asymptomatic women with and without a history of recurrent vulvovaginal candidiasis. Obstetrics & Gynecology. 95(3): 413-416.
Goswami, R., Dadhwal, V., Tejaswi, S., Datta, K., Paul, A., Haricharan, R., Banerjee, U., and Kochupillai, N., 2000. Species-specific prevalence of vaginal candidiasis among patients with diabetes mellitus and its relation to their glycaemic status. Journal of Infection. 41(2): 162-166.
Hammer, K.A., Carson, C., and Riley, T., 1999. Antimicrobial activity of essential oils and other plant extracts. Journal of Applied Microbiology. 86(5): 985-990.
Hay, R., and Ashbee, H., 2010. Mycology. Rook's Textbook of Dermatology, Eighth Edition.1-93.
Hoehamer, C.F., Cummings, E.D., Hilliard, G.M., and Rogers, P.D., 2010. Changes in the proteome of Candida albicans in response to azole, polyene, and echinocandin antifungal agents. Antimicrobial Agents and Chemotherapy. 54: 1655-1664.
Kottearachchi, N.S., Sammani, A., Kelaniyangoda, D.B., and Samarasekara, R., 2012. Anti-fungal activity of essentialoils of Ceylon Eucalyptus species for the control of Fusarium solaniandSclerotiumrolfsii. Phytopathology and Plant Protection. 45(3): 2026-2035.
Levy, S.B., 1998. The challenge of antibiotic resistance. Scientific American. 278: 32-39.
Maciel, M., Morais, S., Bevilaqua, C., Silva, R., Barros, R., Sousa, R., Sousa, L., Brito, E., and Souza-Neto, M., 2010. Chemical composition of Eucalyptus spp. essential oils and their insecticidal effects on Lutzomyia longipalpis. Veterinary Parasitology. 167(1): 1-7.
Mathema, B., Cross, E., Dun, E., Park, S., Bedell, J., Slade, B., Williams, M., Riley, L., Chaturvedi, V., and Perlin, D.S., 2001. Prevalence of vaginal colonization by drug-resistant Candida species in college-age women with previous exposure to over-the-counter azole antifungals. Clinical Infectious Diseases. 33(5): e23-e27.
Merenstein, D., Hu, H., Wang, C., Hamilton, P., Blackmon, M., Chen, H., Calderone, R., and Li, D., 2013. Colonization by Candida species of the oral and vaginal mucosa in HIV-infected and noninfected women. AIDS Research and Human Retroviruses. 29(1): 30-34.
Moosa, M.-Y.S., Sobel, J.D., Elhalis, H., Du, W., and Akins, R.A., 2004. Fungicidal Activity of Fluconazole against Candida albicans in a Synthetic Vagina-Simulative Medium. Antimicrobial Agents and Chemotherapy. 48(1): 161-167.
Neo, H., 2011. Worlds of food: Place, power, and provenance in the food chain. Asian Journal of Social Science. 39(3): 409-410.
Noumi, E., Snoussi, M., Hajlaoui, H., Trabelsi, N., Ksouri, R., Valentin, E., and Bakhrouf, A., 2011. Chemical composition, antioxidant and antifungal potential of Melaleuca alternifolia (tea tree) and Eucalyptus globulus essential oils against oral Candida species. Journal of Medicinal Plants Research. 5(17): 4147-4156.
Otang, W.M., Grierson, D.S., and Ndip, R., 2012. Ethnobotanical survey of medicinal plants used in the management of opportunistic fungal infections in HIV/AIDS patients in the Amathole District of the Eastern Cape Province, South Africa. Journal of Medicinal Plnat Research. 6 (11): 2071. 2080.
Patterson, T.F., Revankar, S.G., Kirkpatrick, W.R., Dib, O., Fothergill, A.W., Redding, S.W., Sutton, D.A., and Rinaldi, M.G., 1996. Simple method for detecting fluconazole-resistant yeasts with chromogenic agar. Journal of Clinical Microbiology. 34(7): 1794-1797.
Pinto, R.J., Marques, P.A., Neto, C.P., Trindade, T., Daina, S., and Sadocco, P., 2009. Antibacterial activity of nanocomposites of silver and bacterial or vegetable cellulosic fibers. Acta Biomaterialia. 5(6): 2279-2289.
Saeedi, S., SABBAGH, S., and SABORI, R.E., 2012. A Study of antibacterial activity of plant extract and essential oil of Myrtus communis against resistant strains of Staphyloccocus aureus bacteria to selective antibiotics. Rostamineh. 4(1): 21-32.
Saeeidi, S., Sabbagh, S.K., and Bazi, S., 2014. Antibacterila Effect of Eucalyptus globules Againts Resistance Staphyloccocus aureus. 5(4): 50-58.
Schuster, M.G., Edwards, J.E., Sobel, J.D., Darouiche, R.O., Karchmer, A.W., Hadley, S., Slotman, G., Panzer, H., Biswas, P., and Rex, J.H., 2008. Empirical fluconazole versus placebo for intensive care unit patients: a randomized trial. Annals of Internal Medicine. 149(2): 83-90.
Takahashi, E., 1966. Effect of silicon on resistance of rice to radiation. Jpn J Soil Sci Plant Nutr. 37: 183-188.
Takahashi, T., Kokubo, R., and Sakaino, M., 2004. Antimicrobial activities of eucalyptus leaf extracts and flavonoids from Eucalyptus maculata. Letters in Applied Microbiology. 39(1): 60-64.
Wilson, C., Solar, J., El Ghaouth, A., and Wisniewski, M., 1997. Rapid evaluation of plant extracts and essential oils for antifungal activity against Botrytis cinerea. Plant Disease. 81(2): 204-210.