α-Amylase inhibition and antioxidant activity of Singhnad Guggul in vitro

Subject Areas : Journal of Medicinal Herbs, "J. Med Herb" (Formerly known as Journal of Herbal Drugs or J. Herb Drug)

اشوک کومار ¹ , سالیمولا خان ² , گوپی ستی ساران ³ , ناوین کومار ⁴ , لوکانت وی ⁵

1 - گروه فارماکوگنوزی، سری کا وی کالج داروسازی، چیکبالاپور، کاراناتاکا، هند؛
2 - گروه فارماکوگنوزی، کالج داروسازی مانیپال، مانیپال، کاراناتاکا، هند؛
3 - گروه فارماکوگنوزی، سری کا وی کالج داروسازی، چیکبالاپور، کاراناتاکا، هند؛
4 - گروه فارماکوگنوزی، سری کا وی کالج داروسازی، چیکبالاپور، کاراناتاکا، هند؛
5 - گروه فارماکوگنوزی، سری کا وی کالج داروسازی، چیکبالاپور، کاراناتاکا، هند؛

Received: 2012-10-11 Accepted : 2012-12-27 Published : 2013-07-01

Keywords: &alpha, -amylase, Singhnad Guggul, Chelating assay,

Abstract :

Background & Aim: Singhnad Guggulu is a traditional Ayurvedic formula for detoxifying and rejuvenating the joints. The present study was to investigate the α-amylase inhibition and antioxidant activities of aqueous extract of Singhnad Guggul (AESG). Experimental: α-Amylase inhibition assay by dinitro-salicylic acid method and antioxidant activity by ferric chelating and ferrous reducing assay. Results: AESG indicated that potent α-amylase inhibition activity (IC50= 84.00 µg/ml). AESG also showed ferric chelating and ferrous reducing activities. Recommended applications/industries: Our studies showed that Singhnad Guggulu possess alpha- amylase and antioxidant properties.

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