A study of UV-C and plant growth regulators effects on plantlets of Aloe vera Line.

Subject Areas : Journal of Medicinal Herbs, "J. Med Herb" (Formerly known as Journal of Herbal Drugs or J. Herb Drug)

نسترن صادقی ¹ , مرضیه شفیعی حاجی آباد ² , امیر مهدی شوکتی ³

1 - عضو هیئت علمی دانشگاه آزاد اسلامی واحد کاشمر ( دانشجوی دکتری فیزیک- دانشگاه آزاد اسلامی واحد علوم تحقیقات) ؛
2 - دانشجوی دکتری گیاهان دارویی، دانشگاه گیسن، ایالت هسن، آلمان
3 - کارشناس گیاهان دارویی، دانشگاه آزاد اسلامی واحد کاشمر ؛

Received: 2010-04-15 Accepted : 2010-06-01 Published : 2010-08-01

Keywords: In vitro culture, IBA, BA, Aloe vera L, Ultraviolet C,

Abstract :

Background & Aim: Effects of ultra violet spectrum on plants due to their obligatory requirement for sunlight for survival are unavoidable. Direct effects of UV on plant growth and development are generally negative and usually they use many different defense mechanisms to acclimate and protect themselves.Material & Methods: The present research for the first time was conducted to investigate the destructive, target or even possible constructive effects of ultraviolet-C (100-280 nm) on in vitro plantlets of Aloe vera and changes of these effects under different concentrations of plant growth regulators of media cultures. Aloe was selected because it is one of the most important subtropical medicinal plants and normally it is exposed to UV in its original growing regions. Treatments were media culture in four stages and UV-C in two stages (0 and 40 Mwcm-1). In vitro plants were cultured on four different media in six replications. Three replications of them were exposed one hour per day with UV-C for one month.Results & Discussion : Our results showed that longest plantlets, highest number of roots and longest roots formed on the media contain 1.5 mgl-1IBA and highest number of shoots on the media contain 1.5 mgl-1IBA and 1 mgl-1 BA.Conclusion: UV-C has significantly reduced the length of roots and shoots root number but increased the shoot number.

References:

انتشاری، ش.، منوچهری کلانتری، خ.، قربانلی ، م و ترک زاده ، م. 1384. تأثیر باندهای مختلف اشعه­ی ماورای بنفش بر رنگیزه‌های موجود در برگ سویا (Glycine max L.).
 فصل­نامه­ی زیست­شناسی ایران، 18: 77 -83 .

باقری ، ع و صفّاری، م. 1376. مبانی کشت بافت‌های گیاهی . انتشارات دانشگاه فردوسی مشهد، 406 صفحه.

بلوچی ، ح.، مدرّس ثانوی ، ع.م.، امام ، ی و برزگر ، م. 1387. تأثیر تنش کم­آبی ، ازدیاد دی‌اکسید کربن و اشعه­ی ماورای بنفش بر صفات کیفی برگ پرچم گندم دوروم (Triticum turgidum L. var durum Desf)، مجله­ی علوم و فنون کشاورزی و منابع طبیعی, 12: 167-181.

قناتی ، ف.، احمدی، ز و عبدالمالکی، پ. 1385. تأثیر پرتوی فرابنفش C بر برخی پارامترهای فیزیولوژیکی در گیاه صبر زرد ( Aloe vera)، فصل­نامه­ی علمی پژوهشی تحقیقات گیاهان دارویی و معطر ایران 22: 315-331.

مهدویان، ا.، قربانلی، م.، منوچهری کلانتری، خ و محمدی، غ. 1385. تأثیر باندهای مختلف اشعه­ی ماورای بنفش بر عوامل فیزیولوژیکی و ریخت­شناسی فلفل (Capsicum annuum L.). فصل­نامه­ی زیست­شناسی ایران، 19: 43-48.

Abrie, A. and Staden, J.V. 2001. Micropropagation of endangered Aloe Polyphylla. J. Plant Growth  Regul, 33 (1): 19-23.

Ahmed, S., Kabir, A. H., Ahmed, M. B., Razvy, M. A. and Ganesan, S. 2007. Development of rapid micropropagation method of Aloe vera L. Sjemenarstvo 24: 121-128.

Alagukannan, G., Ganesh, S. and Gopal, S. K. 2002. Characterization and screening of different ecotypes of Aloe vera L. for growth, yield and quality, Submitted for Yun-Ho Award, International Aloe Science Council, Texas. Pp: 48.

Arteca, R. N. 1995. Plant Growth Regulators, Substances Principles and Applications. Chapman and Hall, New York. PP: 332.

Ballare, C. L., Baness, P. W. and Flint, S. D. 1995. Inhibition of hypocotyls elongation by ultrsviolet-B radiation in de-etiolating tomato seedlings. Photoreceptor. Physiol. Plant, 93: 548-592.

Blackburn, G. A. 1998. Spectral indices for estimating photosynthetic pigment concentrations: a test using senescent tree leaves. Int J Remote Sens, 19: 657 – 675.

Chaudhuri, S. and Mukundan, U. 2001. Aloe vera L. - Micropropagation and Characterization of its gel. Phytomorphology, 51 (2): 155-157.

Ehsanpour, A. A. and Razavizadeh, R. 2005. Effect of UV-C on Drought Tolerance of Alfalfa (Medicago sativa) Callus. Am J Biochem Biotechnol, 1 (2): 107-110.

Farokh, P., Mohmoodzadeh, H. and Satari, T. N. 2010. Response of seed germination of sunflower to UV-B radiation. Res J. Environ Sci, 4(1): 70-74.

Fattahi, M. J., Oghli, Y. H. and Ghazvini, R. F. 2004. Introduction of the most suitable culture media for micropropagation of a medicinal plant aloe (Aloe barbadensis Mill.). Iranian J. Hortic Tech Sci, 5: 71-80. 

Filella, I., Serrano, J., Serra. and Penuelas, J. 1995. Evaluating wheat nitrogen status with canopy reflectance indices and discriminant analysis. Crop Sci, 35: 1400-1405.

Gamon, J. A. and Surfus, J. S. 1999. Assessing leaf pigment content and activity with a reflectometer. New Phytologist 143: 105 – 117.

Hashemabadi, D. and Kaviani, B. 2008. Rapid micro-propagation of Aloe vera L. via shoot multiplication. Afric J. Biotech, 7 (12): 1899-1902.

Hirimburegama, K. and Gamage, N. 1995. In vitro multiplication of Aloe vera meristem tips for mass propagation. Hortic Sci, 27 (3-4): 15-18.

Jansen, M. A. K. 2002. Ultraviolet- B radiation effects on plants: Induction of morphogenic responses. Physiol Planta, 116: 423-429.

Kakanni, V. G., Reddyer, K. R., Zhao, D. and Mohammad, A. R. 2003. Effects of Ultraviolet-B radiation on cotton (Gossypium hirsutum L.). Morphol. Anatomy Annal Bot, 91: 817-826.

Krizek, D. T., Kramer, G. F., Upadhyaya. A. and Mirecki., R. M. 1993. UV-B response of cucumber seedling grown under metal halide and high pressure sodium/deluxe lamps. Physiol Plantarum, 88: 350-358.

Manetas, Y., Petropoulou, Y., Stamatakis, K., Nikolopoulos, D., Levizou, E., Psaras, G. and Karabourniotis, G. 1997. Beneficial effects of enhanced UV-B radiation under field conditions: improvement of needle water relations and survival capacity of Pinus pinea L. seedlings during the dry Mediterranean summer. Plant Ecol, 128: 100-108.

Meyer, H. J. and Staden, J. V. 1991. Rapid in vitro propagation of Aloe barbadensis Mill. Plant Cell, Tis Org Cul, 26 (3): 167-171.

Mpoloka, S. W., Abratt, V. A, Mundree, S. G., Thomson, J. A. and Musil C. F. 2007. Potential effects of prolonged ultraviolet radiation exposure in plants: chloroplast DNA analysis. Am-Eurasian J. Agric.  Environ Sci, 2(4): 437-441.

Mukherjee, A. and Roy-Chowdhury, B. 2008. The In Vitro Propagation of Aloe vera sp. TIG Res J, 1 (2): 116-119.

Natali, L., Sanchez, I. C. and Cavallini, A. 1990. In vitro culture of Aloe barbadensis Mill. micropropagation from vegetative meristems. Plant Cell, Tis Org Cul, 20: 71-74.

Normanly, J. 1997. Auxin metabolism. Physiol. Plant. 100: 422-431

Ormrod, D. P. and Hale, B. A. 2000. Physiological responses of plants and crops to ultraviolet-B radiation stress. Air Pollution, 761-770.

Paez, A., Gebre, G. M., Gonzales, M. E. and Tschslinski, T. J. 2000. Growth, soluble carbohydrates and aloin concentration of Aloe vera plants exposed to tree iradiance levels environmental and experimental botany levels. Environ. Exp. Bot, 44: 133-139.

Rahmaatzadeh, S. and Khara, J. 2007. Anatomical and morphological changes caused by intraction between UV-C Radiation and colonized wheat by some species of Mycorrhiza arbuscular. J. Bio. Sci, 7(6): 1001-1004.

Ros, J. and Tevini, M. 1995. Interaction of UV radiation and IAA during growth of seedling and hypocotyls segments of Sunflower. Plant Physiol, 146: 295-302.

Roy, S. C. and Sarkar, A. 1991. In vitro regeneration and micropropagation of Aloe vera L. Sci Hor, 47(1-2): 107-113.

Schreiner, M., Krumbein, A., Mewis, I., Ulrichs., C. and Huyskens-Keil, S. 2009. Short-term and moderate UV-B radiation effects on secondary plant metabolism in different organs of nasturtium (Tropaeolum majus L.). Innov Food Sci Emerg Tech, 10(1): 93-96.

Sembiring, H., Raun, W.R., Johnson, G.V., Stone, M.L., Solie, J. B. and Phillips, S.B. 1998. Detection of nitrogen and phosophorus nutrient status in winter wheat using spectral radiance. J. Plant Nut, 21 (6):1207-1233.

Small wood, M.F., Calvert, C.M., and Bowles, D.J. 1999. Plant Responses to Environmental stress. Annals of Bot, 85(4): 578-580

Stapleton, A.E. 1992. Ultraviolet radiation and plants: Burning questions. The Plant Cell, 4: 1353-1358.

Strid, A., Chow, W. S., Anderson, J. M. 1994. UV-B damage and protection at the molecular level in plants. Photosynth Res, 39: 475-489.

Tanabe, M. J. and Horiuchi, K. 2006. Aloe barbadensis Mill. ex vitro autotrophic culture. Journal of Hawahan Pacific Agriculture, 13: 55-59.

Velcheva M., Faltin Z., Vardi A., Eshdat Y. and Peral, A. 2005. Regeneration of Aloe arborescens via organogenesis from young inflorescences. Plant Cell, Tis Org Cul, 83: 293-301.

Wood, C. W., Reeves, D. W., Duffield, R. R. and Edmisten, K. L. 1992. Field chlorophyll measurements for evaluation of corn nitrogen status. J Plant Nutr, 15:487-500.

Ziska, L. H., Teramura, A. H., Sullivan, J. H. and McCoy, A. 1993. Influence of ultraviolet-B (UV-B) radiation on photosynthetic and growth characteristics in field-grown cassava (Manihot esculentum Crantz). Plant. Cell Environ, 16: 73-79.