The phytochemical response of Melissa officinalis L to foliar application of hydro-alcoholic solutions (methanol and ethanol)

Subject Areas : Journal of Medicinal Herbs, "J. Med Herb" (Formerly known as Journal of Herbal Drugs or J. Herb Drug)

اسماعیل خسروی ¹ , علی مهرآفرین ² , حسنعلی نقدی بادی ³ , رضا حاجی آقایی ⁴ , محمدتقی خسروی ⁵

1 - دانشگاه آزاد اسلامی واحد کرج، کرج، ایران؛
2 - گروه کشت وتوسعه پژوهشکده گیاهان دارویی جهاد دانشگاهی (ACECR)، کرج، ایران؛
3 - گروه کشت وتوسعه پژوهشکده گیاهان دارویی جهاد دانشگاهی (ACECR)، کرج، ایران؛
4 - گروه کشت وتوسعه پژوهشکده گیاهان دارویی جهاد دانشگاهی (ACECR)، کرج، ایران؛
5 - دانشگاه آزاد اسلامی واحد کرج، کرج، ایران؛

Received: 2011-08-03 Accepted : 2011-10-12 Published : 2012-04-01

Keywords: Methanol, ethanol, Quantity, volatile oil, Melissa officinalis L,

Abstract :

Introduction & Aim: The aim of this study was evaluating effect of methanol and ethanol foliar application as a new and safe technology in plant production on lemon balm’s (Melissa officinalis L.) volatile oil components.Experimental: Experimental carried out based on a Randomized Complete Block Design (RCBD) with fifteen treatments and three replications in the experimental field of medicinal plants institute (ACECR) in Haljerd in the Alborz province at 2010. The treatments of this study including: control, control distilled water, aqueous ethanol solutions with 10, 20, 30, 40 and 50% (v/v), aqueous methanol solutions with 10, 20, 30, 40 and 50% (v/v) and ethanol-methanol equal mixtures with 5, 15 and 25% (v/v). In this study, phytochemical characters by GC-MS were evaluated.Results & Discussion: The results were indicated that spraying of ethanol and methanol on phytochemical characters have significant difference (p<0.01). The most of biosynthesis of β-caryophyllene, neral, geranial, caryophyllene oxide, Δ-cadinene, Δ-cadinol, citronellal, germacrene-B and geranyl acetate were affected by methanol 50% solution.Industrial and practical Recommendations: The results showed that spraying with methanol 50% was the best treatment for increasing of volatile oil components of Melissa officinalis L. in Karaj conditions.

References:

امید بیگی، ر. 1388. تولید و فرآوری گیاهان دارویی، جلد اول، مشهد: انتشارات آستان قدس رضوی.

امیدبیگی، ر. 1387. تولید و فرآوری گیاهان دارویی، جلد سوم، مشهد: انتشارات آستان قدس رضوی.

بیگم فقیر، م. 1384. گیاهان آرایشی بهداشتی، گیلان: انتشارات دانشگاه گیلان.

رجحان، م. 1377. درمان بوسیله گیاهان دارویی، تهران: انتشارات مرکز فرهنگی آبا.

هی، ر و واترمن، پ. 1379. گیاهان اسانس دار، تهران: نشر اندرز.

 

Andreas, A. R., Lazaro, J. J., Chueca, A., Hermoso, R. and Lopez Gorge, J. 1990. Effect of alcohols on the association of photosynthetic FBPase to thylakoid membrances. Physiology Plant., 78: 409-413.

Heins, R. 1980. Inhibition of ethylene synthesis is and senescence incarnation by ethanol. Journal of American Society Horticulture. Science., 105: 141-144.

Morales, J. P. and Santos, B.M. 1997. Effects of different ethanol concentration on the initial growth of lettuce (Lactuca sativa). Proceeding of the Caribbean Food Crop Society., 33: 442-447.

Nonomura, A. and Benson, A. 1992. The path of carbon photosynthesis improved crop yields with methanol. Proceeding National Academic Science USA., 15: 9794-9798.