Crinum glaucum A. chev bulb: Modulation of endogenous antioxidant enzyme in an animal-induced oxidative stress model

Subject Areas : Journal of Medicinal Herbs, "J. Med Herb" (Formerly known as Journal of Herbal Drugs or J. Herb Drug)

اولوفون میلایو  اوگونرینولا ¹ (گروه بیوشیمی، دانشکده علوم، دانشگاه ایالتی لاگوس، لاگوس، نیجریه.)
اولواسی  اوگونرینولا ² (گروه بیوشیمی، دانشکده علوم، دانشگاه ایالتی لاگوس، لاگوس، نیجریه.|دانشکده علوم تغذیه، دانشکده علوم بهداشت، دانشگاه اتاوا، اتاوا، انتاریو، کانادا.)
اولواتومیلولا  اولاتونجی ³ (گروه بیوشیمی، دانشکده علوم، دانشگاه ایالتی لاگوس، لاگوس، نیجریه.)
اولوسگون  فاجانا ⁴ (گروه بیوشیمی، دانشکده علوم، دانشگاه ایالتی لاگوس، لاگوس، نیجریه.)
موتیو  کاظیم ⁵ (گروه بیوشیمی، دانشکده علوم، دانشگاه ایالتی لاگوس، لاگوس، نیجریه.)
ژمیسولا  سایبو ⁶ (گروه بیوشیمی، دانشکده علوم، دانشگاه ایالتی لاگوس، لاگوس، نیجریه.)
حبیب  بانکول ⁷ (گروه بیوشیمی، دانشکده علوم، دانشگاه ایالتی لاگوس، لاگوس، نیجریه.)
آدمولا  آدیوی ⁸ (گروه بیوشیمی، دانشکده علوم، دانشگاه ایالتی لاگوس، لاگوس، نیجریه.)
باباجید  المو ⁹ (گروه بیوشیمی، دانشکده علوم، دانشگاه ایالتی لاگوس، لاگوس، نیجریه.)

Received: 2023-05-18 Accepted : 2023-08-27 Published : 2023-11-22

Keywords: Catalase, superoxide dismutase, Lipopolysaccharide, Glutathione-S-transferase,

Abstract :

Background & Aim: The mechanism of action of Crinum glaucumbulb (Cgb) A. Chev (Amaryllidaceae) is enigmatic. Therefore, this study aimed at investigating the possible modulating properties of an aqueous extract of Cgb on endogenous antioxidant enzymes during lipopolysaccharide (LPS)-induced oxidative stress in a rat model.Experimental: 25 male and 25 female rats were divided into five groups (n=5) each: control group; treatment group, rats were given an aqueous extract of Cgb for 7 days; induced-oxidative stress group, rats were injected with LPS for 4 hours; post-Cgb group, rats were injected with LPS for 4 hours and treated with an aqueous extract of Cgb; and pre-Cgb group, rats were given an aqueous extract of Cgb for 7 days, injected with LPS for 4 hours, and treated with an aqueous extract of Cgb for 7 days. The blood, brain, heart, lungs, liver, and kidneys were harvested for the biochemical analysis. The endogenous antioxidant enzymes (catalase, superoxide dismutase, and glutathione-S-transferase) were analysed spectrophotometrically.Results: The hallmark of LPS is its ability to decrease the activity of oxidative stress marker enzymes, as observed in this study. The pre- and post-administration of an aqueous extract of Cgb significantly (P≤0.05) reversed the damaging effect of LPS by increasing the activities of catalase and superoxide dismutase in the blood and organs of male and female rats, respectively, while plasma glutathione-S-transferase activity was inhibited.Recommended applications/industries: The aqueous extract of Cgb has modulating properties to reduce the action of LPS-induced oxidative stress on endogenous antioxidant enzymes in male and female rats.

References:

Adu, O.B., Adeyemo, G.A., Falua, O.B., Fajana, O.O., Ogunrinola, O.O., Saibu, G.M. and Elemo, B.O. 2021. The effect of Thaumatococcus danielli leaf extracts on immunological and oxidative stress markers in rat. Asian Journal of Biochemistry, Genetics and Molecular Biology, 7:6-14.

Ajuwon, O.R., Oguntibeju, O.O. and Marnewick, J.L. 2014. Amelioration of lipopolysaccharide-induced liver injury by aqueous rooibos (Aspalathus linearis) extract via inhibition of pro-inflammatory cytokines and oxidative stress.BMC Complementary Alternative Medicine, 14(1):1-2.

Akintola, A.O., Kehinde, A.O., Adebiyi, O.E. and Ademowo, O.G. 2013. Anti-tuberculosis activities of the crude methanolic extract and purified fractions of the bulb of Crinum jagus. Nigerian Journal Physiological Sciences, 28(2):135-140.

Alam, M.N., Bristi, N.J. and Rafiquzzaman, M. 2013. Review on in vivo and in vitro methods evaluation of antioxidant activity. Saudi Pharmaceutical Journal, 21(2):143-152.

Al-Rikabi, R., Al-Shmgani, H., Dewir, Y.H. and El-Hendawy, S. 2020. In vivo and in vitro evaluation of the protective effects of hesperidin in lipopolysaccharide-induced inflammation and cytotoxicity of cell. Molecules, 25(3):478.

Alwaleedi, S.A. 2016. Alterations in antioxidant defense system in hepatic and renal tissues of rats following aspartame intake. Journal of Applied Biology and Biotechnology, 4(2): 46-52.

Apanasets, O., Grou, C.P., Van Veldhoven, P.P., Brees, C., Wang, B., Nordgren, M., Dodt, G., Azevedo, J.E. and Fransen, M. 2014. PEX5, the shuttling import receptor for peroxisomal matrix proteins, is a redox-sensitive protein. Traffic, 15(1): 94-103.

Arab, Z., Hosseini, M., Mashayekhi, F. and Anaeigoudari, A. 2020. Zataria multiflora extract reverses lipopolysaccharide-induced anxiety and depression behaviors in rats. Avicenna Journal of Phytomedicine, 10(1): 78-83.

Battelli, M.G., Bolognesi, A. and Polito, L. 2014. Pathophysiology of circulating xanthine oxidoreductase: New emerging roles for a multi-tasking enzyme. Biochimica et Biophysica Acta (BBA)-Molecular Basis of Disease, 1842(9): 1502-1517.

Battelli, M.G., Polito, L., Bortolotti, M. and Bolognesi, A. 2016. Xanthine oxidoreductase-derived reactive species: physiological and pathological effects. Oxidative Medicine and Cellular Longevity, 2016. Article ID 3527579.

Bertani, B. and Ruiz, N. 2018. Function and biogenesis of lipopolysaccharides. EcoSal Plus, 8(1):10-15.

Cahlíková, L., Vaněčková, N., Šafratová, M., Breiterová, K., Blunden, G., Hulcová, D. and Opletal, L. 2019. The genus Nerine Herb. (Amaryllidaceae): Ethnobotany, phytochemistry, and biological activity. Molecules, 24(23): 4238.

Donald, K.K.B., Irié-N’guessan Amenan Geneviève, E., Etienne, K. and Felix, Y.H. 2018. Evaluation of anti-inflammatory activity of Crinum scillifolium extracts in Wistar rats. Cardiovascular diseases, 6(4): 77-82.

Erhirhie, E.O., Ihekwereme, C.P. and Ilodigwe, E.E. 2018. Advances in acute toxicity testing: strengths, weaknesses and regulatory acceptance. Interdisciplinary toxicology, 11(1): 5-12.

Fernando, S.M., Rochwerg, B. and Seely, A.J. 2018. Clinical implications of the third international consensus definitions for sepsis and septic shock (Sepsis-3). Cmaj, 190(36): E1058-E1059.

Fuentes, F., Paredes-Gonzalez, X. and Kong, A.N.T. 2015. Dietary glucosinolates sulforaphane, phenethyl isothiocyanate, indole-3-carbinol/3, 3′-diindolylmethane: Antioxidative stress/inflammation, Nrf2, epigenetics/epigenomics and in vivo cancer chemopreventive efficacy. Current Pharmacology Reports, 1: 179-196.

Fujiki, Y., Abe, Y., Imoto, Y., Tanaka, A.J., Okumoto, K., Honsho, M., Tamura, S., Miyata, N., Yamashita, T., Chung, W.K. and Kuroiwa, T. 2020. Recent insights into peroxisome biogenesis and associated diseases. Journal of Cell Science, 133(9): jcs236943.

Fujiki, Y., Miyata, N., Mukai, S., Okumoto, K. and Cheng, E.H. 2017. BAK regulates catalase release from peroxisomes. Molecular & Cellular Oncology, 4(3): e1306610.

Guide for the care and use of laboratory animals. NIH Publication. 1985;85–23. Revised.1985.

Halawa, A.A., El-Adl, M.A., Hamed, M.F., Balboula, A.Z. and Elmetwally, M.A. 2018. Lipopolysaccharide prompts oxidative stress and apoptosis in rats’ testicular tissue. Journal of Veterinary. Health, 1: 20-31.

He, L., He, T., Farrar, S., Ji, L., Liu, T. and Ma, X. 2017. Antioxidants maintain cellular redox homeostasis by elimination of reactive oxygen species. Cellular Physiology and Biochemistry, 44(2): 532-553.

Hosoi, K.I., Miyata, N., Mukai, S., Furuki, S., Okumoto, K., Cheng, E.H. and Fujiki, Y. 2017. The VDAC2–BAK axis regulates peroxisomal membrane permeability. Journal of Cell Biology, 216(3): 709-722.

Hou, X., Zhang, J., Ahmad, H., Zhang, H., Xu, Z. and Wang, T. 2014. Evaluation of antioxidant activities of ampelopsin and its protective effect in lipopolysaccharide-induced oxidative stress piglets. PloS one, 9(9): e108314.

Houghton, P.J., Agbedahunsi, J.M. and Adegbulugbe, A. 2004. Choline esterase inhibitory properties of alkaloids from two Nigerian Crinum species. Phytochemistry, 65(21): 2893-2896.

Ishola, I.O., Olayemi, S.O. and Idowu, A.R. 2013. Anticonvulsant, anxiolytic and hypnotic effects of aqueous bulb extract of Crinum glaucum A. chev (Amaryllidaceae): Role of GABAergic and nitrergic systems. Pakistan Journal of Biological Sciences: PJBS, 16(15): 701-710.

Ji, M., Deng, Z., Rong, X., Li, R., You, Z., Guo, X., Cai, C., Zhao, Y., Gao, P., Cao, G. and Li, B. 2023. Naringenin Prevents Oxidative Stress and Inflammation in LPS-Induced Liver Injury through the Regulation of LncRNA-mRNA in Male Mice. Molecules, 28(1): 198-103.

Kolac, U.K., Ustuner, M.C., Tekin, N., Ustuner, D., Colak, E. and Entok, E. 2017. The anti-inflammatory and antioxidant effects of Salvia officinalis on lipopolysaccharide-induced inflammation in rats. Journal of Medicinal Food, 20(12): 1193-1200.

Kurutas, E.B. 2015. The importance of antioxidants which play the role in cellular response against oxidative/nitrosative stress: current state. Nutrition Journal, 15(1): 1-22.

Liu, Y., Li, Z., Xue, X., Wang, Y., Zhang, Y. and Wang, J. 2018. Apigenin reverses lung injury and immunotoxicity in paraquat-treated mice. International Immunopharmacology, 65: 531-538.

Ma, J., Xu, X., Wang, R., Yan, H., Yao, H., Zhang, H., Jiang, S. and Xu, A. 2020. Lipopolysaccharide exposure induces oxidative damage in Caenorhabditis elegans: Protective effects of carnosine. BMC Pharmacology and Toxicology, 21(1): 1-9.

Mohammad, S. and Thiemermann, C. 2021. Role of metabolic endotoxemia in systemic inflammation and potential interventions. Frontiers in immunology, 11: 594150.

Myburgh, K.H. 2014. Polyphenol supplementation: Benefits for exercise performance or oxidative stress? Sports Medicine, 44: 57-70.

Nakai, K. and Tsuruta, D. 2021. What are reactive oxygen species, free radicals, and oxidative stress in skin diseases? International Journal of Molecular Sciences, 22(19): 10799.

Nandi, A., Yan, L.J., Jana, C.K. and Das, N. 2019. Role of catalase in oxidative stress-and age-associated degenerative diseases. Oxidative Medicine and Cellular Longevity, 11: 2019.

Noworyta-Sokołowska, K., Górska, A. and Gołembiowska, K. 2013. LPS-induced oxidative stress and inflammatory reaction in the rat striatum. Pharmacological Reports, 65(4): 863-869.

Ogunrinola, O.O., Kanmodi, R.I., Adeyemo, G.A., Ogunrinola, O.A., Olatunji, K.O., Fayemi, A.O., Sunday, A.J., Adu, O.B. and Elemo, B.O. 2022a. Crinum glaucum Bulb Extract Improves the Lipid Profile of Endotoxin-Induced Wistar Rats. Asian Journal of Research in Biochemistry, 11(2): 10-17.

Ogunrinola, O.O., Kanmodi, R.I., Ogunrinola, O.A., Adegbulugbe, J.A., Adu, O.B. 2022b. Cholesterol and triglycerides concentrations of lipopolysaccharide-induced inflammatory male rat in response to Petiveria alliacea L. Leaf Extract. Journal of Applied Sciences, 22(2):100-106.

Ogunrinola, O.O., Wusu, D.A., Fajana, O.O., Olaitan, S.N., Smith, Z.O. and Bolaji, A.R.I. 2016. Effect of low level cadmium exposure on superoxide dismutase activity in rat. Tropical Journal of Pharmaceutical Research, 15(1): 115-119.

Olatunji K.O., Ogunrinola O.A., Ogunrinola O.O., Igbalaye O.J., Adeniyi A.E., Ahmad A.A., Aribisala B.S., Elemo B.O. 2023. Therapeutic Role of Crinum glaucum Bulb against Haematological and Hepatic Enzyme Alterations in a Rat Model. Asian Journal of Biochemistry, Genetics and Molecular Biology, 14(2):12-22.

Pizzino, G., Irrera, N., Cucinotta, M., Pallio, G., Mannino, F., Arcoraci, V., Squadrito, F., Altavilla, D. and Bitto, A. 2017. Oxidative stress: harms and benefits for human health. Oxidative Medicine and Cellular Longevity, 2017: Article ID 8416763.

Rahal, A., Kumar, A., Singh, V., Yadav, B., Tiwari, R., Chakraborty, S. and Dhama, K. 2014. Oxidative stress, prooxidants, and antioxidants: The interplay. BioMed Research International, 2014. Article ID 761264.

Refaat, J., Kamel, M.S., Ramadan, M.A. and Ali, A.A. 2013. Crinum; an endless source of bioactive principles: A review. Part V. Biological profile. International Journal of Pharmaceutical Sciences and Research, 4(4): 1239.

Salehi, B., Azzini, E., Zucca, P., Maria Varoni, E., V. Anil Kumar, N., Dini, L., Panzarini, E., Rajkovic, J., Valere Tsouh Fokou, P., Peluso, I. and Prakash Mishra, A. 2020. Plant-derived bioactives and oxidative stress-related disorders: A key trend towards healthy aging and longevity promotion. Applied Sciences, 10(3): 947-951.

Sebai, H., Sani, M., Yacoubi, M.T., Aouani, E., Ghanem-Boughanmi, N. and Ben-Attia, M. 2010. Resveratrol, a red wine polyphenol, attenuates lipopolysaccharide-induced oxidative stress in rat liver. Ecotoxicology and Environmental Safety, 73(5): 1078-1083.

Sharifi-Rad, M., Anil Kumar, N.V., Zucca, P., Varoni, E.M., Dini, L., Panzarini, E., Rajkovic, J., Tsouh Fokou, P.V., Azzini, E., Peluso, I. and Prakash Mishra, A. 2020. Lifestyle, oxidative stress, and antioxidants: Back and forth in the pathophysiology of chronic diseases. Frontiers in Physiology, 11: 694-698.

Sies, H. 2015. Oxidative stress: a concept in redox biology and medicine. Redox Biology, 4: 180-183.

Skibska, B., Goraca, A., Skibska, A. and Stanczak, A. 2022. Effect of alpha-lipoic acid on rat ventricles and atria under LPS-induced oxidative stress. Antioxidants, 11(4): 734.

Stefanov, G., Briyal, S., Pais, G., Puppala, B. and Gulati, A. 2020. Relationship between oxidative stress markers and endothelin-1 levels in newborns of different gestational ages. Frontiers in Pediatrics, 8: 279.

Stepanić, V., Trošelj, K.G., Lučić, B., Marković, Z. and Amić, D. 2013. Bond dissociation free energy as a general parameter for flavonoid radical scavenging activity. Food Chemistry, 141(2): 1562-1570.

Tian, H., Liu, Q.J., Wang, J.T. and Zhang, L. 2021. Antimicrobial crinane-type alkaloids from the bulbs of Crinum latifolium. Journal of Asian Natural Products Research, 23(11): 1023-1029.

Umar, H.I., Saliu, T.P., Josiah, S.S., Ajayi, A. and Danjuma, J.B. 2021. In silico studies of bioactive compounds from selected African plants with inhibitory activity against nitric oxide synthase and arginase implicated in asthma. Egyptian Journal of Medical Human Genetics, 22: 1-16.

Vuori, K.A. and Kanerva, M. 2018. Glutathione-S-Transferase (GST) activity assay for copepod samples. Journal of Biological Chemistry, 249: 7130-7139.

Walton, P.A., Brees, C., Lismont, C., Apanasets, O. and Fransen, M. 2017. The peroxisomal import receptor PEX5 functions as a stress sensor, retaining catalase in the cytosol in times of oxidative stress. Biochimica Et Biophysica Acta (BBA)-Molecular Cell Research, 1864(10): 1833-1843.

Xu, T., Liu, R., Zhu, H., Zhou, Y., Pei, T. and Yang, Z. 2022. The inhibition of LPS-induced oxidative stress and inflammatory responses is associated with the protective effect of (-)-Epigallocatechin-3-Gallate on bovine hepatocytes and murine liver. Antioxidants, 11(5): 914.

Yang, L., Palliyaguru, D.L. and Kensler, T.W. 2016. Frugal chemoprevention: targeting Nrf2 with foods rich in sulforaphane. In Seminars in oncology, 43(1): 146-153.

Zhou, Y., Hu, X., Zhong, S., Yu, W., Wang, J., Zhu, W., Yang, T., Zhao, G., Jiang, Y. and Li, Y. 2022. Effects of continuous LPS induction on oxidative stress and liver injury in weaned piglets. Veterinary Sciences, 10(1): 22-26.