استفاده از هسته انگور (Vitis vinifera) نانو کپسوله به‌منظور بهبود شاخص‌های شیمیایی، باکتریایی و افزایش زمان ماندگاری فیله قزل‌آلای رنگین‌کمان (Oncorhynchus mykiss)
محورهای موضوعی : میکروبیولوژی مواد غذاییفرانک محمودی اردبیلی 1 , مسعود هدایتی فرد 2
1 - دانش آموخته کارشناسی ارشد گروه شیلات، واحد قائم شهر، دانشگاه آزاد اسلامی، قائم شهر، ایران
2 - دانشیار گروه شیلات، دانشکده کشاورزی و منابع طبیعی، واحد قائم‌شهر، دانشگاه آزاد اسلامی، قائم‌شهر، ایران
کلید واژه: قزل آلای رنگین کمان, نانوانکپسوله, هسته انگور,
چکیده مقاله :
مقدمه: امروزه استفاده از نگهدارنده های طبیعی برای حفظ کیفیت مواد غذایی مورد توجه قرار گرفته است. در این پژوهش استفاده از نگهدارنده طبیعی عصاره هسته انگور در فرم نانوکپسوله برای افزایش عمر ماندگاری فیله ماهی قزل آلای رنگین کمان مورد بررسی قرار گرفت. مواد و روش ها: فیله های ماهی قزل آلای رنگین کمان پس از سر و دم زدن و تخلیه شکمی، حذف استخوان ها و شستشو، با عصاره هسته انگور با دو دز 2/0% و 4/0% در دو فرم آزاد (معمولی) و نانو انکپسوله بطور جداگانه تهیه و در دمای یخچال (4 درجه سانتی گراد) به مدت 15 روز نگهداری شد. شاخص های شیمیایی شامل بازهای نیتروژنی فرار (TVB-N mg/100)، عدد پراکسید (PV meqO2/Kfa) و تیوباربیتوریک اسید (TBA mgMDA/K) و جمعیت های باکتریایی شامل شمارش کلی باکتری های مزوفیل (TC) و باکتری های سرماگرا (TPC) مورد ارزیابی قرار گرفت. یافته‌ها: نتایج نشان داد که نمونه های حاوی انواع عصاره هسته انگور به هردو فرم نانو کپسوله و معمولی نسبت به نمونه شاهد دارای کیفیت بهتری بودند؛ بطوریکه زمان ماندگاری ماهی تازه از 6 روز در شاهد به بیش از 9 روز در نمونه های حاوی عصاره افزایش یافت (p<0.05). نتایح همچنین نشان داد فرآیند نانو انکپسول کردن عصاره هسته انگور بر روی جمعیت باکتری های سرماگرا (TPC)، تاثیری مشابه با فرم آزاد آن دارد؛ در حالیکه روی جمعیت باکتری های کل (TC) و نیز شاخص هایی همچون مجموعTVB-N ، اندیس PV و شاخص TBA اثر نگهدارندگی مستقل دارد (p<0.05). نتیجه‌گیری: با عنایت به نتایج بدست آمده می توان ادعا نمود که فیله ماهی قزل آلای رنگین کمان با به کارگیری عصاره هسته انگور تا 12 روز در یخچال قابلیت مصرف به عنوان ماهی تازه را دارد.
Introduction: Natural preservatives that have been employed for many years are employed to keep the quality of foods characteriestics. In this investigation the application of natural preservatives in the form of Nano-Encapsulated grape seed extracts have been studied to increase the shelf life of rainbow trout fillets. Matherials and Methods: Rainbow trout fillets were prepared after be heading, gutting, deboning and washing and prepared with grape seed extract with two doses of 2 and 4% of free (normal) and Nano-encapsulated (NEn) forms separately, and refrigerated at 4°C for 15 days. Chemical and microbiological characteristics consisted of total volatiles nitrogen, peroxide value and Thiobarbituric acid, total mesophyll count (TC) and psychrophilic count (TPC) were evaluated. Results: The results showed that all treated samples which contain samples in both free and NEn forms had better quality; therfore the shelf life increased considerably (p<0.05). The results also showed that the effect of grape seed NEn on the TPC bacteria is the same as the free form while has different and independent effects on the TC community and some other indices such as TVB-N, PV and TBA (p<0.05). Conclusion: According to the results, it can be claimed that rainbow trout fillet might be preserved for 12 days by the application of grape seed extract when kept in the refrigerator.
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