بررسی کارایی پلیمر قالب مولکولی در حذف بقایای آنتی بیوتیک اکسی تتراسایکلین در نمونه‌های سینتتیک و شیر
محورهای موضوعی :
علوم و صنایع غذایی
سعید جعفری
1
,
محمد دهقانی
2
,
الهام قرشی
3
,
نوید نصیری زاده
4
1 - دانش‌آموخته کارشناسی ارشد مهندسی پلیمر، باشگاه پژوهشگران جوان و نخبگان، واحد یزد، دانشگاه آزاد اسلامی، یزد، ایران
2 - دانشجو دکتری نساجی، باشگاه پژوهشگران جوان و نخبگان، واحد یزد، دانشگاه آزاد اسلامی، یزد، ایران
3 - دانشجوی کارشناسی ارشد مهندسی صنایع غذایی، دانشکده کشاورزی و منابع طبیعی، واحد یزد، دانشگاه آزاد اسلامی، یزد، ایران
4 - دانشیار، دانشکده مهندسی نساجی و پلیمر، واحد یزد، دانشگاه آزاد اسلامی، یزد، ایران
تاریخ دریافت : 1396/06/23
تاریخ پذیرش : 1397/01/24
تاریخ انتشار : 1397/04/01
کلید واژه:
شیر,
اکسی تتراسایکلین,
حذف,
پلیمر قالب مولکولی,
چکیده مقاله :
از نگرانی‌های رو به رشد چند دهه اخیر در زمینه آلودگی محیط‌زیست، ایجاد مقاومت دارویی در انسان به‌علت مصرف آنتی‌بیوتیک‌ها می‌باشد. هدف از انجام این پژوهش سنتز ذرات پلیمر قالب مولکولی جهت حذف آنتی‌بیوتیک اکسی‌تتراسایکلین از نمونه‌های آبی و بیولوژیکی (نمونه شیر) به روش غیرکووالانسی می‌باشد. تأثیر عوامل محیطی نظیر pH محلول (10-2)، زمان تماس (120-5 دقیقه) و مقدار ذرات پلیمر قالب مولکولی (1- 1/0گرم بر لیتر) در بهینه‌سازی شرایط حذف مورد بررسی قرار گرفت. ذرات پلیمری سنتز شده با استفاده از میکروسکوپ الکترونی روبشی و جذب و واجذب گاز نیتروژن شناسایی شدند. نتایج نشان داد که راندمان حذف در شرایط خنثی حداکثر بوده و با افزایش مقدار ذرات پلیمری راندمان حذف نیز افزایش می‌یابد. بهترین شرایط حذف اکسی‌تتراسایکلین در 5/6= pH، زمان 90 دقیقه و حداکثر ظرفیت جذب 280 میلی‌گرم برگرم تعیین شد. کارایی پلیمر سنتز شده در حذف اکسی‌تتراسایکلین از نمونه شیر مورد ارزیابی قرار گرفت. عملکرد ذرات پلیمری در حذف آنتی‌بیوتیک در نمونه‌های شیر تهیه شده از دامداری نشان داد که این نوع جاذب‌ها می‌توانند به کاهش مؤثر بقایای آلودگی‌های دارویی در نمونه‌های لبنی کمک کنند.
چکیده انگلیسی:
Among the growing concerns over recent decades in the field of environmental pollution, is the use of antibiotics increase bacterial resistance to antibiotics used in human body. The aim of this research was to synthesis of Molecularly Imprinted Polymer (MIP) particles for the Oxytetracycline (OTC) antibiotic removal from the aqueous and milk. The influence of different parameters such as pH (2-10), contact time (5-120 min) and MIP amounts (0.1 – 1.0 g/L) was studied on optimization of OTC removal. The synthesized MIP particles was characterized by scanning electron microscopy and absorption and desorption of nitrogen (BET). The results showed that the removal efficiency is higher in neutral pH and with increasing amounts of MIP particle, removal efficiency increases. The best condition for OTC removal was determined at pH, 6.5, 90 min and 280 mg/g as maximum absorption capacity. The MIP performance for OTC removal from milk was investigated too. The performance of MIP polymer particles in OTC removal in milk samples supplied from an animal husbandry in Yazd showed that these adsorbents can help to effectively reduce the residues of drug contaminants in dairy samples.
منابع و مأخذ:
· Algieri, C., Drioli, E., Guzzo, L. and Donato, L. (2014). Bio-Mimetic Sensors Based on Molecularly Imprinted Membranes. Sensors, 14(8): 13863-13912.
· Ali, W.H., Derrien, D., Alix, F., Pérollier, C., Lépine, O., Bayoudh, S. et al. (2010). Solidphase extraction using molecularly imprinted polymers for selective extraction of a mycotoxin in cereals. Journal of Chromatography A, 1217 (43): 6668-6673.
· Bautitz, I.R. and Nogueira, R.F. (2007). Degradation of tetracycline by photo-Fenton process-Solar irradiation and matrix effects. Journal of Photochemistry and Photobiology A: Chemistry, 187(1): 33-39.
· Chi, Y., Geng, W., Zhao, L., Yan, X., Yuan, Q., Li, N. et al., (2012). Comprehensive study of mesoporous carbon functionalized with carboxylate groups and magnetic nanoparticles as a promising adsorbent. Journal of colloids and interface Science, 369(1): 366-372.
· Dai, J.D., Zhou, Z.P., Zhao, C.Y., Wei, X., Dai, X.H., Gao, L. et al. (2014). Versatile Method To Obtain Homogeneous Imprinted Polymer Thin Film at Surface of Superparamagnetic Nanoparticles for Tetracycline Binding. Industrial & Engineering Chemistry Research, 53(17): 7157-7166.
· Davoodi, D., Hassanzadeh-Khayyat, M., Rezaei, M.A. and Mohajeri, S.A. (2014). Preparation, evaluation and application of diazinon imprinted polymers as the sorbent in molecularly imprinted solid-phase extraction and liquid chromatography up for analysis of ochratoxin A in ginger and LC-MS/MS confirmation. Food Control, 33(2): 337–343.
· Dobaradran, S., Nabizadeh, R., Mahvi, A.H., Mesdaghinia, A.R., Naddafi, K., Yunesian, M. et al., (2010). Survey on degradation rates of trichloroethylene in aqueous solutions by ultrasound. Journal of Environmental Health Science and Engineering, 7(4): 307-315.
· Elmolla, E.S. and Chaudhuri, M. (2011). The feasibility of using combined TiO2 photocatalysis-SBR process for antibiotic wastewater treatment. Desalination, 272(1): 218-224.
· Esfandiyari, T., Nasirizadeh, N., Dehghani, M. and Ehrampoosh, M.H. (2017). Graphene Oxide based Carbon Composite as Adsorbent for Hg Removal: Preparation, Characterization, Kinetics and Isotherms Studies. Chinese Journal of Chemical Engineering, 25 (9): 1170-1175.
· Gaballa, H. A., Geever, L. M., Killion, J. A. and Higginbotham, C. L. (2013). Synthesis and characterization of physically crosslinked N-vinylcaprolactam, acrylic acid, methacrylic acid, and N, N-dimethylacrylamide hydrogels. Journal of Polymer Science Part B: Polymer Physics, 51: 1555–1564.
· Huang, L., Sun, Y., Wang, W., Yue, Q. and Yang, T. (2011). Comparative study on characterization of activated carbons prepared by microwave and conventional heating methods and application in removal of oxytetracycline (OTC). Chemical Engineering Journal, 171(3):1446-1453
· Javanbakht, M., Pishro, K.A., Nasab, A.H. and Adergani, B.A. (2012). Extraction and purification of penicillin G from fermentation broth by water-compatible molecularly imprinted polymers. Materials Science and Engineering: C, 32(8): 2367– 2373.
· Jeong, J., Song, W., Cooper, W.J., Jung, J. and Greaves, J. (2010). Degradation of tetracycline antibiotics: mechanisms and kinetic studies for advanced oxidation/reduction processes. Chemosphere, 78 (5): 533-540.
· Khan, M.H., Bae, H. and Jung, J.Y. (2010). Tetracycline degradation by ozonation in the aqueous phase: Proposed degradation intermediates and pathway. Journal of hazardous materials, 181 (1): 659-665.
· Koenen-dierick K, Okerman L, Dezutter L, Degroodt JM, Van hoof J, Srebrnik S. (1995). A one plate microbiological screening test for antibiotic residue testing in kidney tissue and meat an alternative to the EEC four plate method. Food Additives and Containment, 12 (1): 77-82.
· Lu, C. and Su, F. (2007). Adsorption of natural organic matter by carbon nanotubes. Separation and Purification Technology, 58(1): 113–121.
· Lye, J. W. P., Saman, N., Sharuddin, S. S. N., Othman, N. S., Mohtar, S. S., Md Noor, A. M. et al. (2017). Removal Performance of Tetracycline and Oxytetracycline from Aqueous Solution via Natural Zeolites: An Equilibrium and Kinetic Study. Clean – Soil, Air, Water, 45 (10); 1-14.
· Mahvi, A., Maleki, A., Rezaee, R. and Safari, M. (2009). Reduction of humic substances in water by application of ultrasound waves and ultraviolet irradiation. Journal of Environmental Health Science and Engineering, 6(4): 233-240.
· Mahvi, A.H. (2009). Application of ultrasonic technology for water and wastewater treatment. Iranian Journal of Public Health, 38(2): 1-7.
· Mahvi, A.H. and Maleki, A. (2010). Photosonochemical degradation of phenol in water. Desalination Water Treatment, 20(1-3): 197-202.
· Maier, N.M., Buttinger, G., Welhartizki, S., Gavioli, E. and Lindner, W. (2004). Molecularly imprinted polymer-assisted sample clean-up of ochratoxin A from red wine: merits and limitations. Journal of Chromatography: B, 804: 103-111.
· Maleki, A., Mahvi, A.H., Mesdaghinia, A.R. and Naddafi K. (2007). Degradation and toxicity reduction of phenol by ultrasound waves. Bulletin of the Chemical Society of Ethiopia, 21(1): 33-38.
· Nasseh, N., Barikbin, B., Taghavi, L. and Nasseri, M.A. (2016). Antibiotics Pollution Damaging Effects on Environment and Review of Efficiency of Different Methods for removing them. Journal of Nurse and Physician within War, 10: 50-62. [in Persian]
· Ozkara, S., Andaç, M., Karakoç, V., Say, R., and Denizli A. (2011). Ion-Imprinted Phema Based Monolith for the Removal of Fe3+ Ions from Aqueous Solutions. Journal of Applied Polymer Science, 120: 1829-1836.
· Pan, B. and Xing, B. (2008). Adsorption mechanisms of organic chemicals on carbon nanotubes. Environmental Science and Technology, 42(24): 9005–9013.
· Rahimabadi, E., Asadpour, Y. and Sayeban, P. (2016). Survey on the tetracycline and oxytetracycline residues from milk collecting centers of Guilan by HPLC method, Iranian Veterinary Journal, 12(1): 188-124.
· Sahebnasagh, A., Karimi, G. and Mohajeri, S.A. (2014). Preparation and Evaluation of Histamine Imprinted Polymer as a Selective Sorbent in Molecularly Imprinted SolidPhase Extraction Coupled with High Performance Liquid Chromatography Analysis in Canned Fish. Food Analytical Methods, 7(1): 1-8.
· Shi, X., Wu, A., Zheng, S., Li, R. and Zhang, D. (2007). Molecularly imprinted polymer microspheres for solid-phase extraction of chloramphenicol residues in foods. Journal of Chromatography: B, 850: 24-30.
· Shirgholami, M.A., Mirjalili, M. and Nasirizadeh, N. (2017). Preparation of a MWCNT-Graphite Composite Based on Sol Gel Method for Dye Removal. Oriental Journal of Chemistry, 33(2): 1-10.
· Shojaei, S., Nasirizadeh, N., Entezam, M., Koosha, M. and Azimzadeh, M. (2016). An Electrochemical Nanosensor Based on Molecularly Imprinted Polymer (MIP) for Detection of Gallic Acid in Fruit Juices. Food Analytical Methods, 9: 2721–2731.
· Svenson, J. and Nicholls, I.A. (2001). On the thermal and chemical stability of molecularly imprinted polymers. Analytica Chimica Acta, 435: 19-24.
Zarangush, Z. and Mahdavi, S. (2017). Determination of Antibiotic Residues in Pasteurized and Raw Milk in Maragheh and Bonab Counties by Four Plate
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· Algieri, C., Drioli, E., Guzzo, L. and Donato, L. (2014). Bio-Mimetic Sensors Based on Molecularly Imprinted Membranes. Sensors, 14(8): 13863-13912.
· Ali, W.H., Derrien, D., Alix, F., Pérollier, C., Lépine, O., Bayoudh, S. et al. (2010). Solidphase extraction using molecularly imprinted polymers for selective extraction of a mycotoxin in cereals. Journal of Chromatography A, 1217 (43): 6668-6673.
· Bautitz, I.R. and Nogueira, R.F. (2007). Degradation of tetracycline by photo-Fenton process-Solar irradiation and matrix effects. Journal of Photochemistry and Photobiology A: Chemistry, 187(1): 33-39.
· Chi, Y., Geng, W., Zhao, L., Yan, X., Yuan, Q., Li, N. et al., (2012). Comprehensive study of mesoporous carbon functionalized with carboxylate groups and magnetic nanoparticles as a promising adsorbent. Journal of colloids and interface Science, 369(1): 366-372.
· Dai, J.D., Zhou, Z.P., Zhao, C.Y., Wei, X., Dai, X.H., Gao, L. et al. (2014). Versatile Method To Obtain Homogeneous Imprinted Polymer Thin Film at Surface of Superparamagnetic Nanoparticles for Tetracycline Binding. Industrial & Engineering Chemistry Research, 53(17): 7157-7166.
· Davoodi, D., Hassanzadeh-Khayyat, M., Rezaei, M.A. and Mohajeri, S.A. (2014). Preparation, evaluation and application of diazinon imprinted polymers as the sorbent in molecularly imprinted solid-phase extraction and liquid chromatography up for analysis of ochratoxin A in ginger and LC-MS/MS confirmation. Food Control, 33(2): 337–343.
· Dobaradran, S., Nabizadeh, R., Mahvi, A.H., Mesdaghinia, A.R., Naddafi, K., Yunesian, M. et al., (2010). Survey on degradation rates of trichloroethylene in aqueous solutions by ultrasound. Journal of Environmental Health Science and Engineering, 7(4): 307-315.
· Elmolla, E.S. and Chaudhuri, M. (2011). The feasibility of using combined TiO2 photocatalysis-SBR process for antibiotic wastewater treatment. Desalination, 272(1): 218-224.
· Esfandiyari, T., Nasirizadeh, N., Dehghani, M. and Ehrampoosh, M.H. (2017). Graphene Oxide based Carbon Composite as Adsorbent for Hg Removal: Preparation, Characterization, Kinetics and Isotherms Studies. Chinese Journal of Chemical Engineering, 25 (9): 1170-1175.
· Gaballa, H. A., Geever, L. M., Killion, J. A. and Higginbotham, C. L. (2013). Synthesis and characterization of physically crosslinked N-vinylcaprolactam, acrylic acid, methacrylic acid, and N, N-dimethylacrylamide hydrogels. Journal of Polymer Science Part B: Polymer Physics, 51: 1555–1564.
· Huang, L., Sun, Y., Wang, W., Yue, Q. and Yang, T. (2011). Comparative study on characterization of activated carbons prepared by microwave and conventional heating methods and application in removal of oxytetracycline (OTC). Chemical Engineering Journal, 171(3):1446-1453
· Javanbakht, M., Pishro, K.A., Nasab, A.H. and Adergani, B.A. (2012). Extraction and purification of penicillin G from fermentation broth by water-compatible molecularly imprinted polymers. Materials Science and Engineering: C, 32(8): 2367– 2373.
· Jeong, J., Song, W., Cooper, W.J., Jung, J. and Greaves, J. (2010). Degradation of tetracycline antibiotics: mechanisms and kinetic studies for advanced oxidation/reduction processes. Chemosphere, 78 (5): 533-540.
· Khan, M.H., Bae, H. and Jung, J.Y. (2010). Tetracycline degradation by ozonation in the aqueous phase: Proposed degradation intermediates and pathway. Journal of hazardous materials, 181 (1): 659-665.
· Koenen-dierick K, Okerman L, Dezutter L, Degroodt JM, Van hoof J, Srebrnik S. (1995). A one plate microbiological screening test for antibiotic residue testing in kidney tissue and meat an alternative to the EEC four plate method. Food Additives and Containment, 12 (1): 77-82.
· Lu, C. and Su, F. (2007). Adsorption of natural organic matter by carbon nanotubes. Separation and Purification Technology, 58(1): 113–121.
· Lye, J. W. P., Saman, N., Sharuddin, S. S. N., Othman, N. S., Mohtar, S. S., Md Noor, A. M. et al. (2017). Removal Performance of Tetracycline and Oxytetracycline from Aqueous Solution via Natural Zeolites: An Equilibrium and Kinetic Study. Clean – Soil, Air, Water, 45 (10); 1-14.
· Mahvi, A., Maleki, A., Rezaee, R. and Safari, M. (2009). Reduction of humic substances in water by application of ultrasound waves and ultraviolet irradiation. Journal of Environmental Health Science and Engineering, 6(4): 233-240.
· Mahvi, A.H. (2009). Application of ultrasonic technology for water and wastewater treatment. Iranian Journal of Public Health, 38(2): 1-7.
· Mahvi, A.H. and Maleki, A. (2010). Photosonochemical degradation of phenol in water. Desalination Water Treatment, 20(1-3): 197-202.
· Maier, N.M., Buttinger, G., Welhartizki, S., Gavioli, E. and Lindner, W. (2004). Molecularly imprinted polymer-assisted sample clean-up of ochratoxin A from red wine: merits and limitations. Journal of Chromatography: B, 804: 103-111.
· Maleki, A., Mahvi, A.H., Mesdaghinia, A.R. and Naddafi K. (2007). Degradation and toxicity reduction of phenol by ultrasound waves. Bulletin of the Chemical Society of Ethiopia, 21(1): 33-38.
· Nasseh, N., Barikbin, B., Taghavi, L. and Nasseri, M.A. (2016). Antibiotics Pollution Damaging Effects on Environment and Review of Efficiency of Different Methods for removing them. Journal of Nurse and Physician within War, 10: 50-62. [in Persian]
· Ozkara, S., Andaç, M., Karakoç, V., Say, R., and Denizli A. (2011). Ion-Imprinted Phema Based Monolith for the Removal of Fe3+ Ions from Aqueous Solutions. Journal of Applied Polymer Science, 120: 1829-1836.
· Pan, B. and Xing, B. (2008). Adsorption mechanisms of organic chemicals on carbon nanotubes. Environmental Science and Technology, 42(24): 9005–9013.
· Rahimabadi, E., Asadpour, Y. and Sayeban, P. (2016). Survey on the tetracycline and oxytetracycline residues from milk collecting centers of Guilan by HPLC method, Iranian Veterinary Journal, 12(1): 188-124.
· Sahebnasagh, A., Karimi, G. and Mohajeri, S.A. (2014). Preparation and Evaluation of Histamine Imprinted Polymer as a Selective Sorbent in Molecularly Imprinted SolidPhase Extraction Coupled with High Performance Liquid Chromatography Analysis in Canned Fish. Food Analytical Methods, 7(1): 1-8.
· Shi, X., Wu, A., Zheng, S., Li, R. and Zhang, D. (2007). Molecularly imprinted polymer microspheres for solid-phase extraction of chloramphenicol residues in foods. Journal of Chromatography: B, 850: 24-30.
· Shirgholami, M.A., Mirjalili, M. and Nasirizadeh, N. (2017). Preparation of a MWCNT-Graphite Composite Based on Sol Gel Method for Dye Removal. Oriental Journal of Chemistry, 33(2): 1-10.
· Shojaei, S., Nasirizadeh, N., Entezam, M., Koosha, M. and Azimzadeh, M. (2016). An Electrochemical Nanosensor Based on Molecularly Imprinted Polymer (MIP) for Detection of Gallic Acid in Fruit Juices. Food Analytical Methods, 9: 2721–2731.
· Svenson, J. and Nicholls, I.A. (2001). On the thermal and chemical stability of molecularly imprinted polymers. Analytica Chimica Acta, 435: 19-24.
Zarangush, Z. and Mahdavi, S. (2017). Determination of Antibiotic Residues in Pasteurized and Raw Milk in Maragheh and Bonab Counties by Four Plate
