تولید بیودیزل با استفاده از روش های بیوتکنولوژی و روغن میکروارگانیسمی
محورهای موضوعی : مدیریت محیط زیست
مرجان انشاییه
1
(باشگاه پژوهشگران جوان و نخبگان، واحد فلاورجان، دانشگاه آزاد اسلامی، اصفهان، ایران.)
آزاده عبدلی
2
(باشگاه پژوهشگران جوان و نخبگان، واحد فلاورجان، دانشگاه آزاد اسلامی، اصفهان، ایران.)
محبوبه مدنی
3
(گروه میکروبیولوژی، واحد فلاورجان، دانشگاه آزاد اسلامی ، اصفهان، ایران.)
کلید واژه: بیودیزل, مورتیرلا آلپینا, ترانس استریفیکاسیون, قارچ مولد چربی,
چکیده مقاله :
چکیده زمینه و هدف: مصرف زیاد از حد سوخت های فسیلی و تولید بیش از اندازه CO2 علاوه بر مشکلات مربوط به گرم شدن کره زمین، مسایل مربوط به آلودگی هوا را نیز در پی دارد. این موضوعات منجر به توجه بیش تر به تولید بیودیزل شده است. از جمله فواید تولید بیودیزل این است که به هنگام سوختن آن، انتشار خالص CO2 صورت نمی گیرد. بنابراین یافتن راهی زیستی برای تولید بیودیزل، نه تنها ارزش اقتصادی دارد بلکه از لحاظ زیست محیطی و تاثیر بر سلامتی انسان ها نیز ارزشمند است. هدف از این پژوهش به کارگیری قارچ مولد چربی جهت تولید تولید سوخت زیستی و همچنین بهینه سازی فرایند تولید بوده است. روش بررسی: در این پژوهش از قارچ مولد چربی مورتیرلا آلپینا با قابلیت تولید بالای چربی، جهت تولید روغن میکروبی استفاده شد. پس از آنالیز روغن تولید شده با کروماتوگرافی گازی- اسپکترومتری توده ای[1] (GC-MS)، امکان تبدیل آن به بیودیزل مورد ارزیابی قرار گرفت. بهینه سازی شرایط محیط نیز برای افزایش تولید لیپید صورت پذیرفت. شرایط بهینه به دست آمده در یک شبه فرمانتور آزمایشگاهی برای تولید لیپید مورد ارزیابی قرار گرفت. یافته‌ها: سویه قارچی مورد نظر دارای بیش ترین میزان تولید لیپید معادل g/L9/10 بود. این سویه پس از ترانس استریفیکاسیون دارای بازده تولید بیودیزل معادل 71% بوده و بیش ترین اسید چرب موجود در آن اولئیک اسید به میزان 38% به دست آمد. بحث و نتیجه‌گیری: ترکیب روغن تولید شده در این قارچ مشابهت زیادی با روغن های گیاهی داشته و در این زمینه برای تولید سوخت زیستی با لیپید های گیاهی قابل رقابت می باشد که از نظر اقتصادی بسیار ارزشمند است. [1]-Gas chromatography- Mass Spectrometry
Background and Objective: Excessive consumption of fossil fuels and excessive production of CO2, in addition to problems related to the golabal warming cause air pollution. This has attracted more attention to biodiesel production. Among the benefits of biodiesel production is that when it burns no net CO2 is emitted. Therefore, finding a biological way to produce biodiesel has not only economical value but also it is valuable in terms of environmental aspects and impact on human health. The goal of this study was application of oleaginous yeast for biodiesel production and optimization of production process. Method: In this study, the oleaginous fungus Mortierella alpina with high capacity of lipid production was used for microbial oil production. After analyzing the produced oil by gas chromatography-mass spectrometry (GC-MS), its potential for biodiesel production was evaluated. Also optimization of medium condition for increasing lipid production was done. The optimized condition was used in a semi laboratory (pilot-scale) fermentor I order to evaluate lipid production. Findings: The evaluated fungal strain had the highest lipid production of 10.9g/L. This strain had biodiesel yield of 71% and the highest fatty acid was oleic acid with amount of 38%. Conclusion: The lipid composition in this fungus has a high similarity with plants oil and can compete with plants lipid for biodiesel production and is valuable from economical point of view.
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