بررسی اثرات ضد انعقادی و ضدکلسترولی و آنتی بیوتیکی مواد موثره گانودرما لوسیدوم در شرایط برون تنی و درون تنی
محورهای موضوعی : زیست شناسی سلولی تکوینی گیاهی و جانوری ، تکوین و تمایز ، زیست شناسی میکروارگانیسم
مهیار شاکری راد
1
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محدثه لاری پور
2
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زهرا کشتمند
3
1 - گروه بیوتکنولوژی، واحد تهران شمال، دانشگاه آزاد اسلامی، تهران، ایران
2 - گروه میکروب شناسی، واحد تهران شمال، دانشگاه آزاد اسلامی، تهران، ایران
3 - گروه زیست شناسی، واحد تهران مرکزی، دانشگاه آزاد اسلامی، تهران، ایران
کلید واژه: ضد انعقاد, آنتی کلسترولی, پلی ساکارید, گانودریک اسید, گانودرما لوسیدوم,
چکیده مقاله :
بررسی اثرات سلامت بخش مواد موثره ی گانودرما در شرایط برون تنی و درون تنی هدف این مطالعه است. بانک کشت قارچ تهیه شد. استخراج و خالص سازی گانودریک اسید و پلی ساکارید از قارچ گانودرما لوسیدوم با روش کروماتوگرافی انجام شد. آنتی بیوگرام، اثرات پری بیوتیکی، ضد کلسترولی و ضد انعقادی پلی ساکارید و گانودریک اسید در اینویترو و اینویوو انجام شد. MIC و MBC پلی ساکارید برای اشریشیاکلای برابر با غلظت 40% بود و برای استافیلوکوکوس اورئوس 20% بود. MIC و MBC گانودریک اسید برای اشریشیاکلای و استافیلوکوکوس اورئوس برابر با غلظت20% بود. درصد وزن با اضافه شدن رژیم دریافت کننده چربی به صورت معنی داری افزایش یافت. با اضافه شدن پلی ساکارید قارچی به رژیم های غذایی میانگین وزن موش هاکاهش یافت (05/0(P<. کاهش وزن موش ها در گروه های دریافت کننده پلی ساکارید + گانودریک اسید بیشتر بود(05/0(P<. در مقایسه با گروه دیابتی، سطح سرمی کلسترل تام، تری¬گلیسرید و LDL در گروه دریافت کننده پلی ساکارید و گانودریک اسید کاهش معنی داری یافت و میزان HDL افزایش یافت. تغییرات کلسترول تحت تیمار معنی دار نبود(05/0(P≥. زمان انعقاد فقط در گروه های دریافت کننده پلی ساکارید + گانودریک اسید (رژیم چرب) و پلی ساکارید + گانودریک اسید (رژیم معمولی) نسبت به گروه شاهد(چرب) افزایش معنی دار داشت(05/0(P< و تاثیر آنتی کلسترولی پلی ساکارید استخراجی بیشتر از گانودریک اسید بود. مصرف مکمل غذایی گانودرما، اثرات بیولوژیک فراوان دارد.
The purpose of this study is to investigate the health effects of Ganoderma active ingredients in in vitro and in vitro conditions. A mushroom culture bank was prepared. Extraction and purification of ganodriic acid and polysaccharide from Ganoderma lusidum were done by the chromatography method. Antibiogram, prebiotic, anticholesterol and anticoagulant effects of polysaccharide and ganoderic acid were performed in vitro and in vivo. MIC and MBC of polysaccharide for Escherichia coli was 40% and for Staphylococcus aureus it was 20%. MIC and MBC of ganoderic acid for Escherichia coli and Staphylococcus aureus was equal to 20% concentration. Weight percentage increased significantly with the addition of a fat-receiving diet. With the addition of mushroom polysaccharides to the diets, the average weight of mice decreased (P<0.05). The weight loss of mice in the groups receiving polysaccharide + ganoderic acid was greater (P<0.05). Compared to the diabetic group, serum levels of total cholesterol, triglycerides, and LDL in the group receiving polysaccharide and ganoderic acid decreased significantly, and HDL levels increased. Cholesterol changes under the treatment were not significant (P≥0.05). Coagulation time increased only in the groups receiving polysaccharide + ganoderic acid (fat diet) and polysaccharide + ganoderic acid (normal diet) compared to the control group (fat). It was significant (P<0.05) and the anticholesterol effect of extracted polysaccharide was more than ganoderic acid. Ganoderma dietary supplement has many biological effects.
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