بررسی ترمیم نقص استخوان بااستفاده ازغشاءژلاتین-کیتوسان وسلول‌های بنیادی مشتق ازچربی درموش صحرایی(ارزیابی هیستومورفومتری وایمونوهیستولوژیک)
محورهای موضوعی : زیست شناسی سلولی تکوینی گیاهی و جانوری ، تکوین و تمایز ، زیست شناسی میکروارگانیسم
کلید واژه: موش صحرایی, سلول‌هایADSC, غشاءژلاتین-کیتوسان, پیوندسلول, ترمیم استخوان,
چکیده مقاله :
(ADSCs)سلولهایچنداستعدادیهستندکهقابلیتتمایزبهسلول هایاستئوژنیکرادارند.ازداربست هایکیتوسانوژلاتینکهساختاریزیستتخریبپذیروزیستسازگاردارندجهتترمیمبافت هااستفادهمی شوند.دراینبررسی60سرموشصحرائیبهطورتصادفیدرپنجگروهقرارگرفتند.1-شاهد:هیچگونهدرمانیدریافتنکردند2-شم:محیطکشتدرمحلنقصتزریقشد3-غشاء:ازژلاتین-کیتوساندرمحلنقصاستخوانیاستفادهشد.4-سلول:پیوندسلول هایADSCدرمحلنقصانجامشد.5-سلول–غشاء:سلولبههمراهغشاءژلاتین-کیتوساندرمحلنقصپیوندگردید.ارزیابی هایایمونوهیسیتوشیمیوهیستومورفومتریدرگروه هایمختلفموردبررسیقرارگرفت.میانگینمساحتترابکولایاستخوانیدرگروه هایغشاء،سلولوسلول-غشاءنسبتبهگروهشاهدافزایشمعنی داریرانشانداد.همچنینمیانگینتعداداستئوسیت هادرناحیهنقصاستخوانیدرگروه هایسلولوسلول-غشاءنسبتبهگروهشاهدکاهشمعنی داریداشتندگرچهدرگروهغشاءکاهشبصورتغیرمعنی دارمشاهدهگردید.بهنظرمی رسدکهاستفادهازداربستغشاءژلاتین-کیتوسانهمراهباپیوندسلول هایADSCجهتترمیمنقصاستخوانیموثرباشد.
Adipose - derived stem cells are multipotent cells that capable to differentiate into osteogenic cells, the scaffold of chitosan and gelatin as biodegradable and compatible used for tissues repair. The control group that received no treatment after the bone defect, 2 sham : that the defect in the bone defect was injected medium, 3- (gelatin - chitosan) that the membrane gelatin - chitosan was used into the bone defect. 4. ADSC: that ADSC cell transplanted into the defect. 5 : (cell - the cell membrane of gelatin – chitosan) was used into the bone defect. Histomorphometric evaluations in the different groups studied. The mean area of trabeculae in groups of membranes, cells and cell - membrane significantly increased when compared to the control group. Also the mean number of osteocytes and cells in the bone defect in groups of cells and cell - membrane significantly decreased when compared to the control group. Although in the chitosan - gelatin group, mean number of osteocytes decreased but were not significant. It seems that use of scaffold of chitosan - gelatin membrane and ADSC cell transplantation to be effective in repair of bone defect.
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