بررسی اثر نور آبی و قرمز بر بیان ژن CRY1و HY5در دانه رست گیاه کلزا (Brassica napus L.)
محورهای موضوعی : زیست شناسی سلولی تکوینی گیاهی و جانوری ، تکوین و تمایز ، زیست شناسی میکروارگانیسم
کلید واژه: کلزا, نور آبی, نور قرمز, ژن کریپتوکروم1, ژن HY5,
چکیده مقاله :
در چند دهه اخیر، تحقیقات متعددی برای ارزیابی بیان ژن‌های موثر از نور آبی و قرمز، در گیاهان،انجام گرفته است. هدف از این پژوهش، بررسی اثرات نور آبی و قرمز بر میزان بیان ژن کریپتوکروم 1 و HY5 در دانه‌رست‌های گیاه کلزا،بود. دانه‌رست‌های کلزا در شرایط یکنواخت محیطی، و سپس 5 روز تحت تیمار نور آبی (به مدت 2 ساعت، 4 ساعت، 8 ساعت) و نور قرمز (به مدت 2 ساعت ،4 ساعت ،8 ساعت) قرار گرفتند و تفاوت طول هیپوکوتیل در تیمارهای مختلف، مشاهده شد و میزان بیان ژن کریپتوکروم 1 توسط کنیک RT-PCR (Semiquantitative Reverse Transcription-Polymerase Chain Reaction) بررسی شد، همچنین میزان بیان ژن HY5 به موازات بیان ژن CRY1، توسط تکنیک (Quantitative real-time) qRT-PCR مورد بررسی قرار گرفت. نتایج بررسی های اثر تیمار نور آبی بر گیاهان شاهد و تحت تیمار، نشان داد که نور آبی قادر به تنظیم بیان ژن کریپتوکروم1، و در نتیجه کنترل ریخت زایی نوری(فتومورفوژنز) در گیاه (Brassica napus L.) شد به طوری که در 8 ساعت تیمار با نور آبی، بیشترین میزان بیان ژن کریپتوکروم 1، مشاهده شد، البته سطح بیان ژن HY5نیز در تیمار نور آبی به بالاترین حد خود رسید که نتیجه آن بیشترین میزان منع کردن از بلند شدن هیپوکوتیل در این گروه خاص بود.
In recent decades, several researches have been done on plants in order to evaluate the expression of genes influenced by blue and red light. The aim of present study is analyzing the effects of blue and red light on the expression of CRY1 and HY5 genes in rape seedlings. These seedlings were placed in steady environmental conditions and then in blue light treatment (2, 4 or 8 hr) and in red light treatment (2, 4 or 8 hr) for 5 days, and the variation of hypocotyl length in various treatments was scrutinized and the expression level of CRY1 gene was analyzed by Semi-quantitative Reverse Transcription-Polymerase Chain Reaction (RT-PCR) technique. Also, the amount of HY5 gene expression along with CRY1 gene expression was examined by Quantitative Real-time-Polymerase Chain Reaction (qRT-PCR) technique. Results of studying the effects of blue light treatment on control and treated plants showed that the blue light is capable to adjust expressing CRY1 gene and thus to control photomorphogenesis in rape (Brassica napus L.) plants, so that in an 8-hour treatment with blue light, we observed the highest level of CRY1 expression. Of course, the expression level of HY5 gene reached to its highest point, which resulted in maximum inhibition of hypocotyl elongation in this special group.
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