بررسی بیوانفورماتیکی FLORICAULA (FLO) در گیاه زینتی بنفشه آفریقایی (Saintpaulia ionantha H.Wendl.)
محورهای موضوعی :
زیست شناسی سلولی تکوینی گیاهی و جانوری ، تکوین و تمایز ، زیست شناسی میکروارگانیسم
مینا کاظمیان
1
,
الهام محجل کاظمی
2
,
مریم کلاهی
3
,
ولی اله قاسمی
4
1 - گروه گیاهی، دانشکده علوم طبیعی، دانشگاه تبریز، تبریز، ایران.
2 - گروه گیاهی، دانشکده علوم طبیعی، دانشگاه تبریز، تبریز، ایران.
3 - گروه زیست‌شناسی، دانشکده علوم، دانشگاه شهید چمران اهواز، اهواز، ایران
4 - پژوهشکده ژنتیک و زیست‌فناوری طبرستان، ساری، مازندران، ایران.
تاریخ دریافت : 1401/08/22
تاریخ پذیرش : 1401/10/11
تاریخ انتشار : 1402/09/01
کلید واژه:
توالی یابی,
فیلوژنی,
خانواده FLO,
صفحات بتا,
چکیده مقاله :
گذر مرحله رویشی به زایشی در گیاه زینتی بنفشه آفریقایی با نام علمی Saintpaulia ionantha H.Wendlبا کنترل فاکتورهایی از جمله FLORICAULA (FLO) صورت می‌گیرد. فرآیند تعیین سرنوشت مریستم گل و الگوی شکل‌گیری آن توسط ژن FLO و القای ژن‌های همئوتیک مشخص می‌شود. در این پژوهش تعیین توالی پروتئین FLO، ویژگی‌های پروتئینی، ساختار دوم، موقعیت درون-سلولی و درخت فیلوژنتیکی توسط ابزارهای بیوانفورماتیک برای نخستین‌بار در بنفشه آفریقایی مورد ارزیابی قرارگرفته است. نتایج نشان داد که موقعیت درون سلولی این پروتئین در هسته پیش بینی شده است. این پروتئین (FLO) به خانواده FLO/LFY تعلق دارد. ساختار ثانویه پروتئین 53% آلفا هلیکس و 4% صفحات بتا تشکیل شده است. بررسی درخت فیلوژنی، گونه‌های مربوط به یک خانواده در یک شاخه جای گرفتند. همچنین هم‌ترازی توالی پروتئین نشان داده است که در گیاهان هم‌خانواده توالی به‌شدت حفاظت شده است. شناسایی ویژگی‌های پروتئین FLO می‌تواند به عنوان فاکتور اصلی در برنامه‌ریزی عملکرد بافت و به دنبال آن کنترل عملکرد این پروتئین حائز اهمیت باشد.
چکیده انگلیسی:
The transition from vegetative to reproductive stage in Saintpaulia ionantha H.Wendl is controlled by factors such as FLORICAULA (FLO). The process of determining the fate of the flower meristem and its formation pattern is determined by the FLO and the induction of homeotic regulators. In this research, FLO sequencing, protein characteristics, secondary structure, intracellular position and phylogenetic tree have been evaluated by bioinformatics tools for the first time in African violet. The results showed that the intracellular location of this protein is predicted in the nucleus. This protein (FLO) belongs to the FLO/LFY family. The secondary structure of the protein consists of 53% alpha helix and 4% beta sheets. Examining the phylogeny tree, species related to one family were placed in one branch. Also, protein sequence alignment has shown that the sequence is highly conserved in plants of the same family. Identifying the characteristics of FLO protein can be important as the main factor in planning the function of the tissue and then controlling the function of this protein.
منابع و مأخذ:
Ghalecahi B, Aslanpour M, Shoor M, Sharifi A, Kharazi M. Effect of light variables treatments on growth and flowering of saintpaulia (Saintpaulia ionantha). ITJEMAST, 2018; 9(6): 597-609.
Mercuri A, De Benedetti L, Burchi G. Agrobacterium-mediated transformation of African violet. PCTOC, 2000; 60: 39.
Lowenstein DM, Matteson KC, Minor ES. Evaluating the dependence of urban pollinators on ornamental, non-native, and ‘weedy’ floral resources. Urban Ecosystem, 2019; 22(2): 293-302,.
Weber A, Clark JL, Moller M. A new formal classification of Gesneriaceae. Selbyana, 2013; 31(2): 68-94.
Haston E, De Craene LPR. Inflorescence and floral development in Streptocarpus and Saintpaulia (Gesneriaceae) with particular reference to the impact of bracteole suppression. Plant Sys Evol, 2007; 265: 13–25.
Teixeira da Silva J.A, Dewir Y, Wicaksono A, Kher M, Kim H, Hosokawa M, Zeng S. Morphogenesis and developmental biology of African Violet (Saintpaulia ionantha WENDL.). J Plant Dev, 23: 15-25, 2016.
Roberts W.R, Roalson E.H. Comparative transcriptome analyses of flower development in four species of Achimenes (Gesneriaceae). BMC Genomics, 2017; 18:1-26.
Yang S.J, Ohno Sh, Deguchi A, Sato M, Goto M, Doi M. The histological study in sympetalous corolla development of pinwheel-type flowers of Saintpaulia. Scientiae Horticulturae. 2017; 223: 10-18.
Tang M, Tao YB, Fu Q, Song Y, Niu L, Xu ZF. An ortholog of LEAFY in Jatropha curcas regulates flowering time and floral organ development. Science Reports, 2016; 6:37306.
Ahmad S, Li Y, Yang Y, Zhou Y, Zhao K, Zhang Q. Plant Cell Tiss Organ Cult, 2019; 136: 523.
Moyroud E, Minguet E.G, Ott F, Yant L, Posé D, Monniaux M, Blanchet S, Bastien O, Thévenon E, Weigel D, Schmid M, Parcy F. Prediction of regulatory interactions from genome sequences using
a biophysical model for the Arabidopsis LEAFY transcription factor. Plant Cell, 2011; 23: 1293–1306.
Winter CM, Austin R.S, Blanvillain-Baufume S, Reback MA, Monniaux, M, Wu MF. LEAFY target genes reveal floral regulatory logic cis motifs, and a link to biotic stimulus response. Development Cell, 2011; 20: 430–443.
Coen E, Romero J, Doyle S, Elliott R, murphy G, Carpenter R. Floricaula: A Homeotic Gene Required for Flower Development in Antirrhinum majus. Cell, 1990; 63:1311-1322.
McKim S, Hay A. Patterning and evolution of floral structures – marking time. Curr. Opin. Genet. Dev. 2010; 20: 448-453.
Liu B, Wang L, Wang Sh, Li W, Liu D, Guo X, Qu B. Transcriptomic analysis of bagging-treated ‘Pingguo’ pear shows that MYB4-like1, MYB4-like2, MYB1R1 and WDR involved in anthocyanin biosynthesis are up-regulated in fruit peels in response to light. Scietia Horticulturae. 2019; 244: 428-434.
Kalpana K, Manjuvani S, Shoba K. 2018. In Silico Comparative Modeling of Maturase K Protein in Cymbopogon martinii 2018; J Bioinformatics, 5(3): 30-36.
Faghani E, Kolahi M, Kazemian M, Goldson-Barnaby A, Razzaghi MH. Effect of irrigation regimes on starch biosynthesis pathway, cotton (Gossypium hirsutum) yield and in silico analysis of ADP-glucose-pyrophosphorylase. J. Environ. Sci. Technol, 2022; 19: 10809–10830.
Kazemian M, Mohajel Kazemi E, Kolahi M, Ghasemi Omran V. Floral ontogeny and molecular evaluation of anthocyanin biosynthesis pathway in pinwheel phenotype of Saintpaulia inontha periclinal chimera. Scientia Horticulturae, 2020; 263: 109142.
Wang CN, Möller M, Cronk QC. Altered expression of GFLO, the Gesneriaceae homologue of FLORICAULA/LEAFY, is associated with the transition to bulbil formation in Titanotrichum oldhamii. Dev Genes Evolution, 214(3):122-7, 2004.
Zhang Ch, Zhang H, Zhan Z, Liang Y. Molecular cloning, expression analysis and subcellular localization of LEAFY in carrot (Daucus carota). Mol. Breed, 2016;36:1-9.
Li J, Fan SL, Song MZ, Pang CY, Wei HL, Li W, Ma JH, Wei JH, Jing JG, Yu SX. Cloning and characterization of a FLO/LFY ortholog in Gossypium hirsutum Plant Cell Rep., 2013; 32(11):1675-86.
An L, Lei H, Shen X, Li T. Plant Mol. Biol Report, 2012; 30:1488.
Jang S. Functional Characterization of PhapLEAFY, a FLORICAULA/ LEAFY Ortholog in Phalaenopsis aphrodite. Plant Cell Physiology, 2016; 56(11): 2234-2247.
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Ghalecahi B, Aslanpour M, Shoor M, Sharifi A, Kharazi M. Effect of light variables treatments on growth and flowering of saintpaulia (Saintpaulia ionantha). ITJEMAST, 2018; 9(6): 597-609.
Mercuri A, De Benedetti L, Burchi G. Agrobacterium-mediated transformation of African violet. PCTOC, 2000; 60: 39.
Lowenstein DM, Matteson KC, Minor ES. Evaluating the dependence of urban pollinators on ornamental, non-native, and ‘weedy’ floral resources. Urban Ecosystem, 2019; 22(2): 293-302,.
Weber A, Clark JL, Moller M. A new formal classification of Gesneriaceae. Selbyana, 2013; 31(2): 68-94.
Haston E, De Craene LPR. Inflorescence and floral development in Streptocarpus and Saintpaulia (Gesneriaceae) with particular reference to the impact of bracteole suppression. Plant Sys Evol, 2007; 265: 13–25.
Teixeira da Silva J.A, Dewir Y, Wicaksono A, Kher M, Kim H, Hosokawa M, Zeng S. Morphogenesis and developmental biology of African Violet (Saintpaulia ionantha WENDL.). J Plant Dev, 23: 15-25, 2016.
Roberts W.R, Roalson E.H. Comparative transcriptome analyses of flower development in four species of Achimenes (Gesneriaceae). BMC Genomics, 2017; 18:1-26.
Yang S.J, Ohno Sh, Deguchi A, Sato M, Goto M, Doi M. The histological study in sympetalous corolla development of pinwheel-type flowers of Saintpaulia. Scientiae Horticulturae. 2017; 223: 10-18.
Tang M, Tao YB, Fu Q, Song Y, Niu L, Xu ZF. An ortholog of LEAFY in Jatropha curcas regulates flowering time and floral organ development. Science Reports, 2016; 6:37306.
Ahmad S, Li Y, Yang Y, Zhou Y, Zhao K, Zhang Q. Plant Cell Tiss Organ Cult, 2019; 136: 523.
Moyroud E, Minguet E.G, Ott F, Yant L, Posé D, Monniaux M, Blanchet S, Bastien O, Thévenon E, Weigel D, Schmid M, Parcy F. Prediction of regulatory interactions from genome sequences using
a biophysical model for the Arabidopsis LEAFY transcription factor. Plant Cell, 2011; 23: 1293–1306.
Winter CM, Austin R.S, Blanvillain-Baufume S, Reback MA, Monniaux, M, Wu MF. LEAFY target genes reveal floral regulatory logic cis motifs, and a link to biotic stimulus response. Development Cell, 2011; 20: 430–443.
Coen E, Romero J, Doyle S, Elliott R, murphy G, Carpenter R. Floricaula: A Homeotic Gene Required for Flower Development in Antirrhinum majus. Cell, 1990; 63:1311-1322.
McKim S, Hay A. Patterning and evolution of floral structures – marking time. Curr. Opin. Genet. Dev. 2010; 20: 448-453.
Liu B, Wang L, Wang Sh, Li W, Liu D, Guo X, Qu B. Transcriptomic analysis of bagging-treated ‘Pingguo’ pear shows that MYB4-like1, MYB4-like2, MYB1R1 and WDR involved in anthocyanin biosynthesis are up-regulated in fruit peels in response to light. Scietia Horticulturae. 2019; 244: 428-434.
Kalpana K, Manjuvani S, Shoba K. 2018. In Silico Comparative Modeling of Maturase K Protein in Cymbopogon martinii 2018; J Bioinformatics, 5(3): 30-36.
Faghani E, Kolahi M, Kazemian M, Goldson-Barnaby A, Razzaghi MH. Effect of irrigation regimes on starch biosynthesis pathway, cotton (Gossypium hirsutum) yield and in silico analysis of ADP-glucose-pyrophosphorylase. J. Environ. Sci. Technol, 2022; 19: 10809–10830.
Kazemian M, Mohajel Kazemi E, Kolahi M, Ghasemi Omran V. Floral ontogeny and molecular evaluation of anthocyanin biosynthesis pathway in pinwheel phenotype of Saintpaulia inontha periclinal chimera. Scientia Horticulturae, 2020; 263: 109142.
Wang CN, Möller M, Cronk QC. Altered expression of GFLO, the Gesneriaceae homologue of FLORICAULA/LEAFY, is associated with the transition to bulbil formation in Titanotrichum oldhamii. Dev Genes Evolution, 214(3):122-7, 2004.
Zhang Ch, Zhang H, Zhan Z, Liang Y. Molecular cloning, expression analysis and subcellular localization of LEAFY in carrot (Daucus carota). Mol. Breed, 2016;36:1-9.
Li J, Fan SL, Song MZ, Pang CY, Wei HL, Li W, Ma JH, Wei JH, Jing JG, Yu SX. Cloning and characterization of a FLO/LFY ortholog in Gossypium hirsutum Plant Cell Rep., 2013; 32(11):1675-86.
An L, Lei H, Shen X, Li T. Plant Mol. Biol Report, 2012; 30:1488.
Jang S. Functional Characterization of PhapLEAFY, a FLORICAULA/ LEAFY Ortholog in Phalaenopsis aphrodite. Plant Cell Physiology, 2016; 56(11): 2234-2247.
