مطالعه مورفولوژی و مورفومتری بیضه گربه به دنبال تجویز مقادیر مختلف بوسولفان

محورهای موضوعی : پاتوبیولوژی مقایسه ای

مهدی عصمت‌پرست، پرویز تاجیک*، حمید قاسم‌زاده‌نوا . ¹

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تاریخ دریافت : 1397/01/24 تاریخ پذیرش : 1397/01/24 تاریخ انتشار : 1397/01/01

کلید واژه: بوسولفان, اسپرماتوژنز, بیضه, گربه,

چکیده مقاله :

به منظور موفقیت در پیوند سلول‌های اسپرماتوگونی نیاز است تا در اولین گام مدل مناسبی برای تولید حیوانات آزواسپرمیک تعریف گردد. روش‌های متعددی جهت تولید حیوانات آزواسپرمیک موجود است که این موارد شامل استفاده از بوسولفان به صورت سیستمیک، رادیوتراپی، ایسکمی سرمایی و افزایش دمای بیضه می‌باشند. هدف از انجام این طرح ابداع روشی جهت تولید گربه‌های آزواسپرمیک به منظور آماده‌سازی آنها برای دریافت پیوند سلول‌های بنیادی اسپرماتوگونی با تزریق بوسولفان می‌باشد. در این مطالعه از 15 راس گربه نر مو کوتاه خانگی 3 الی 5 ماهه استفاده شد. پس از اولین تزریق در هفته‌ی 5، 9، 13 و 17 دوزهای صفر، 4 و 10 میلی‌گرم/کیلوگرم وزن بدن، بوسولفان به صورت داخل وریدی به ترتیب به گربه‌های گروه‌های شاهد و تیمار تزریق شد. بیضه‌ها 2 ماه پس از پایان تزریقات بوسیله جراحی خارج شد و مورد ارزیابی قرار گرفت. همچنین میانگین قطر مجرای داخل لوله اسپرم ساز و ضخامت اپیتلیوم زاینده براساس میکرومتر محاسبه شد. سلول‌های سرتولی و سلول‌های لیدیگ نیز مورد ارزیابی قرار گرفت. در بررسی بافتی در گروه‌های تحت درمان با دوزهای 4 و 10 میلی‌گرم/کیلوگرم وزن بدن بوسولفان، درجات متفاوتی از تخریب لوله‌های اسپرم ساز، کاهش تعداد لوله‌های اسپرم ساز و ضخامت اپیتلیوم زاینده مشاهده شد. بوسولفان از طریق تأثیر بر سلول‌های زاینده و سوماتیک موجود در بیضه می‌تواند در تخریب اسپرماتوژنز و القاء آزواسپرمی در بیضه نقش داشته باشد.

چکیده انگلیسی:

In order to successfully transplant spermatogonial cells need to be defined a suitable model for producing azoospermia animals. Several methods are available for made azoospermic animals, include systemic injection of busulfan, radiation, cold ischemia and increased testicular temperature. The aim of the study is development of a method for producing azoospermic cats in order to prepare them for spermatogonial stem cells transplantation. In this study, 15 domestic short hair cats at the age of 3 to 5 month were divided in 3 groups. After first injection, at 5, 9, 13 and 17 weeks, doses of zero, four and ten mg/kg of busulfan intravenously were injected to the control and treatment groups, respectively. Two months after the injections testicles surgically removed and evaluated. The mean diameter of the seminiferous tubules and germinal epithelium thickness was calculated. Sertoli and leydig cells were also evaluated. Histological evaluation of treating groups with 4 and 10 mg/kg busulfan shows different degrees of degradation of seminiferous tubules. The thickness of germinal epithelium significantly decreased. Busulfan through its effect on germ cells and somatic cells can disrupt spermatogenesis and induced azoospermia.

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