The Induction of Tolerance to Salinity Stress of Commercial Sugarcane Cultivar CP72-1312 by in Tissue Culture Condition
Subject Areas : Journal of Crop Nutrition ScienceZahra Khodarahmpor 1 , Seyed Ehsan Emam 2
1 - Department of Agronomy and Plant Breeding, Shoushtar Branch, Islamic Azad University, Shoushtar, Iran.|Tropical and Subtropical Crops Research Center, Shoushtar Branch, Islamic Azad University, Shoushtar, Iran.
2 - Department of Agricultural Research, Karun Agro Industry Company, Shoushtar, Iran.
Keywords: Rooting, regeneration, Callus induction, <i>NaCl, Strain</i>,
Abstract :
BACKGROUND: In order to investigate the induction of salinity tolerance in CP72-1312 commercial cultivar in tissue culture condition, an experiment was carried out in the tissue culture laboratory of Karoun Agro-Industry Company in 2017 year. OBJECTIVES: At this study we evaluated tolerance of CP73-1312 sugarcane commercial cultivar to salt stress in tissue culture. METHODS and RESULT: Six callus induction treatments in Murashig and Skoog based medium were studied in a CRD with 4 replications. MS based medium supplemented with 2,4-D in 3 mg.l-1 was the best treatment for callus induction (93.3%). The effect of different levels of salinity (0.2, 0.4, 0.6, 0.8 and 1 percent) on callus tolerance was studied in a CRD with 4 replications. In the eighth weeks, the callus value reduction in compare to control was obtained 23, 51, 66, 75 and 81 percent, respectively. Five regeneration treatments in modified MS were performed as factorial experiment in a CRD with 4 replications. The highest regeneration rate was 1 mg.l-1 BAP and 0.02 mg.l-1 KIN. This treatment was used for indirect regeneration under salinity stress condition. The mean comparisons showed that the highest regeneration rate was observed in the control treatment and the lowest regeneration was observed in salinity treatment 0.8 and 1 percent. Five root treatments in half-strength MS medium were studied in a CRD with 4 replications with IBA. The highest rooting rates were assigned to 0.01 and 0.02 mg.l-1 IBA treatments. The effect of the best level of IBA (0.01 mg.l-1) on salinity stress was evaluated on rooting. CONCLUSION: According to the results, with increasing salinity stress, rooting rate decreased.
Al-Maskri, A., L. Al-Kharusi. and H. Al-Miqbali. 2010. Effects of salinity stress on growth of lettuce (Lactuca sativa) under closed-recycle nutrient film technique. Int. J. Agric. Biol. 12: 377-380.
Altman, A. 2003. From plant tissue culture to biotechnology: scientific revolutions, biotic stress tolerance and forestry. In vitro Cell. Dev. Biol. Plant. 39: 75-84.
Caswell, K. L., N. L. Leung. and R. N. Chibbar. 2000. An efficient method for in vitro regeneration from immature inflorescence explants of Canadian wheat cultivars. Plant cell, tissue and organ cult. 60(1): 69-73.
Delporte, F., O. Mostade. and J. Jacquemin. 2001. Plant regeneration through callus initiation from thin mature embryo fragments of wheat. Plant Cell, Tissue and Organ Cult. 67(1): 73-80.
Gandonou C. B, T. Errabii, J. Abrini, M. Idaomar. and N. S. Senhaji. 2006. Selection of callus cultures of sugarcane (Saccharum sp.) tolerant to NaCl and their response to salt tolerance. Plant Cell Tissue Organ Cult. 87: 9-16.
Mohammadnejad, M., Z. Khodarahmpour. and Sh. Sadat. 2016. Evaluation of Tolerance of CP73-21 sugarcane callus to salinity. Elixir Agri. 96: 41528-41529.
Munns, R. 2002. Comparative Physiology of Salt and Water Stress. Plant Cell Environ. 28: 239-250.
Rahimi, E. 2016. The evaluation of induced drought commercial sugarcane cultivar CP69-1062 via somaclonal variation. Msc. Thesis of Plant Breeding. Islamic Azad University Ahvaz Branch. 56 pp. (Abstract in English)
Sadat, Sh., M. Soltani Hoveize, M. Mojadam. and S. K. Marashi. 2013. Somaclonal variation and the study of its isozyme electrophoretic pattern in sugarcane variety NCO31. Afr. J. Agri. Res. 8(46): 5814-5820.
Saharan, V., R. C. Yadav, N. R. Yadav. and B. P. Chapagain. 2004. High frequency plant regeneration from desiccated calli of indica rice (Oryza sativa L.). Afr. J. Biotech. 3(5): 256-259.
Shomeili, M., M. Nabipour, M. Meskarbashee. and H. R. Memari. 2011. valuation of sugarcane (Saccharum officinarum L.) somaclonal variants tolerance to salinity in vitro and in vivo cultures. Afr. J. Biotech. 10 (46): 9337-9343.
Wahid, A., H. Sabir, M. Farooq, A. Ghazanfar. and R. Rasheed. 2009. Role of nodal bud and sprout tissue nutrients in sprout establishment, growth and salt tolerance of sugarcane. Crop Past. Sci. 60: 453-462.