Synthesis of Silica Nanoparticles Coated by Poly Dopamine Imprinted Polymer for the Determination of Testosterone in Capsules
Payam Tabar
1
(
Department of Chemical Engineering, Damghan Branch, Islamic Azad University, Damghan, Iran
)
Hamid Hashemi-Moghaddam
2
(
Department of Chemistry, Damghan Branch, Islamic Azad University, Damghan, Iran
)
Homa Baghaei
3
(
Department of Food Science and Technology, Damghan Branch, Islamic Azad University, Damghan, Iran
)
Keywords: Molecular imprint polymer, Solid-phase extraction, Testosterone, Polydopamine,
Abstract :
This study outlines the synthesis and initial evaluation of poly dopamine molecularly imprinted polymers (MIPs) designed for the selective extraction of testosterone. The resulting polymer was utilized as a solid-phase extraction (SPE) sorbent material. The molecularly imprinted polymer was created using a reaction mixture that included silica, the template (testosterone), a functional monomer (dopamine), and water as the solvent. Core-shell structure MIP beads were formed through precipitation and oxidative polymerization. During the polymerization process, a complex was formed between the template and the functional monomer, resulting in a three-dimensional polymer network that encapsulated the template molecules upon completion of polymerization. Subsequently, the template molecules were removed through washing. A control polymer, which was a non-imprinted polymer, was synthesized under similar conditions without the template to facilitate a comparative analysis of performance. Characterization of the synthesized polymer was conducted using Fourier Transform Infrared Spectroscopy (FT-IR), Scanning Electron Microscopy (SEM), Transmission Electron Microscopy (TEM), and Thermogravimetric Analysis (TGA). Optimization of parameters such as pH, extraction time, and adsorption capacity was performed. Ultimately, the concentration of testosterone in urine samples was quantified using UV-Vis spectrophotometry following preconcentration with the synthesized MIP. The results obtained demonstrate the effectiveness of the synthesized polymer in extracting testosterone from real samples.
1. Arévalo F.J., Messina G.A., Molina P.G., Zón M.A., Raba J., Fernández H., 2010. Determination of Testosterone (P4) from bovine serum samples using a microfluidic immunosensor system. Talanta. 80(5), 1986-1992.
2. Eddy R., Clark P., 1987. Oestrus prediction in dairy cows using an ELISA Testosterone test. The veterinary Record. 120(2), 31-34.
3. Khatun S., Nara S., Tripathi V., Rangari K., Chaube S. K., Kariya K. P., Kumar S., Shrivastav T. G., 2009. Development of ELISA for Measurement of Testosterone Employing 17-α-OH-P-HRP as Enzyme Label. Journal of Immunoassay and Immunochemistry®. 30(2), 186-196.
4. Hashemi-Moghaddam H., Hagigatgoo M., 2015. Nonderivatized sarcosine analysis by gas chromatography after solid-phase microextraction by newly synthesized monolithic molecularly imprinted polymer. Chromatographia. 78(19), 1263-1270.
5. Shrivastav T.G., Chaube S.K., Kariya K.P., Prasad P.K., Kumar D., 2013. Influence of different length spacers containing enzyme conjugate on functional parameters of Testosterone ELISA. Journal of Immunoassay and Immunochemistry. 34(1), 94-108.
6. Enbergs H., 1984 [Control of Testosterone level in domestic animals using customary RIA [radio-immuno-assay] tests].
7. Perotti L., Enne G., Fossati P., Curto G., 1981. [Preliminary trials on the validity of direct radio-immuno-assay in milk Testosterone determination [cattle pregnancy diagnosis]. Atti della Societa Italiana delle Scienze Veterinarie (Italy)
8. Pucci V., Bugamelli F., Mandrioli R., Luppi B., Raggi M.A., 2003. Determination of Testosterone in commercial formulations and in non conventional micellar systems. Journal of Pharmaceutical and Biomedical Analysis. 30(5), 1549-1559.
9. Ricanyová J., Gadzała-Kopciuch R., Reiffova K., Bazel Y., Buszewski B., 2010. Molecularly imprinted adsorbents for preconcentration and isolation of Testosterone and testosterone by solid phase extraction combined with HPLC. Adsorption. 16(4-5), 473-483.
10. Short R., LEVETT I., 1962. The fluorimetric determination of Testosterone in human plasma during pregnancy and the menstrual cycle. Journal of Endocrinology. 25(2), 239-244.
11. Heap R., 1964. A fluorescence assay of Testosterone . Journal of Endocrinology. 30(3), 293-305.
12. Saber‐Tehrani M., Hashemi‐Moghaddam H., Husain S. W., Zare K., 2007. Solid‐Phase Extraction and Spectrophotometric Determination of Mercury with 6‐Mercaptopurine in Environmental Samples. Annali di Chimica. 97(8), 675-683.
13. Petrović M., Gonzalez S., Barceló D., 2003. Analysis and removal of emerging contaminants in wastewater and drinking water. TrAC Trends in Analytical Chemistry. 22(10), 685-696.
14. Saber-Tehrani M., Hashemi-Moghaddam H., Givianrad M.H., Abroomand-Azar P., 2006. Methylmercury determination in biological samples using electrothermal atomic absorption spectrometry after acid leaching extraction. Analytical and Bioanalytical Chemistry. 386, 1407-1412.
15. Hashemi-Moghaddam H., Noshiri Z., 2015. Removal of cyanide and zinc–cyanide complex with malachite green functionalized amberlite XAD-4 resin from electroplating wastewater. Desalination and Water Treatment. 53(9), 2481-2488.
16. Hashemi-Moghaddam H., Haghiri H.K., 2016. Preconcentration of some heavy metals by Amberlite XAD-4 functionalized with Phenanthroline and investigation of microwave radiation effect on kinetic of adsorption. Desalination and Water Treatment. 57(4), 1705-1712.
17. Alpdoğan G., 2016. Solid phase extraction of Cu (II), Ni (II), Co (II), and Fe (III) ions in water samples using salicylaldehyde benzoylhydrazone on Amberlite XAD-4 and their determinations by flame atomic absorption spectrometry. Toxicological & Environmental Chemistry. 98(2), 179-188.
18. Hosseini-Bandegharaei A., Allahabadi A., Rahmani-Sani A., Rastegar A., Khamirchi R., Mehrpouyan M., Hekmat-Shoar R., Pajohankia Z., 2016. Thorium removal from weakly acidic solutions using titan yellow-impregnated XAD-7 resin beads: kinetics, equilibrium and thermodynamic studies. Journal of Radioanalytical and Nuclear Chemistry. 309(2), 761-776.
19. Hashemi-Moghaddam H., Rahimian M., Niromand B., 2013. Molecularly Imprinted Polymers for Solid-Phase Extraction of Sarcosine as Prostate Cancer Biomarker from Human Urine. Bull. Korean Chem. Soc. 34(8), 2331.
20. Hashemi-Moghaddam H., Shakeri M., 2014. Removal of potentioally genotoxic impurity from fluroxamine maleate crude drug by molecularly imprinted polymer. Korean Journal of Chemical Engineering. 31(10), 1898-1902.
21. Hashemi-Moghaddam H., Jedi D.J., 2015. Solid-phase microextraction of chlorpyrifos in fruit samples by synthesised monolithic molecularly imprinted polymer fibres. International Journal of Environmental Analytical Chemistry. 95(1), 33-44.
22. Hashemi-Moghaddam H., Kazemi-Bagsangani S., Jamili M., Zavareh S., 2016. Evaluation of magnetic nanoparticles coated by 5-fluorouracil imprinted polymer for controlled drug delivery in mouse breast cancer model. International Journal of Pharmaceutics. 497(1-2), 228-238.
23. Hashemi-Moghaddam H., Abbasi F., 2015. Synthesis of Molecularly Imprinted Polymers Coated on Silica Nanoparticles for Removal of P-Nitrophenol from Crude Pharmaceuticals. Pharmaceutical Chemistry Journal. 49(4), 280-286.
24. Hashemi-Moghaddam H., Zavareh S., Gazi E.M., Jamili M., 2018. Assessment of novel core–shell Fe3O4@ poly l‑DOPA nanoparticles for targeted Taxol® delivery to breast tumor in a mouse model. Materials Science and Engineering: C. 93, 1036-1043.