In-vivo and In-vitro Evaluation for Memory Enhancing Activity of Some Isoflavonoids by Suitable Animal Models
Subject Areas : Journal of Chemical Health RisksQutaiba A. Qasim 1 , Ahmed A. A. Alsaad 2 , H.N.K. AL-Salman 3
1 - College of Pharmacy, Al-Ayen Universite, Thi Qar, Iraq
2 - Department of Pharmaceutics, College of Pharmacy, Basrah University, Basrah, Iraq
3 - Department of Pharmaceutical Chemistry, College of Pharmacy, University of Basrah, Basrah, Iraq
Keywords: EL, TSTQ, Isoflavonoids, Genistein, Glycitein, Cognitive dysfunction,
Abstract :
In-vivo and In-vitro evaluation of isoflavonoids for improving memory activity in animal models were completed using this AIM. The materials and methods are interchangeable." As recommended by the OECD, revised draught tenet 423, the acute oral toxicity study has been undertaken. During the earlier stage of experimentation, it was observed that trying out knowledge of and reminiscence of mice happened using the Morris water maze. Two reverse open fingers are joined with two similarly sized closed fingers with a 30 cm wide excess wall to create the extended plus-maze tools. In other words, the fingers are connected to Central Square. Just after the fifteenth day, the Morris water maze was utilised, followed by the sacrifice of the animals after the sixteenth day by performing cervical dislocation. During previous iterations of Acetyl Cholinesterase Activity estimation, absolute genius was employed cautiously. Results reveal that breakthrough latency and time is taken in the goal quadrant are linked to learning and reminiscence. The EL declination and the TSTQ increase are demonstrated in the Morris water maze, which revealed an improvement in memory and recall and reminiscence. There was a broad (at the 99.9% confidence level) increase in the share of open palms and the time spent in open arms (associated with administering a 1 mg kg-1 dosage of diazepam, p. o.) Besides, a large (at the 99.9% confidence level) reduces the number of time animals spends in restricted arms and the quantity of entry to restricted areas. These characteristics will also be used to understand better diseases with a higher level of cellular and molecular complexity.
1. Lawrence E., Vegvari C., Ower A., Hadjichrysanthou C., De Wolf F., Anderson RM., 2017. A Systematic Review of Longitudinal Studies Which Measure Alzheimer's Disease Biomarkers. J Alzheimers Dis. 59, 359-379.
2. Esquerda-Canals G., Montoliu-Gaya L., Güell-Bosch J., Villegas S., 2017. Mouse Models of Alzheimer's Disease. J Alzheimers Dis. 57, 1171-1183.
3. Kanwal A., Mehla J., Kuncha M., Naidu V.G.M., 2010. Anti-Amnesic activity of Vitex negundo in Scopolamine induced Amnesia in Rats. Pharmacology and Pharmacy. 1, 1-8.
4. Nabi N.U., Neeraj K., Ravi K., Preeti K., 2013. Natural Remedies for Improving Learning and Memory-Review. Int J Pharm. Phytopharmacol Res. 3,161-165.
5. OECD Guidelines 2001. “Guidance document on acute oral toxicity testing” Series on testing and assessment No. 23, Organization for Economic Co‑operation and Development, OECD Environment, health and safety publications, Paris Available from: http://www.Oecd.org/ehs [accessed 20 March on 2010].
6. Khumpirapang N., Chaichit S., Jiranusornkul S., Pikulkaew S., Müllertz A., Okonogi S., 2018. In vivo anesthetic effect and mechanism of action of active compounds from Alpinia galanga oil on Cyprinus carpio (koi carp). Aquaculture. 496, 176-184.
7. Morris R.,1984. Developments of a water-maze procedure for studying spatial learning in the rat. Journal of Neuroscience Methods. 11, 47-60.
8. Iqbal I., Khanam R., Hassan S., Roou A., Moshahid Alam M., Islam A., Thakur S., Athara F., New insights into the antioxidant and apoptotic potential of Glycyrrhiza glabra L. during hydrogen peroxide mediated oxidative stress: An in vitro and in silico evaluation. Biomed Pharmacother. 94, 265-279.
9. Goverdhan P., Sravanthi A., Mamatha T., 2012 Neuroprotective effects of meloxicam and selegiline in scopolamine-induced cognitive impairment and oxidative stress. Int J Alzheimers Dis. 2012, 407-416.
10. Barulli M.R., Piccininni M., Di C., Musar C., Grasso A., Tursi M., 2019. Episodic memory and learning rates in amyotrophic lateral sclerosis without dementia. Cortex. 17, 257-65.
11. Samuel N., Taub A.H., Paz R., Raz A., 2018. Implicit aversive memory under anaesthesia in animal models: a narrative review. Br J Anaesth. 121, 219-232.
12. Herszage J., Censor N., 2018. Modulation of learning and memory: a shared framework for interference and generalization. Neuroscience. 392, 270-280.
13. Hasselmo M.E., 2006. The Role of Acetylcholine in Learning and Memory. Current Opinion in Neurobiology. 16, 710–715.
14. Elisa T., Paula A., Laiz P., 2018. Taraxerol as a possible therapeutic agent on memory impairments and Alzheimer’s disease, Effects against scopolamine and streptozotocin-induced cognitive dysfunctions. Steroids. 132, 5-11.
15. Kharat M., Parulkar G., 2016. Saraswatarishta; A Miraculous Rasayana.World Journal of Pharmaceutical Research. 5, 358-361.
16. Anadozie S.O., Akinyemi J.A., Adewale O.B., Isitua C.C., 2019. “Prevention of short-term memory impairment by Bryophyllum pinnatum (Lam.) Oken and its effect on acetylcholinesterase changes in CCl4-induced neurotoxicity in rats,” Journal of Basic and Clinical Physiology and Pharmacology. 1–10.
17. Nair S., Dixit S., Ganesh N., 2017. “Comparative study of invitro antioxidant potential of crude extracts of bryophyllum pinnatum [(Lam) oken] leaves in different solvents and the invitro hypoglycemic potential of its hydroalcoholic extract,” Journal of Chemical and Pharmaceutical Research. 9, 237–243.
18. Ghoshal S., Dutta S.K., 1972. Erythrina chemical and pharmacological evaluation II Alkaloids of Erythina variegata. J Pharm Sci. 61, 1274-1277.
19. Onoja S.O., Ihejirika G.Q., Nwankudu O.N., Omeh Y.N., Ezeja M.I., 2018. “Antidiarrheal and antioxidant activities of methanol extract of bryophyllum pinnatum leaf harvested from south-eastern Nigeria in mice. Journal of Pharmaceutics. 1–6.
20. Kulkarni P.D., Ghaisas M.M., Chivate N.D., Sankpal P.S. 2011. Memory enhancing activity of Cissampelos pareira in mice. International Journal of Pharmacy and Pharmaceutical Sciences. 3, 206-211.
21. Watanabe T., Yamagata N., Takasaki K., Sano K., Hayakawa K., Katsurabayashi S., 2009. Decreased acetylcholine release is correlated to memory impairment in the Tg2576 transgenic mouse model of Alzheimer’s disease. Brain Research. 1249, 222-228.
22. Indumathy S., Kavimani S., Raman K.V., 2010. Role of angiotensin antagonists in memory enhancement. International Journal of Pharma and Bio Sciences. 1, 1-6.
23. Esquerda-Canals G., Montoliu-Gaya L., Güell-Bosch J., Villegas S., 2017. Mouse Models of Alzheimer's Disease. J Alzheimers Dis. 57, 1171-1183.
24. Tang X., Xu C., Yagiz Y., Simonne A., Marshall M.R., 2018. Phytochemical profiles, and antimicrobial and antioxidant activities of greater galangal [Alpinia galanga (Linn.) Swartz.] flowers. Food Chem. 255, 300-308.
25. Khumpirapang N., Chaichit S., Jiranusornkul S., Pikulkaew S., Müllertz A., Okonogi S., 2018. In vivo anesthetic effect and mechanism of action of active compounds from Alpinia galanga oil on Cyprinus carpio (koi carp). Aquaculture. 496, 176-184.
26. Yang R., Yuan B.C., Ma Y.S., Zhou S., Liu Y. , 2017. The anti-inflammatory activity of licorice, a widely used Chinese herb. Pharm Biol. 55, 5-18.