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Manuscript ID : JCHR-2206-1577 (R1) Visit : 142 Page: 423 - 439

10.22034/jchr.2022.1961632.1577

Article Type: Original Research

Exposure to Insecticide Mixture of Cypermethrin and Dichlorvos Induced Neurodegeneration by Reducing Antioxidant Capacity in Striatum

Subject Areas : Journal of Chemical Health Risks

Princewill  Udodi 1 (Department of Anatomy, Faculty of Basic Medical Sciences, Nnamdi Azikiwe University, Nigeria)
Tobechi  ANONYE 2 (Department of Anatomy, Faculty of Basic Medical Sciences, Nnamdi Azikiwe University, Nigeria)
Damian  Ezejindu 3 (Department of Anatomy, Faculty of Basic Medical Sciences, Nnamdi Azikiwe University, Nigeria)
Joshua  ABUGU 4 (Department of Anatomy, Faculty of Basic Medical Sciences, Nnamdi Azikiwe University, Nigeria)
Chizubelu  OMILE 5 (Department of Anatomy, Faculty of Basic Medical Sciences, Nnamdi Azikiwe University, Nigeria)
Ifechukwu  Obiesie 6 (Department of Anatomy, Faculty of Basic Medical Sciences, Nnamdi Azikiwe University, Nigeria)
Roseline  OGWO 7 (Department of Anatomy, Faculty of Basic Medical Sciences, Nnamdi Azikiwe University, Nigeria)
Chukwudumebi  Abattam 8 (Department of Anatomy, Faculty of Basic Medical Sciences, Nnamdi Azikiwe University, Nigeria)
Darlington  AKUKWU 9 (Department of Human Anatomy, Imo State University, Owerri, Imo State, Nigeria)
Godwin  Uloneme 10 (Department of Human Anatomy, Imo State University, Owerri, Imo State, Nigeria)
Charles  Oyinbo 11 (Department of Anatomy, Niger Delta University, Amassoma, Bayelsa State, Nigeria)

Received: 2022-06-22 Accepted : 2022-10-08 Published : 2023-09-01

Keywords: Malondialdehyde, Histopathology, Cypermethrin, Dichlorvos, Pyrethroid, Chromatolysis,

Abstract :

To evaluate the effect of cypermethrin (CP) and dichlorvos (2,2-dichlorovinyl dimethyl phosphate, DDVP) on the striatum of adult Wistar rats. Thirty-two animals were grouped into 4; group A (control) inhaled fresh air, and groups B, C, and D were exposed to a formulation of 5 mm-1 (4.4 ppm) of dichlorvos and 10 mm-1 (8.7 ppm) of cypermethrin insecticide for 2hrs/day, 3hrs/day and 4hrs/day respectively. We utilized the wire suspension test to demonstrate the neurobehavioral changes across the four groups of animals to identify the animal groups that have lost their motor function. Following the neurobehavioral test, the animals were weighed, anesthesized and dissected for brain tissue harvesting. Half of the brain tissue was frozen for biochemical analysis while the other part was fixed in 10% Neutral Buffered Formalin for two days and grossed to isolate the brain tissue of interest for histopathology. The results from the neurobehavioral studies show a significant decrease in motor function of the experimental groups. There was a significant elevation in the malondialdehyde and glucose levels of all the exposed groups, while their various antioxidant levels decreased significantly (p<0.05). Histopathological features were observed across the exposed groups ranging from the presence of vacuolated neuronal cells, neuronal cell shrinkage, and chromatolysis, which characterize the neurodegenerative effect of cypermethrin and dichlorvos on the striatum. This study indicates that a combined administration of cypermethrin and dichlorvos exerts a neurodegenerative effect on the striatum of adult Wistar rats.

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