Carvacrol Attenuates Disrupted Lipid Profile Induced by Organophosphates in Male Wistar Rat: a Comparative Toxicity
Subject Areas : Journal of Chemical Health Risks
Ali Salari
1
,
Kambiz Roshanaei
2
,
Bahram Rasoulian
3
,
Javad Khalili Fard
4
1 - Department of Physiology, Faculty of Sciences, Qom Branch, Islamic Azad University, Qom, Iran
2 - Department of Biology, Faculty of Sciences, Qom Branch, Islamic Azad University, Qom, Iran
3 - Hepatitis Research Center, Lorestan University of Medical Sciences, Khorramabad, Iran
4 - Razi Herbal Medicines Research Center, Lorestan University of Medical Sciences, Khorramabad, Iran|Department of Pharmacology and Toxicology, Faculty of Pharmacy, Tabriz University of Medical Sciences, Tabriz, Iran
Keywords: Carvacrol, Cholesterol, Rat, Malathion, Triglyceride, Parathion,
Abstract :
Many people in agricultural industry are nowadays struggling with protecting their products utilizing pesticides. Pesticides, such as organophosphate (OPE) insecticides, may remain on agricultural products as pesticide residues. Malathion (MTN) is an OPE widely used around the world. Some OPEs, such as parathion (PTN), are more toxic pesticides and have been restricted. Carvacrol (CRL) is a major component of Satureja khuzestanica essential oil, whichexerted protective effects against toxicity of chemicals. OPEs can alter lipid profile. In addition, lipid profile may alter due to certain disorders, such as nephrotic syndrome. The present study aimed to investigate the effects of coadministration of CRL and the effect of these two pesticides on serum acetylcholinesterase (AchE) activity and lipid profile in male wistar rat. Coadministration of CRL and PTN, but not MTN, significantly decreased serum AchE activity in comparison with the group receiving OPE. Serum levels of cholesterol and triglyceride were analyzed after 10 days of administration of the chemicals. Malathion and PTN significantly increased cholesterol and triglyceride. However, administration of CRL modified lipid profile (P < 0.05). It was concluded that CRL could be considered as a drug to treat lipid profile alteration and owing to the beneficial effects as well as inhibition of acetylcholine, it could be considered as a component of OPE pesticide.
1. Tsakiris I.N., Toutoudaki M., Kokkinakis M., Paraskevi M., Tsatsakis A.M., 2011. A risk assessment study of Greek population dietary chronic exposure to pesticide residues in fruits, vegetables and olive oil. pesticides-formulations, effects, fate. Stoytcheva, M.(Ed.). InTech, 253-268.
2. Wiersielis K., Adams S., Yasrebi A., Conde K., Roepke T., 2020. Maternal exposure to organophosphate flame retardants alters locomotor and anxiety-like behavior in male and female adult offspring. Hormones and Behavior, 122, 104759.
3. Al-Othman A.M., Al-Numair K.S., El-Desoky G.E., Yusuf K., Al Othman Z. A., Aboul-Soud M.A., Giesy J.P., 2011. Protection of-tocopherol and selenium against acute effects of malathion on liver and kidney of rats. African Journal of Pharmacy and Pharmacology. 5(10), 1263-1271.
4. Yokota K., Fukuda M., Katafuchi R., Okamoto T., 2017. Nephrotic syndrome and acute kidney injury induced by malathion toxicity. Case Reports. 2017, bcr-2017-220733.
5. Céspedes J., Pellegrino J., Ferrer M., Rodríguez J., Koch O., EA R.G., 1979. Hepatic function in vivo and in the isolated liver of rats poisoned with parathion. Acta gastroenterologica Latinoamericana. 9(2), 67-72.
6. Fuentes-Delgado V.H., Martínez-Saldaña M.C., Rodríguez-Vázquez M.L., Reyes-Romero M.A., Reyes-Sánchez J.L., Jaramillo-Juárez F., 2018. Renal damage induced by the pesticide methyl parathion in male Wistar rats. Journal of Toxicology and Environmental Health, Part A. 81(6), 130-141.
7. Kiss Z., Fazekas T., 1979. Arrhythmias in organophosphate poisonings. Acta Cardiologica. 34(5), 323-330.
8. Khairy M., Ayoub H. A., Banks C.E., 2018. Non-enzymatic electrochemical platform for parathion pesticide sensing based on nanometer-sized nickel oxide modified screen-printed electrodes. Food Chemistry. 255, 104-111.
9. Aggarwal N., Gupta R., 2015. Antigenotoxic potential of curcumin and carvacrol against malathion-induced DNA damage in cultured human peripheral blood and its relation to GSTM1 and GSTT1 polymorphism. Biomarkers and Genomic Medicine. 98-104.
10. Al-Attar, Atef M., 2010. Physiological and histopathological investigations on the effects of 𝛼-lipoic acid in rats exposed to malathion. Journal of Biomedicine and Biotechnology.;2010:203503
11. Ince S., Arslan-Acaroz D., Demirel H.H., Varol N., Ozyurek H.A., Zemheri F., Kucukkurt I., 2017. Taurine alleviates malathion induced lipid peroxidation, oxidative stress, and proinflammatory cytokine gene expressions in rats. Biomedicine & Pharmacotherapy. 96, 263-268.
12. Jalili C., Roshankhah S., Moradi Y., Salahshoor M.R., 2018. Resveratrol attenuates malathion‑induced renal damage by declining oxidative stress in rats. International Journal of Pharmaceutical Investigation. 8(4), 192-199.
13. Mohammadzadeh L., Abnous K., Razavi B.M., Hosseinzadeh H., 2020. Crocin-protected malathion-induced spatial memory deficits by inhibiting TAU protein hyperphosphorylation and antiapoptotic effects. Nutritional Neuroscience. 23(3), 221-236.
14. Delfan B., Kheirandish F., Chegeni R., Jabari M., Ebrahimzadeh F., Rashidipour M., 2016. The cytotoxic and antileishmanial effects of satureja khuzestanica essential oil. Herbal Medicines Journal. 1(1), 7-11.
15. Jukic M., Politeo O., Maksimovic M., Milos M., Milos M., 2007. In vitro acetylcholinesterase inhibitory properties of thymol, carvacrol and their derivatives thymoquinone and thymohydroquinone. Phytotherapy Research. 21(3), 259-261.
16. Askin H., Yildiz M., Ayar A., 2017. Effects of Thymol and Carvacrol on Acetylcholinesterase from Drosophila melanogaster. Acta Phys Pol. 132, 720-722.
17. Chen H.W., He X.H., Yuan R., Wei B.J., Chen Z., Dong J.X., Wang J., 2016. Sesquiterpenes and a monoterpenoid with acetylcholinesterase (AchE) inhibitory activity from Valeriana officinalis var. latiofolia in vitro and in vivo. Fitoterapia. 110, 142-149.
18. Kurt B.Z., Gazioglu I., Dag A., Salmas R.E., Kayık G., Durdagi S., Sonmez F., 2017. Synthesis, anticholinesterase activity and molecular modeling study of novel carbamate-substituted thymol/carvacrol derivatives. Bioorg Med Chem. 25(4), 1352-1363.
19. Lee K.W., Everts H., Kapperst H.J., Yeom K.H., Beynen A.C., 2003. Dietary Carvacrol Lowers Body Weight Gain but Improves Feed Conversion in Female Broiler Chickens. Journal of Applied Poultry Research. 12(4), 394-399.
20. Alavanja MC., 2009. Introduction: pesticides use and exposure extensive worldwide. Rev Environ Health. 24(4):303-309.
21. Loizou E., Karelakis C., Galanopoulos K., Mattas K., 2019. The role of agriculture as a development tool for a regional economy. Agricultural Systems. 173, 482-490.
22. Yu I.S., Lee J.S., Kim S.D., Kim Y.H., Park H.W., Ryu H.J., Lee J.H., Lee J.M., Jung K., Na C., 2017. Monitoring heavy metals, residual agricultural chemicals and sulfites in traditional herbal decoctions. BMC Complementary and Alternative Medicine. 17(1), 1-9.
23. Olakkaran S., Purayil A.K., Antony A., Mallikarjunaiah S., Puttaswamygowda G.H., 2020. Oxidative stress-mediated genotoxicity of malathion in human lymphocytes. Mutation Research/Genetic Toxicology and Environmental Mutagenesis. 849, 503138.
24. Poomagal S., Sujatha R., Kumar P.S., Vo D.V.N., 2021. A fuzzy cognitive map approach to predict the hazardous effects of malathion to environment (air, water and soil). Chemosphere. 263, 127926.
25. Salari A., Roshanaei K., Rasoulian B., Fard J.K., 2021. Carvacrol loaded beta cyclodextrin-alginate-chitosan based nanoflowers attenuates renal toxicity induced by malathion and parathion: A comparative toxicity. Pesticide Biochemistry and Physiology. 172, 104747.
26. Calaf G., Roy D., 2008. Cancer genes induced by malathion and parathion in the presence of estrogen in breast cells. International Journal of Molecular Medicine. 21(2), 261-268.
27. Calaf G.M., Roy D., 2007. Gene expression signature of parathion-transformed human breast epithelial cells. International Journal of Molecular Medicine. 19(5), 741-750.
28. Calaf G., Roy D., 2007. Human drug metabolism genes in parathion-and estrogen-treated breast cells. International Journal of Molecular Medicine. 20(6), 875-881.
29. Naraharisetti S.B., Aggarwal M., Ranganathan V., Sarkar S.N., Kataria M., Malik J.K., 2009. Effects of simultaneous repeated exposure at high levels of arsenic and malathion on hepatic drug-biotransforming enzymes in broiler chickens. Environmental Toxicology and Pharmacology. 28(2), 213-218.
30. Adigun A.A., Wrench N., Levin E.D., Seidler F.J., Slotkin T.A., 2010. Neonatal parathion exposure and interactions with a high-fat diet in adulthood: Adenylyl cyclase-mediated cell signaling in heart, liver and cerebellum. Brain Research Bulletin. 81(6), 605-612.
31. Fuentes-Delgado V.H., Martínez-Saldaña M.C., Rodríguez-Vázquez M.L., Reyes-Romero M.A., Reyes-Sánchez J.L., Jaramillo-Juárez F., 2018. Renal damage induced by the pesticide methyl parathion in male Wistar rats. Journal of Toxicology and Environmental Health - Part A: Current Issues. 81(6), 130-141.
32. Howard M.D., Pope C.N., 2002. In vitro effects of chlorpyrifos, parathion, methyl parathion and their oxons on cardiac muscarinic receptor binding in neonatal and adult rats. Toxicology. 170(1-2), 1-10.
33. Goldsmith M., Ashani Y., Margalit R., Nyska A., Mirelman D., Tawfik D.S., 2016. A new post-intoxication treatment of paraoxon and parathion poisonings using an evolved PON1 variant and recombinant GOT1. Chemico-Biological Interactions. 259, 242-251.
34. Hashemi S.M.B., Khodaei D., 2020. Antimicrobial activity of Satureja Khuzestanica Jamzad and Satureja bachtiarica Bunge essential oils against Shigella flexneri and Escherichia coli in table cream containing Lactobacillus plantarum LU5. Food Science & Nutrition. 8(11), 5907-5915.
35. Askin H., Yildiz M., Ayar A., 2017. Effects of thymol and carvacrol on acetylcholinesterase from Drosophila melanogaster. Acta Physica Polonica A. 132(3), 720-722.
36. Tyagi R., 2011. Pharmacological Studies of Disorders In Lipid Profile And Kidney Dysfunctions Due To Methyl Parathion Toxicity On Fresh Water Fish, Clarias Batrachus (Linn.). Biochemical and Cellular Archives. 11(1), 131-133.
37. Tyagi R., 2012. Pathological effects of methyl parathion on lipid and cholesterol of fresh water fish, Channa punctatus (Bloch). Biochemical and Cellular Archives. 12(1), 105-110.
38. Abdel-Daim M.M., Abushouk A.I., Bungău S.G., Bin-Jumah M., El-kott A.F., Shati A.A., Aleya L., Alkahtani S., 2020. Protective effects of thymoquinone and diallyl sulphide against malathion-induced toxicity in rats. Environmental Science and Pollution Research. 27(10), 10228-10235.
39. Aristatile B., Ai-Numair K.S., Veeramani C., Pugalendi K.V., 2009. Antihyperlipidemic effect of carvacrol on d-galactosamine-induced hepatotoxic rats. Journal of Basic and Clinical Physiology and Pharmacology. 20(1), 15-28.
40. Mostafazadeh K., Shahryar H.A., Shayegh J., 2012. Influence of Satureja sahandica Bornm extract on darkens liver, lowers blood cholesterol, proportional liver and abdominal fat weight in broiler chickens. European Journal of Experimental Biology. 2(1), 283-287.
41. Masouri B., Sallary S., Khosravinia H., Tabatabaei Vakili S., Mohammadabadi T., 2017. Effect of dietary fat source and Satureja Khuzistanica essential oils on performance, blood lipid constituents, cholesterol content and lipid stability of meat in broiler chicken under heat stress. Animal Production. 19(1), 201-212.