Elaeagnus angustifolia L. Whole Fruit Ethanolic Extract: Phytochemical Composition and Antimicrobial Effects

Subject Areas : Journal of Chemical Health Risks

Leila  Monjazeb Marvdashti ¹ (Department of Food Science and Technology, Faculty of Agriculture, Ferdowsi University of Mashhad, Mashhad, Iran)
Maryam  Mohammadbeigi ² (Department of microbiology& Immunology, Qazvin University of Medical Sciences, Qazvin, Iran)
Samaneh  Arab ³ (Nervous System Stem Cells Research Center, Semnan University of Medical Sciences, Semnan, Iran)
Arezoo  Ebrahimi ⁴ (semnan)
Arezou  Rezaei ⁵ (School of Biology, Damghan University, Damghan, Iran)
Anna  Abdolshahi ⁶ (گروه صنایع غذایی دانشگاه علوم پزشکی سمنان)

Received: 2020-05-04 Accepted : 2022-10-17 Published : 2024-03-01

Keywords: Extract, Antimicrobial, Phytochemical, Elaeagnus angustifolia,

Abstract :

In this research chemical composition and antimicrobial effects of Elaeagnus angustifolia L. (E. angustifolia L.) whole fruit ethanolic extract was investigated on common pathogenic microorganisms (Staphylococcus aureus, Escherichia coli, Enterococcus faecalis, and Candida albicans). The phytochemical composition of E. angustifolia was screened by Gas Chromatography-Mass Spectrometry (GC/MS). The minimum inhibitory concentration (MIC) and the minimal bactericidal concentration (MBC) of the extract were determined using the broth dilution technique. According to the results, 13 major compounds such as flavonoid, aldehyde, alcoholic and fatty acids were identified by GC/MS. In addition, the extract could inhibit the growth of all examined pathogenic strains. The MIC was 3.75 to 1.87 mg ml-1 while maximum activity was found against Staphylococcus aureus. Besides, the MBC was ranging from 7.5 to 3.75 mg ml-1. Since E. angustifolia whole fruit ethanolic extract contains phytochemical compounds and has antimicrobial potential it can be recommended as a natural active agent for application in the food industry.

References:

1. Ahmadiani A., Hosseiny J., Semnanian S., Javan M., Saeedi F., Kamalinejad M., Saremi S., 2000. Antinociceptive and anti-inflammatory effects of Elaeagnus angustifolia fruit extract. Journal of Ethnopharmacology. 72 (1-2), 287-292.


2. Katz G.L., Shafroth P.B., 2003. Biology, ecology and management of Elaeagnus angustifolia L.(Russian olive) in western North America. Wetlands. 23(4), 763-777.


3. Abizov E., Tolkachev O., Mal’Tsev S., Abizova E., 2008. Composition of biologically active substances isolated from the fruits of Russian olive (Elaeagnus angustifolia) introduced in the European part of Russia. Pharmaceutical Chemistry Journal. 42 (12), 696-698.


4. Hosseinzadeh H., Ramezani M., Namjo N., 2003. Muscle relaxant activity of Elaeagnus angustifolia L. fruit seeds in mice. Journal of Ethnopharmacology. 84 (2-3), 275-278.


5. Ramezani M., Hosseinzadeh H., Daneshmand N., 2001. Antinociceptive effect of Elaeagnus angustifolia fruit seeds in mice. Fitoterapia. 72(3), 255-262.


6. Chen Q., Chen J., Du H., Li Q., Chen J., Zhang G., Liu H., Wang J., 2014. Structural characterization and antioxidant activities of polysaccharides extracted from the pulp of Elaeagnus angustifolia L. International Journal of Molecular Sciences. 15(7), 11446-11455.


7. Farzaei M.H., Bahramsoltani R., Abbasabadi Z., Rahimi R., 2015. A comprehensive review on phytochemical and pharmacological aspects of E laeagnus angustifolia L. Journal of Pharmacy and Pharmacology. 67(11), 1467-1480.

 

 

8. Bucur L., Stanciu G., Istudor V., 2007. The GC-MS analysis of Elaeagnus angustfolia L. flowers essential oil. Revista De Chimie-Bucharest-Original Edition. 58(11), 1027-1032.


9. Bucur L., Vlase L., Fodorea C., Istudor V., 2006. The HPLC analysis of polyphenolic compounds traction obtained from flowers and young branches of Elaeagnus angustifolia L. Revista de Chimie. 57(2), 189-192.


10. Du H., Chen J., Tian S., Gu H., Li N., Sun Y., Ru J., Wang J., 2016. Extraction optimization, preliminary characterization and immunological activities in vitro of polysaccharides from Elaeagnus angustifolia L. pulp. Carbohydrate Polymers. 151, 348-357.


11. Ebrahimi A. A., Nikniaz Z., Ostadrahimi A., Mahdavi R., Nikniaz L., 2014. The effect of Elaeagnus angustifolia L. whole fruit and medulla powder on women with osteoarthritis of the knee: A randomized controlled clinical trial. European Journal of Integrative Medicine. 6(6), 672-679.


12. Behbahani B.A., Fooladi A.A.I., 2018. Antibacterial activities, phytochemical analysis and chemical composition Makhlaseh extracts against the growth of some pathogenic strain causing poisoning and infection. Microbial Pathogenesis. 114, 204-208.


13. Nikniaz Z., Ostadrahimi A., Mahdavi R., asghar Ebrahimi A., Nikniaz L., 2014. Effects of Elaeagnus angustifolia L. supplementation on serum levels of inflammatory cytokines and matrix metalloproteinases in females with knee osteoarthritis. Complementary Therapies in Medicine. 22(5), 864-869.


14. Okmen G., Turkcan O., 2013. The antibacterial activity of Elaeagnus angustifolia L. against mastitis pathogens and antioxidant capacity of the leaf methanolic extracts. J Anim Vet Adv. 12(4), 491-496.


15. Bendaikha S., Gadaut M., Harakat D., Magid A., 2014. Acylated flavonol glycosides from the flower of Elaeagnus angustifolia L. Phytochemistry.103, 129-136.


16. Dehghan M.H., Soltani J., Farnad M., Kalantar E., Kamalinejad M., Khodaii Z., Hatami S., Natanzi M.M., 2014. Characterization of an antimicrobial extract from elaeagnus angustifolia. International Journal of Enteric Pathogens. 2(3), 1-4.


17. Ab Rahim N., Zakaria N., Dzulkarnain S.M.H., Othman N., Abdulla M.A., 2019. Antibacterial activity of Alstonia angustifolia leaf extract against Staphyloccoccal and Bacilli. Biomedical Research. 30(1), 11-15.


18. Okmen G., Turkcan O., 2014. A study on antimicrobial, antioxidant and antimutagenic activities of Elaeagnus angustifolia L. leaves. African Journal of Traditional, Complementary and Alternative Medicines. 11(1), 116-120.


19. Bahraminejad S., Amiri R., Abbasi S., 2015. Anti-fungal properties of 43 plant species against Alternaria solani and Botrytis cinerea. Archives of Phytopathology and Plant Protection. 48(4), 336-344.


20. Abdolshahi A., Shabani A. A., Mortazavi S. A., Marvdashti L.M., 2018. Aflatoxin binding efficiency of Saccharomyces cerevisiae mannoprotein in contaminated pistachio nuts. Food Control. 87, 17-21.


21. Abdolshahi A., Marvdashti L.M., Salehi B., Sharifi‐Rad M., Ghobakhloo S., Iriti M., Sharifi‐Rad J., 2019. Antifungal activities of coating incorporated with Saccharomyces cerevisiae cell wall mannoprotein on Aspergillus flavus growth and aflatoxin production in pistachio (Pistacia vera L.). Journal of Food Safety. 39(2), e12608.


22. Abdolshahi A., Naybandi-Atashi S., Heydari-Majd M., Salehi B., Kobarfard F., Ayatollahi S.A., Ata A., Tabanelli G., Sharifi-Rad M., Montanari C., 2018. Antibacterial activity of some Lamiaceae species against Staphylococcus aureus in yoghurt-based drink (Doogh). Cellular and Molecular Biology. 64(8), 71-77.


23. Society A. AOCS official method Ce 1f-96., 2002, (77), 457-462.


24. Hameed I.H., Jasim H., Kareem M.A., Hussein A.O., 2015. Alkaloid constitution of Nerium oleander using gas chromatography-mass spectroscopy (GC-MS). Journal of Medicinal Plants Research. 9(9), 326-334.


25. Akbolat D., Ertekin C., Menges H., Guzel E., Ekinci K., 2008. Physical and nutritional properties of oleaster (Elaeagnus angustifolia L.) growing in Turkey. Asian Journal of Chemistry. 20(3), 2358-2366.


26. Wang Y., Guo T., Li J.Y., Zhou S.Z., Zhao P., Fan M.T., 2013. Four flavonoid glycosides from the pulps of Elaeagnus angustifolia and their antioxidant activities. Advanced Materials Research. 16-20.


27. Panahi Y., Alishiri G. H., Bayat N., Hosseini S. M., Sahebkar A., 2016. Efficacy of Elaeagnus angustifolia extract in the treatment of knee osteoarthritis: a randomized controlled trial. Excli Journal. 15, 203-210.


28. Sabir M.S., Ahmad D.S., Hussain I.M., Tahir K.M., 2007. Antibacterial activity of Elaeagnus umbellata (Thunb.) a medicinal plant from Pakistan. Saudi Medical Journal. 28(2), 259-263.


29. Aneja K.R., Sharma C., Joshi R., 2011. In vitro efficacy of amaltas (Cassia fistula L.) against the pathogens causing otitis externa. Jundishapur J Microbiol. 4(3), 175-183


30. Bahorun T., Neergheen V.S., Aruoma O.I., 2005. Phytochemical constituents of Cassia fistula. African Journal of Biotechnology. 4(13), 1530-1540.


31. Draughon F.A., 2004. Use of botanicals as biopreservatives in foods.


32. Rizvi M.M.A., El Hassadi I.M.G., Younis S.B., 2009. Bioefficacies of Cassia fistula: an Indian labrum. African journal of Pharmacy and Pharmacology. 3(6), 287-292.


33. Goyal P., Khanna A., Chauhan A., Chauhan G., Kaushik P., 2008. In vitro evaluation of crude extracts of Catharanthus roseus for potential antibacterial activity. International Journal of Green Pharmacy (IJGP). 2 (3), 176-181


34. Tassou C., Koutsoumanis K., Nychas G.J., 2000. Inhibition of Salmonella enteritidis and Staphylococcus aureus in nutrient broth by mint essential oil. Food Research International. 33(3-4), 273-280.


35. Seyyednejad S.M., Motamedi H., Vafei M., Bakhtiari A., 2014. The antibacterial activity of Cassia fistula organic extracts. Jundishapur Journal of Microbiology. 7(1), 1-5