Synthesis of Hydroxyapatite/Ag/TiO2 Nanotubes and Evaluation of Their Anticancer Activity on Breast Cancer Cell Line MCF-7

Subject Areas : Journal of Chemical Health Risks

Sara Rahimnejad ¹ (Department of Chemistry, College of Basic science, Yadegar-e-Imam Khomeini (RAH( Shahre Rey Branch, Islamic Azad University, Tehran, Iran)
Maryam Bikhof Torbati ² (Department of Biology, College of Basic Science, Yadegar-e-Imam Khomeini(RAH) Shahre Rey Branch, Islamic Azad University, Tehran, Iran)

Received: 2016-06-06 Accepted : 2018-10-29 Published : 2016-06-06

Keywords: breast cancer, TiO2, anticancer, hydroxyapatite, Nanotube, MCF-7,

Abstract :

In this research, TiO2 nanotubes were synthesized by anodized oxidation method and were covered with a hydroxyapatite-silver nanoparticles using photodeposition and dip coating for loading silver nanoparticles and coated hydroxyapatite (HA). The morphological texture of TiO2 nanotube and Ag-HA nanoparticles on TiO2 nanotubes surface were studied by field emission scanning electron microscopy (FESEM), energy dispersive X-ray spectroscopy (EDAX analysis) and X-ray diffraction (XRD). The MCF-7 cell lines were treated with concentrations 1, 10 and 100 µg/ml of TiO2 nanotubes and HA/Ag/TiO2 nanotube for 24 and 48h. Finally, the cell viability and IC50% were evaluated using MTT assay. The results show that the HA/Ag/TiO2 has more positive effect on enhancing the cell death compare to TiO2 nanotubes and also exerts a time and concentration-dependent inhibition effect on viability of MCF-7 cells

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