Characterization of Chemical Composition and Antioxidant Properties of Trachyspermum ammi Seed as a Potential Medicinal Plant

Subject Areas : Journal of Chemical Health Risks

Hashem Akhlaghi ¹ (Department of Basic Sciences, Sabzevar Branch, Islamic Azad University, Sabzevar, Iran)
Bhnam Mahdavi ² (Department of Chemistry, Hakim Sabzevari University, Sabzevar, Iran)
Hasan Rezaei ³ (Traditional Medicine Research Center, Sabzevar University of Medical Sciences, Sabzevar, Iran)

Received: 2014-12-02 Accepted : 2018-10-29 Published : 2018-10-29

Keywords: Antioxidant Activity, GC/MS, Trachyspermum ammi, Hexadecanoic acid, Ethyl linoleate,

Abstract :

In this study, the essential oil obtained by hydrodistillation of the seed of Trachyspermum ammi (Umbelliferae), growing wild in Sabzevar, Khorasan Razavi province (Iran), were analyzed by GC and GC/MS. The yield of total volatiles was 2.3% (w/w). Forty four compounds representing 91.6% of the aerial parts oil were identified.  The main components of the oil were Hexadecanoic acid (27.5%), ethyl linoleate (8.5%), 6-methyl-α-ionone (8.0%), isobutyl phthalate (5.8%), α-cadinol (4.7%), germacrene D (4.3%) and δ-cadinene (3.5%). The oil was rich in nonterpenoids (56.0%). The total flavonoid content of different extracts of the plant was found to be in the range 53.2-164.5 mg/g while the maximum amount concern to methanol extract. The antioxidant activity of the extracts was also measured by radical scavenging activity of antioxidants against free radical 1, 1-diphenyl-2-picrylhydrazyl (DPPH) method. Results showed that IC50 of the extracts of Trachyspermum ammi seed are higher than the standard synthetic antioxidants, BHT, ascorbic acid and gallic acid.

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