تأثیر تمرینات تناوبی با شدت بالا همراه با مکمل‌سازی نانوکورکومین بر باروری و شاخص‌های اکسیداتیو در موش‌های دیابتی
محورهای موضوعی : فیزیولوژی
1 - گروه تربیت بدنی، دانشكده علوم، واحد ارومیه، دانشگاه آزاد اسلامی، ارومیه، ایران
کلید واژه: دیابت, باروری, تمرینات تناوبی با شدت بالا, استرس اکسیداتیو, نانوکورکومین,
چکیده مقاله :
این مطالعه به بررسی تأثیرات ترکیبی HIIT و مکمل‌سازی نانوکورکومین (NCC) بر کیفیت اسپرم و شاخص‌های استرس اکسیداتیو در موش‌های دیابتی می‌پردازد.چهل موش نر بالغ ویستار به پنج گروه تقسیم شدند: کنترل، دیابتی، دیابتی با نانوکورکومین، دیابتی همراه با HIIT، و دیابتی با هر دو مداخله NCC و HIIT. دیابت با استفاده از استرپتوزوتوسین (STZ) القا شد. گروه‌های NCC و HIIT به مدت 30 روز درمان‌های خود را دریافت کردند. تعداد اسپرم، تحرک، زنده‌مانی، یکپارچگی DNA، سطح تستوسترون، و نشانگرهای استرس اکسیداتیو شامل مالون‌دی‌آلدئید (MDA)، ظرفیت کلی آنتی‌اکسیدانی (TAC)، و کاتالاز (CAT) ارزیابی شد. موش‌های دیابتی کاهش قابل توجهی در تعداد اسپرم، تحرک و زنده‌مانی نشان دادند، همراه با افزایش آسیب به DNA اسپرم، کاهش سطح تستوسترون و افزایش سطح MDA. هر دو مداخله NCC و HIIT این پارامترها را بهبود بخشیدند، اما ترکیب HIIT و NCC منجر به بهبود قابل توجه‌تری شد. این بهبود شامل افزایش تعداد اسپرم، تحرک و زنده‌مانی، کاهش آسیب به DNA، افزایش سطح تستوسترون و افزایش ظرفیت آنتی‌اکسیدانی (TAC) با کاهش سطح MDA بود. استفاده ترکیبی از HIIT و مکمل‌سازی NCC به طور مؤثری کیفیت اسپرم را بهبود می‌بخشد و استرس اکسیداتیو را در موش‌های دیابتی کاهش می‌دهد. این رویکرد یک استراتژی بالقوه برای بهبود باروری مردان در شرایط دیابتی ارائه می‌دهد و نیاز به تحقیقات بیشتری برای بررسی اثربخشی و ایمنی طولانی‌مدت آن دارد.
چکیده انگلیسی:
Diabetes mellitus (DM) is associated with oxidative stress and reduced reproductive capacity in males. This study investigates the combined effects of HIIT and curcumin supplementation on sperm quality and oxidative stress parameters in diabetic rats. Forty adults male Wistar rats were divided into five groups: control, DM, DM with Nanocurcumin (NCC), DM with HIIT, and DM with both NCC and HIIT. Diabetes was induced using streptozotocin (STZ). The NCC and HIIT groups received their respective treatments for 30 days. Sperm count, motility, viability, DNA integrity, testosterone levels, and oxidative stress markers (MDA, TAC, CAT) were assessed. Diabetic rats exhibited reduced sperm count, motility, and viability, alongside increased sperm DNA damage, decreased testosterone levels, and elevated MDA levels. Both NCC and HIIT improved these parameters. The combination of NCC and HIIT resulted in the most significant improvements, including higher sperm count, motility, and viability, reduced DNA damage, increased testosterone levels, and enhanced antioxidant capacity (TAC) with decreased MDA levels. The combined use of HIIT and NCC supplementation effectively enhances sperm quality and reduces oxidative stress in diabetic rats. This integrated approach offers a potential strategy for improving male fertility in diabetic conditions, warranting further research into its long-term effectiveness and safety.
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[2] Kandemir, F.M., Ozkaraca, M., Küçükler, S., Caglayan, C., & Hanedan, B. (2018). "Preventive effects of hesperidin on diabetic nephropathy induced by streptozotocin via modulating TGF-β1 and oxidative DNA damage." Toxin Reviews, 37(4), pp. 287-293.
[3] Aksu, E.H., Kandemir, F.M., & Küçükler, S. (2021). "Ameliorative effect of hesperidin on streptozotocin-diabetes mellitus-induced testicular DNA damage and sperm quality degradation in Sprague-Dawley rats." Journal of Food Biochemistry, 45(10), p. e13938
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[5] Ghanbari, E., Nejati, V., & Azadbakht, M. (2015). "Protective effect of royal jelly against renal damage in streptozotocin-induced diabetic rats."
[6] Zhang, S., Xu, H., Yu, X., Wu, Y.I., & Sui, D. (2017). "Metformin ameliorates diabetic nephropathy in a rat model of low-dose streptozotocin-induced diabetes." Experimental and Therapeutic Medicine, 14(1), pp. 383-390.
[7] Tan, M.H., Alquraini, H., Mizokami-Stout, K., & MacEachern, M. (2016). "Metformin: from research to clinical practice." Endocrinology and Metabolism Clinics, 45(4), pp. 819-843.
[8] Saisho, Y. (2015). "Metformin and inflammation: its potential beyond glucose-lowering effect." Endocrine, Metabolic & Immune Disorders-Drug Targets, 15(3), pp. 196-205.
[9] Park, M.J., Lee, S.Y., Moon, S.J., Son, H.J., Lee, S.H., Kim, E.K., Byun, J.K., Shin, D.Y., Park, S.H., Yang, C.W., & Cho, M.L. (2016). "Metformin attenuates graft-versus-host disease via restricting mammalian target of rapamycin/signal transducer and activator of transcription 3 and promoting adenosine monophosphate–activated protein kinase-autophagy for the balance between T helper 17 and Tregs." Translational Research, 173, pp. 115-130.
[10] Fatima, M., Sadeeqa, S., & Nazir, S.U.R. (2018). "Metformin and its gastrointestinal problems: A review." Biomed Res, 29(11), pp. 2285-2289.
[11] Pourheydar, B., Azarm, F., Farjah, G., Karimipour, M., & Pourheydar, M. (2021). "Effect of silymarin and metformin on the sperm parameters and histopathological changes of testes in diabetic rats: An experimental study." International Journal of Reproductive BioMedicine, 19(12), p. 1091.
[12] Carro, E., Trejo, J.L., Busiguina, S., & Torres-Aleman, I. (2001). "Circulating insulin-like growth factor I mediates the protective effects of physical exercise against brain insults of different etiology and anatomy." Journal of Neuroscience, 21(15), pp. 5678-5684.
[13] Tillerson, J.L., Caudle, W.M., Reveron, M.E., & Miller, G.W. (2003). "Exercise induces behavioral recovery and attenuates neurochemical deficits in rodent models of Parkinson's disease." Neuroscience, 119(3), pp. 899-911.
[14] Kempf, K., Rose, B., Herder, C., Kleophas, U., Martin, S., & Kolb, H. (2006). "Inflammation in metabolic syndrome and type 2 diabetes: impact of dietary glucose." Annals of the New York Academy of Sciences, 1084(1), pp. 30-48.
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[16] Klein, C., Jonas, W., Iggena, D., Empl, L., Rivalan, M., Wiedmer, P., Spranger, J., Hellweg, R., Winter, Y., & Steiner, B. (2016). "Exercise prevents high-fat diet-induced impairment of flexible memory expression in the water maze and modulates adult hippocampal neurogenesis in mice." Neurobiology of Learning and Memory, 131, pp. 26-35.
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