Comparison of anesthesia induced by Isoflurane, Ketamine, and Ketamine-Diazepam in male rats
Shahram Saghaei
1
(
Department of Pathobiology, Faculty of Veterinary Medicine, Ur. C., Islamic Azad University, Urmia, Iran
)
Keywords: Isoflurane, Rat, Propofol, Injectable anesthetic, Diazepam,
Abstract :
Isoflurane is an inhalational anesthetic. Moreover, the combination of ketamine and diazepam is commonly used as an anesthetic agent. The present study aimed to investigate the anesthesia induced by isoflurane, ketamine, and ketamine-diazepam on anesthesia-related factors in rats. Thirty male rats were divided into three groups of ten each. Each group was anesthetized using one of the following anesthetics: isoflurane gas, ketamine, and the combination of ketamine and diazepam. The variables of heart rate, respiratory rate, body temperature, pain reflexes (ear, tail, and pedal reflexes), duration of anesthesia induction, surgical anesthesia, and movement time were recorded. According to our results, the duration of surgical anesthesia was significantly lower in the ketamine-diazepam group compared to the other groups. Moreover, the duration of anesthesia induction was shorter in the ketamine-diazepam group compared to the other groups. However, heart rate, respiratory rate, and body temperature were significantly lower in the isoflurane-only group compared to the other groups. Also, pain reflex scores were significantly higher in the isoflurane-only group compared to the ketamine-only group. However, the ketamine-only and ketamine-diazepam groups were similar in pain reflex scores. It is recommended to perform further studies comparing injectable anesthesia agents, such as propofol, with inhalational anesthesia agents.
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Allot, PP., Steward, A.: Pharmacokinetics of halothane in the dog. British Journal of Anesthesia 1976, 48:279-295.
Bailey, J., Muir, W.: pulsus alternans during halothane anesthesia in a dog veterinary surgery 1993:22 (1): 79-84.
Beliaevskii, A.F.: Intraosseous anesthesia in combination with neuroleptanalgesia in surgery of the extremities. Vestn Khir Im I Grek.1980 Nov; 125(11):123-6.
Billiar, R.P.: combine ING anesthesia in dog. Modern veterinary practice 1076: 57 (4), pp: 319 320.
Both, N.H., Mcdonald, L.E.: Veterinary Pharmacology and Therapeutics. Fifth edition. lowa, The lowa state university press 2018.
Brickman, K R., Rega, p.: A comparative study of intraosseous versus peripheral intravenous infusion of diazepam and phenobarbital in dogs. Ann Emerg Med. 1987 Oct; 16(10):1141-4.
Brock, N., Hildebrand S.V.: A comparison of three xylene-diazepam-ketamine and xylazine -guaifenesin- ketamine in cquine anesthesia. Veterinary surgery 1990: 19(6):468-474.
Cheeran J.V., et al.: Droperido -fentanyl mixture (innovar-vet) as an anesthesia in dogs. Indian Veterinaryjournal 1991; 68 (5): 483-484.
Chelley, J., Rogers, K.: Cardiovascular effects of and interaction between calcium blocking drugs and anesthetics in chronically instrumented dogs. I. Verapamil and Thiopental. Anesthesiology 1986; 64: 560-567.
Christensen, L.Q., Bonde, J.: Drug interactions with inhalational anaesthetics. Acta Anaesthesiol Scand. 1993 Apr; 37(3):231-44.
Christensen D.W., Vernon DD.: Skin necrosis complicating intraosseous infusion. Pediatr Emerg Care. 1991 Oct; 7(5):289-90.
Clark D.M., et al: cardiopulmonary responses to xylazine ketamine anesthesia in the dog. Journal of the American Animal Hospital Association 1982: 18(9):815-821.
Cucchiara, R F., Theye, R A., Michenfelder, J D.: The effects of isoflurane on canine cerebral metabolism and blood flow. Anesthesiology. 1974 Jun;40(6):571-4.
Cullen, L K., Reynoldson, J A.: Xylazine or medetomidine premedication before propofol anaesthesia. Vet Rec. 1993 Apr 10;132(15):378-83.
Dedrick, DK.: The effects of intraosseous infusion on the growth plate in a nestling rabbit model. Medicine Volume 21, Issue 5, May 1992, Pages 494-497.
DeYoung, D W et al.: Dissociative anesthetics in the cat and dog. J Am Vet Med Assoc 1972 Dec 1;161(11):1442-5.
Drrigers, D.A., Jobnson R.: emergency resuscitation in children. Emergency resuscitation 1991:89 (4): 129-132.
Evans, R.G.: Intraosseous infusion. British journal of hospital medicine 1994:51 (4): 161-164.
Ewing, K., Mohammed, H O.: Reduction of isoflurane anesthetic requirement by medetomidine and its restoration by atipamezole in dogs. Am J Vet Res. 1993 Feb;54(2):294-9.
Faggella, A M., Aronsohn, M G.: Anesthetic techniques for neutering 6- to 14-week-old kittens. J Am Vet Med Assoc. 1993 Jan 1;202(1):56-62.
Goel, S., Adetunji, A.: Effect of single and incremental doses of thiopentone anesthesia in dog. Indian veterinary journal 1991: 68: 451-454.
Goldstein, B., Doody, D.: Emergency intraosseous infusion in severely burned children. Pediatr Emerg Care. 1990 Sep;6(3):195-7.
Golenz, M R., Carlson, G P.: Preliminary report: the development of an intraosseous infusion technique for neonatal foals. J Vet Intern Med. 1993 Nov-Dec;7(6):377-82.
Green, C.J.: Laboratory animal hand book. animal anesthesia. london: laboratory animal LTD 2010: 10-14, 29-43, 53-63.
Greene, S.A. Veterinary Anesthesia and Pain Manangement Secrets.Hanley & Belfus Inc., Philadelphia, 2012. PP: 87-90, 91-95.
Grodin, W K., Epstein, M A.: Soda lime adsorption of isoflurane and enflurane. Anesthesiology 1985 Jan;62(1):60-4.
Gross, M E., Tranquilli, W J., et al.: Yohimbine/flumazenil antagonism of hemodynamic alterations induced by a combination of midazolam, xylazine, and butorphanol in dogs. J Am Vet Med Assoc. 1992 Dec 15;201(12):1887-90.
Gross, M E., Jones, B D.: Effects of abdominal insufflation with nitrous oxide on cardiorespiratory measurements in spontaneously breathing isoflurane-anesthetized dogs. Am J Vet Res . 1993 Aug;54(8):1352-8.
Gross, M E., Smith, J A.: Cardiorespiratory effects of combined midazolam and butorphanol in isoflurane-anesthetized cats. Vet Surg. 1993 Mar-Apr;22(2):159-62.
Hall, L W.: Effects of nitrous oxide on respiration during halothane anaesthesia in the dog. Br J Anaesth. 1988 Feb;60(2):207-15.
Hall, L.W.: wright's veterinary anesthesia and analgesia, seventh edition, Baillier Tindall & cassel, 2016:146, 161-165, 195-200, 213-230, 248-278, 318.319,362-383.
Hall, L.W., Clark K.W.: Veterinary anesthesia. ninth edition, Baillier Tindall, 2014:38-45, 50-90, 98-110,305-315,334-336, 347.
Hatch, R C.: Effect of autonomic blocking agents on development of acute tolerance to thiopental in dogs. Am J Vet Res. 1972 Feb;33(2):365-76.
Hatch, R C. et al.: Comparison of five preanesthetic medicaments in thiopental-anesthetized cats: antagonism by selected compounds. Am J Vet Res. 1984 Nov;45(11):2322-7.
Heard, D J., Webb, A I.: Effect of acepromazine on the anesthetic requirement of halothane in the dog. Am J Vet Res. 1986 Oct;47(10):2113-5.
Hellebrekers, L J.: Comparison of isoflurane and halothane as inhalation anaesthetics in the dog. Vet Q. 1986 Jul;8(3):183-8.
Hellyer, P W., et al.: Induction of anesthesia with diazepam-ketamine and midazolam-ketamine in greyhounds. Vet Surg. 1991 Mar-Apr;20(2):143-7.