کاربرد روش ميکرواستخراج فاز جامد با فيبر پوششی کورکومين/پلی پيرول برای اندازه گيری سموم کشاورزی به وسیله سوانگاری گازی
محورهای موضوعی : شیمی تجزیهعاطفه نورا 1 , حمید احمر 2 , مصطفی جعفری 3 , سید محمدحسین بنی‌طبا 4
1 - دانشجوی کارشناسی ارشد گروه شيمي، دانشكده علوم پايه، دانشگاه زابل، زابل، ايران.
2 - دانشیار گروه شيمي، دانشكده علوم پايه، دانشگاه زابل، زابل، ايران.
3 - استادیار دانشكده علوم شيمي و نفت، دانشگاه شهيدبهشتي، تهران، ايران.
4 - استادیار موسسه تحقيقات واکسن و سرم‌سازی رازی، سازمان تحقيقات، آموزش و ترويج کشاورزی، کرج، ايران.
کلید واژه: ميكرواستخراج فاز جامد, كوركومين, سموم كشاورزي, بوتاکلر, سوانگاری گازي.,
چکیده مقاله :
در اين پژوهش، امکان به کارگیری روش ميکرواستخراج فاز جامد به¬روش مستقيم با يک فيبر جديد برای پيش تغليظ سم بوتاکلر و اندازه-گيری آن به¬روش سوانگاری گازی بررسی شده است. برای استخراج اين آفت کش از نمونه های آبی از کورکومين و پلی پيرول به عنوان جاذب برای تهيه فيبر ميکرواستخراج فاز جامد استفاده شد و برای اين منظور بسپارش الکتروشيميايی به روش ولت آمپرسنجی چرخه ای به ارگرفته شد. ويژگی های فيبر جديد مانند چسبندگی، استحکام مکانيکی و پايداری گرمایی ارزیابی و ریخت¬شناسی آن با میکروسکوپ الکترونی روبشی (SEM)، بررسی شد. در ادامه، عامل¬های موثر بر مقدار استخراج بوتاکلر از نمونه های آبی شامل زمان استخراج، دمای استخراج، pH و اثر افزودن نمک ارزيابی شد. درشرايط بهينه، اعداد شايستگی روش پيشنهادی شامل حد تشخيص، گستره خطی، تکرارپذيری در يک روز و بين چند روز ارزيابی شد. حد تشخيص روش برابر با 1 نانوگرم بر ليتر، گستره خطی 067/0 تا 40 ميکروگرم بر ليتر، تکرارپذيری در يک روز 9/2 درصد و تکرارپذيری بين چند روز برابر با 0/5 درصد به¬دست آمد. روش توسعه داده شده برای تجزیه نمونه¬های آب شاليزارهای منطقه شمال ايران (گنبد کاووس) به عنوان نمونه حقيقی به کارگرفته شد.
In this study, the possibility of using solid phase microextraction method directly with a new fiber for preconcentration and measurement of butachlor pesticide by gas chromatography was investigated. Polypyrrole/ curcumin coated fiber was used as an adsorbent to prepare the solid phase microextraction fiber from aqueous samples and electrochemical polymerization was employed using cyclic voltammetry method. The properties of the new fiber including adhesion, mechanical strength, and thermal stability were evaluated. Morphology of the prepared sample was studied using scanning electron microscope (SEM). Then the effective parameters on the extraction efficiency of butachlor from aqueous samples including extraction time, extraction temperature, and pH were evaluated. Under optimized conditions, the performance characteristics of the proposed method including detection limit, linear range, and intera day and inter days repeatability were evaluated. The detection limit of the method was found to be 1 ng/l, linear range was 0.067-40 g/l, repeatability intra day was 2.9% and repeatability inter days was 5.0 %. The developed method was used for analysis of water samples from rice fields in northern Iran region as a real sample.
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